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Leucine supplementation improves leptin sensitivity in high-fat diet fed rats.

Yuan XW, Han SF, Zhang JW, Xu JY, Qin LQ - Food Nutr Res (2015)

Bottom Line: No significant differences in body weight and food/energy intake existed among the four groups.These phenomena suggested that leptin sensitivity was improved in the leucine groups.Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China.

ABSTRACT

Background: Several studies have reported the favorable effect of leucine supplementation on insulin resistance or insulin sensitivity. However, whether or not leucine supplementation improves leptin sensitivity remains unclear.

Design: Forty-eight male Sprague-Dawley rats were fed with either a high-fat diet (HFD) or HFD supplemented with 1.5, 3.0, and 4.5% leucine for 16 weeks. At the end of the experiment, serum leptin level was measured by ELISA, and leptin receptor (ObR) in the hypothalamus was examined by immunohistochemistry. The protein expressions of ObR and leptin-signaling pathway in adipose tissues were detected by western blot.

Results: No significant differences in body weight and food/energy intake existed among the four groups. Serum leptin levels were significantly lower, and ObR expression in the hypothalamus and adipose tissues was significantly higher in the three leucine groups than in the control group. These phenomena suggested that leptin sensitivity was improved in the leucine groups. Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

Conclusions: Leucine supplementation improves leptin sensitivity in rats on HFD likely by promoting leptin signaling.

No MeSH data available.


Related in: MedlinePlus

The representative protein expression of ObR, JAK2, STAT3, and SOCS3 in the four groups. Values are means for 12 rats with SD represented by vertical bars. The intensity of the bands was normalized using each corresponding β-actin density. The mean value was significantly different from that of the HFD group at ##P<0.01, from that of the HFD+1.5% Leu group at **P<0.01, and from that of the HFD+3.0% Leu group at &&P<0.01. HFD, high-food diet; Leu, leucine.
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Figure 0004: The representative protein expression of ObR, JAK2, STAT3, and SOCS3 in the four groups. Values are means for 12 rats with SD represented by vertical bars. The intensity of the bands was normalized using each corresponding β-actin density. The mean value was significantly different from that of the HFD group at ##P<0.01, from that of the HFD+1.5% Leu group at **P<0.01, and from that of the HFD+3.0% Leu group at &&P<0.01. HFD, high-food diet; Leu, leucine.

Mentions: The effects of supplemental leucine on the leptin-signaling pathway in adipose tissues were shown in Fig. 4. The protein expression of ObR, JAK2, and STAT3 was significantly higher in the three leucine groups than in the HFD group (all P<0.01). However, SOCS3 expression was significantly lower in the HFD+1.5% Leu and HFD+4.5% Leu groups than in the HFD group (P<0.01). Notably, the expression of ObR, JAK2, and STAT3 was the highest, and the expression of SOCS3 was the lowest in HFD+1.5% Leu group (P<0.01, compared with the HFD+3.0% Leu and HFD+4.5% Leu groups).


Leucine supplementation improves leptin sensitivity in high-fat diet fed rats.

Yuan XW, Han SF, Zhang JW, Xu JY, Qin LQ - Food Nutr Res (2015)

The representative protein expression of ObR, JAK2, STAT3, and SOCS3 in the four groups. Values are means for 12 rats with SD represented by vertical bars. The intensity of the bands was normalized using each corresponding β-actin density. The mean value was significantly different from that of the HFD group at ##P<0.01, from that of the HFD+1.5% Leu group at **P<0.01, and from that of the HFD+3.0% Leu group at &&P<0.01. HFD, high-food diet; Leu, leucine.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482813&req=5

Figure 0004: The representative protein expression of ObR, JAK2, STAT3, and SOCS3 in the four groups. Values are means for 12 rats with SD represented by vertical bars. The intensity of the bands was normalized using each corresponding β-actin density. The mean value was significantly different from that of the HFD group at ##P<0.01, from that of the HFD+1.5% Leu group at **P<0.01, and from that of the HFD+3.0% Leu group at &&P<0.01. HFD, high-food diet; Leu, leucine.
Mentions: The effects of supplemental leucine on the leptin-signaling pathway in adipose tissues were shown in Fig. 4. The protein expression of ObR, JAK2, and STAT3 was significantly higher in the three leucine groups than in the HFD group (all P<0.01). However, SOCS3 expression was significantly lower in the HFD+1.5% Leu and HFD+4.5% Leu groups than in the HFD group (P<0.01). Notably, the expression of ObR, JAK2, and STAT3 was the highest, and the expression of SOCS3 was the lowest in HFD+1.5% Leu group (P<0.01, compared with the HFD+3.0% Leu and HFD+4.5% Leu groups).

Bottom Line: No significant differences in body weight and food/energy intake existed among the four groups.These phenomena suggested that leptin sensitivity was improved in the leucine groups.Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China.

ABSTRACT

Background: Several studies have reported the favorable effect of leucine supplementation on insulin resistance or insulin sensitivity. However, whether or not leucine supplementation improves leptin sensitivity remains unclear.

Design: Forty-eight male Sprague-Dawley rats were fed with either a high-fat diet (HFD) or HFD supplemented with 1.5, 3.0, and 4.5% leucine for 16 weeks. At the end of the experiment, serum leptin level was measured by ELISA, and leptin receptor (ObR) in the hypothalamus was examined by immunohistochemistry. The protein expressions of ObR and leptin-signaling pathway in adipose tissues were detected by western blot.

Results: No significant differences in body weight and food/energy intake existed among the four groups. Serum leptin levels were significantly lower, and ObR expression in the hypothalamus and adipose tissues was significantly higher in the three leucine groups than in the control group. These phenomena suggested that leptin sensitivity was improved in the leucine groups. Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

Conclusions: Leucine supplementation improves leptin sensitivity in rats on HFD likely by promoting leptin signaling.

No MeSH data available.


Related in: MedlinePlus