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Leucine supplementation improves leptin sensitivity in high-fat diet fed rats.

Yuan XW, Han SF, Zhang JW, Xu JY, Qin LQ - Food Nutr Res (2015)

Bottom Line: No significant differences in body weight and food/energy intake existed among the four groups.These phenomena suggested that leptin sensitivity was improved in the leucine groups.Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China.

ABSTRACT

Background: Several studies have reported the favorable effect of leucine supplementation on insulin resistance or insulin sensitivity. However, whether or not leucine supplementation improves leptin sensitivity remains unclear.

Design: Forty-eight male Sprague-Dawley rats were fed with either a high-fat diet (HFD) or HFD supplemented with 1.5, 3.0, and 4.5% leucine for 16 weeks. At the end of the experiment, serum leptin level was measured by ELISA, and leptin receptor (ObR) in the hypothalamus was examined by immunohistochemistry. The protein expressions of ObR and leptin-signaling pathway in adipose tissues were detected by western blot.

Results: No significant differences in body weight and food/energy intake existed among the four groups. Serum leptin levels were significantly lower, and ObR expression in the hypothalamus and adipose tissues was significantly higher in the three leucine groups than in the control group. These phenomena suggested that leptin sensitivity was improved in the leucine groups. Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

Conclusions: Leucine supplementation improves leptin sensitivity in rats on HFD likely by promoting leptin signaling.

No MeSH data available.


Related in: MedlinePlus

immunohistochemical staining of hypothalamic ObR (a) and comparison (b) in the four groups. (a) Original magnification, 400×. (b) Values are means for four rats with SD represented by vertical bars. The mean value was significantly different from that of the HFD group at ##P<0.01 and from that of the HFD+1.5% Leu group at *P<0.05. HFD, high-food diet; Leu, leucine.
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Figure 0003: immunohistochemical staining of hypothalamic ObR (a) and comparison (b) in the four groups. (a) Original magnification, 400×. (b) Values are means for four rats with SD represented by vertical bars. The mean value was significantly different from that of the HFD group at ##P<0.01 and from that of the HFD+1.5% Leu group at *P<0.05. HFD, high-food diet; Leu, leucine.

Mentions: Hypothalamic ObR was highly expressed in the rats supplemented with leucine (Fig. 3a). The count of ObR expression was 60.73±8.22, 99.35±9.15, 82.48±9.12, and 80.31±7.49 in the HFD, HFD+1.5% Leu, HFD+3.0% Leu, and HFD+4.5% Leu groups, respectively. The expression was significantly higher in the three leucine groups than in the HFD group (all P<0.01). Furthermore, the expression was significantly higher in the HFD+1.5% Leu group than in the HFD+3.0% Leu or HFD+4.5% Leu group (both P<0.05) (Fig. 3b).


Leucine supplementation improves leptin sensitivity in high-fat diet fed rats.

Yuan XW, Han SF, Zhang JW, Xu JY, Qin LQ - Food Nutr Res (2015)

immunohistochemical staining of hypothalamic ObR (a) and comparison (b) in the four groups. (a) Original magnification, 400×. (b) Values are means for four rats with SD represented by vertical bars. The mean value was significantly different from that of the HFD group at ##P<0.01 and from that of the HFD+1.5% Leu group at *P<0.05. HFD, high-food diet; Leu, leucine.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482813&req=5

Figure 0003: immunohistochemical staining of hypothalamic ObR (a) and comparison (b) in the four groups. (a) Original magnification, 400×. (b) Values are means for four rats with SD represented by vertical bars. The mean value was significantly different from that of the HFD group at ##P<0.01 and from that of the HFD+1.5% Leu group at *P<0.05. HFD, high-food diet; Leu, leucine.
Mentions: Hypothalamic ObR was highly expressed in the rats supplemented with leucine (Fig. 3a). The count of ObR expression was 60.73±8.22, 99.35±9.15, 82.48±9.12, and 80.31±7.49 in the HFD, HFD+1.5% Leu, HFD+3.0% Leu, and HFD+4.5% Leu groups, respectively. The expression was significantly higher in the three leucine groups than in the HFD group (all P<0.01). Furthermore, the expression was significantly higher in the HFD+1.5% Leu group than in the HFD+3.0% Leu or HFD+4.5% Leu group (both P<0.05) (Fig. 3b).

Bottom Line: No significant differences in body weight and food/energy intake existed among the four groups.These phenomena suggested that leptin sensitivity was improved in the leucine groups.Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China.

ABSTRACT

Background: Several studies have reported the favorable effect of leucine supplementation on insulin resistance or insulin sensitivity. However, whether or not leucine supplementation improves leptin sensitivity remains unclear.

Design: Forty-eight male Sprague-Dawley rats were fed with either a high-fat diet (HFD) or HFD supplemented with 1.5, 3.0, and 4.5% leucine for 16 weeks. At the end of the experiment, serum leptin level was measured by ELISA, and leptin receptor (ObR) in the hypothalamus was examined by immunohistochemistry. The protein expressions of ObR and leptin-signaling pathway in adipose tissues were detected by western blot.

Results: No significant differences in body weight and food/energy intake existed among the four groups. Serum leptin levels were significantly lower, and ObR expression in the hypothalamus and adipose tissues was significantly higher in the three leucine groups than in the control group. These phenomena suggested that leptin sensitivity was improved in the leucine groups. Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

Conclusions: Leucine supplementation improves leptin sensitivity in rats on HFD likely by promoting leptin signaling.

No MeSH data available.


Related in: MedlinePlus