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Leucine supplementation improves leptin sensitivity in high-fat diet fed rats.

Yuan XW, Han SF, Zhang JW, Xu JY, Qin LQ - Food Nutr Res (2015)

Bottom Line: No significant differences in body weight and food/energy intake existed among the four groups.These phenomena suggested that leptin sensitivity was improved in the leucine groups.Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China.

ABSTRACT

Background: Several studies have reported the favorable effect of leucine supplementation on insulin resistance or insulin sensitivity. However, whether or not leucine supplementation improves leptin sensitivity remains unclear.

Design: Forty-eight male Sprague-Dawley rats were fed with either a high-fat diet (HFD) or HFD supplemented with 1.5, 3.0, and 4.5% leucine for 16 weeks. At the end of the experiment, serum leptin level was measured by ELISA, and leptin receptor (ObR) in the hypothalamus was examined by immunohistochemistry. The protein expressions of ObR and leptin-signaling pathway in adipose tissues were detected by western blot.

Results: No significant differences in body weight and food/energy intake existed among the four groups. Serum leptin levels were significantly lower, and ObR expression in the hypothalamus and adipose tissues was significantly higher in the three leucine groups than in the control group. These phenomena suggested that leptin sensitivity was improved in the leucine groups. Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

Conclusions: Leucine supplementation improves leptin sensitivity in rats on HFD likely by promoting leptin signaling.

No MeSH data available.


Related in: MedlinePlus

Body weights of rats in the four groups during the experimental period (n=12 for each group). HFD, high-food diet; Leu, leucine.
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Figure 0001: Body weights of rats in the four groups during the experimental period (n=12 for each group). HFD, high-food diet; Leu, leucine.

Mentions: The body weight in the four groups was similar at the baseline and constantly increased over time (Fig. 1). At the end of the experiment, the body weight was slightly low in the HFD+1.5% Leu and HFD+3.0% Leu groups, but no significant difference with the HFD group was observed. Adipose tissue weight was not significantly different among the groups with large variation in group. Despite the slightly low intake in the three leucine groups, no significant differences in food and energy intake existed among the four groups (Table 1).


Leucine supplementation improves leptin sensitivity in high-fat diet fed rats.

Yuan XW, Han SF, Zhang JW, Xu JY, Qin LQ - Food Nutr Res (2015)

Body weights of rats in the four groups during the experimental period (n=12 for each group). HFD, high-food diet; Leu, leucine.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482813&req=5

Figure 0001: Body weights of rats in the four groups during the experimental period (n=12 for each group). HFD, high-food diet; Leu, leucine.
Mentions: The body weight in the four groups was similar at the baseline and constantly increased over time (Fig. 1). At the end of the experiment, the body weight was slightly low in the HFD+1.5% Leu and HFD+3.0% Leu groups, but no significant difference with the HFD group was observed. Adipose tissue weight was not significantly different among the groups with large variation in group. Despite the slightly low intake in the three leucine groups, no significant differences in food and energy intake existed among the four groups (Table 1).

Bottom Line: No significant differences in body weight and food/energy intake existed among the four groups.These phenomena suggested that leptin sensitivity was improved in the leucine groups.Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou, China.

ABSTRACT

Background: Several studies have reported the favorable effect of leucine supplementation on insulin resistance or insulin sensitivity. However, whether or not leucine supplementation improves leptin sensitivity remains unclear.

Design: Forty-eight male Sprague-Dawley rats were fed with either a high-fat diet (HFD) or HFD supplemented with 1.5, 3.0, and 4.5% leucine for 16 weeks. At the end of the experiment, serum leptin level was measured by ELISA, and leptin receptor (ObR) in the hypothalamus was examined by immunohistochemistry. The protein expressions of ObR and leptin-signaling pathway in adipose tissues were detected by western blot.

Results: No significant differences in body weight and food/energy intake existed among the four groups. Serum leptin levels were significantly lower, and ObR expression in the hypothalamus and adipose tissues was significantly higher in the three leucine groups than in the control group. These phenomena suggested that leptin sensitivity was improved in the leucine groups. Furthermore, the expressions of JAK2 and STAT3 (activated by ObR) were significantly higher, and that of SOCS3 (inhibits leptin signaling) was significantly lower in the three leucine groups than in the control group.

Conclusions: Leucine supplementation improves leptin sensitivity in rats on HFD likely by promoting leptin signaling.

No MeSH data available.


Related in: MedlinePlus