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Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus

Identification of a gain-of-function mutant under PEG-induced water stress and soil-water stress. (A) Twenty-one-day-old seedlings of the selected mutant screened in pots by withholding irrigation for 6 days. (B) The extent of recovery of the mutant after 4 days of the recovery period.
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PLV023F1: Identification of a gain-of-function mutant under PEG-induced water stress and soil-water stress. (A) Twenty-one-day-old seedlings of the selected mutant screened in pots by withholding irrigation for 6 days. (B) The extent of recovery of the mutant after 4 days of the recovery period.

Mentions: We identified a mutant, ewst1 that showed enhanced tolerance with a 90–100 % survival rate when compared with its WT Nagina22 under PEG (6000) stress [see Supporting Information—Fig. S2A and B]. This mutant exhibited enhanced tolerance in pot screening carried out under soil-water stress than the WT in terms of percentage of leaf rolling, leaf drying and recovery rate. In the drought scoring scale, ewst1 had a low score (score 0), indicating better tolerance, whereas the WT was susceptible with a higher score (score 7) under stress (Fig. 1A). It also recovered better with a recovery rate of 92.6 % when compared with Nagina22 (15.3 %) (Fig. 1B).Figure 1.


Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance.

Lima JM, Nath M, Dokku P, Raman KV, Kulkarni KP, Vishwakarma C, Sahoo SP, Mohapatra UB, Mithra SV, Chinnusamy V, Robin S, Sarla N, Seshashayee M, Singh K, Singh AK, Singh NK, Sharma RP, Mohapatra T - AoB Plants (2015)

Identification of a gain-of-function mutant under PEG-induced water stress and soil-water stress. (A) Twenty-one-day-old seedlings of the selected mutant screened in pots by withholding irrigation for 6 days. (B) The extent of recovery of the mutant after 4 days of the recovery period.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482838&req=5

PLV023F1: Identification of a gain-of-function mutant under PEG-induced water stress and soil-water stress. (A) Twenty-one-day-old seedlings of the selected mutant screened in pots by withholding irrigation for 6 days. (B) The extent of recovery of the mutant after 4 days of the recovery period.
Mentions: We identified a mutant, ewst1 that showed enhanced tolerance with a 90–100 % survival rate when compared with its WT Nagina22 under PEG (6000) stress [see Supporting Information—Fig. S2A and B]. This mutant exhibited enhanced tolerance in pot screening carried out under soil-water stress than the WT in terms of percentage of leaf rolling, leaf drying and recovery rate. In the drought scoring scale, ewst1 had a low score (score 0), indicating better tolerance, whereas the WT was susceptible with a higher score (score 7) under stress (Fig. 1A). It also recovered better with a recovery rate of 92.6 % when compared with Nagina22 (15.3 %) (Fig. 1B).Figure 1.

Bottom Line: It exhibited increase in maximum root length without any significant changes in its root weight, root volume and total root number on crown when compared with the WT under stress in PVC tube experiment.Root anatomy and stomatal microscopic studies revealed changes in the number of xylem and phloem cells, size of central meta-xylem and number of closed stomata in ewst1.The possible involvement of a candidate gene with respect to the observed morpho-physiological and transcriptional changes and its role in stress tolerance are discussed.

View Article: PubMed Central - PubMed

Affiliation: National Research Centre on Plant Biotechnology, IARI, New Delhi, India Department of Botany, North Orissa University, Baripada, Odisha, India.

No MeSH data available.


Related in: MedlinePlus