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Dietary Lipid Levels Influence Lipid Deposition in the Liver of Large Yellow Croaker (Larimichthys crocea) by Regulating Lipoprotein Receptors, Fatty Acid Uptake and Triacylglycerol Synthesis and Catabolism at the Transcriptional Level.

Yan J, Liao K, Wang T, Mai K, Xu W, Ai Q - PLoS ONE (2015)

Bottom Line: However, no information is available on the mechanism by which dietary lipid levels comprehensively regulate lipid transport, uptake, synthesis and catabolism in fish.Growth performance was not significantly affected.In fish fed the low-lipid diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control diet.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Ocean University of China, Qingdao, Shandong province, People's Republic of China; The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong province, People's Republic of China.

ABSTRACT
Ectopic lipid accumulation has been observed in fish fed a high-lipid diet. However, no information is available on the mechanism by which dietary lipid levels comprehensively regulate lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insight into how dietary lipids affect lipid deposition in the liver of large yellow croaker(Larimichthys crocea). Fish (150.00±4.95 g) were fed a diet with a low (6%), moderate (12%, the control diet) or high (18%) crude lipid content for 10 weeks. Growth performance, plasma biochemical indexes, lipid contents and gene expression related to lipid deposition, including lipoprotein assembly and clearance, fatty acid uptake and triacylglycerol synthesis and catabolism, were assessed. Growth performance was not significantly affected. However, the hepato-somatic and viscera-somatic indexes as well as plasma triacylglycerol, non-esterified fatty acids and LDL-cholesterol levels were significantly increased in fish fed the high-lipid diet. In the livers of fish fed the high-lipid diet, the expression of genes related to lipoprotein clearance (LDLR) and fatty acid uptake (FABP11) was significantly up-regulated, whereas the expression of genes involved in lipoprotein assembly (apoB100), triacylglycerol synthesis and catabolism (DGAT2, CPT I) was significantly down-regulated compared with fish fed the control diet, and hepatic lipid deposition increased. In fish fed the low-lipid diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control diet. However, hepatic lipid content in fish fed the low-lipid diet decreased mainly due to low dietary lipid intake. In summary, findings of this study provide molecular insight into the role of lipid deposition in the liver in response to different dietary lipid contents.

No MeSH data available.


Related in: MedlinePlus

Expression of genes related totriacylglycerol synthesis and catabolism in the liver of large yellow croaker.Values (means±S.E.M.) in bars that have the same letter are not significantly different (P>0.05; T test for FAS, DGAT2 and CPT I in the liver, and Tukey’s test for the other genes) among treatments (n = 3). FAS: fatty acid synthase; DGAT2: acyl-CoA:diacylglycerol acyltransferase 2; ATGL: adipose triglyceride lipase; CPT I: carnitine palmitoyltransferase I; ACO: acyl-CoA oxidase.
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pone.0129937.g004: Expression of genes related totriacylglycerol synthesis and catabolism in the liver of large yellow croaker.Values (means±S.E.M.) in bars that have the same letter are not significantly different (P>0.05; T test for FAS, DGAT2 and CPT I in the liver, and Tukey’s test for the other genes) among treatments (n = 3). FAS: fatty acid synthase; DGAT2: acyl-CoA:diacylglycerol acyltransferase 2; ATGL: adipose triglyceride lipase; CPT I: carnitine palmitoyltransferase I; ACO: acyl-CoA oxidase.

Mentions: In fish fed the high-lipid diet, the expression of DGAT2 and CPT I was significantly down-regulated compared with fish fed the control diet (P<0.05), whereas the expression of FAS, ATGL and ACO was not significantly altered between fish fed the high-lipid and control diets (P>0.05)(Fig 4). In fish fed the low-lipid diet, FAS and ACO were significantly up-regulated, whereas ATGL and CPT I were down-regulated compared with fish fed the control diet (P<0.05). TAG synthesis related genes (FAS and DGAT2) were expressed at higher levels in the low-lipid group compared with the high-lipid group, whereas genes related to TAG catabolism (ATGL and CPT I) were expressed at higher levels in the high-lipid group with the exception of ACO (P<0.05).


Dietary Lipid Levels Influence Lipid Deposition in the Liver of Large Yellow Croaker (Larimichthys crocea) by Regulating Lipoprotein Receptors, Fatty Acid Uptake and Triacylglycerol Synthesis and Catabolism at the Transcriptional Level.

Yan J, Liao K, Wang T, Mai K, Xu W, Ai Q - PLoS ONE (2015)

Expression of genes related totriacylglycerol synthesis and catabolism in the liver of large yellow croaker.Values (means±S.E.M.) in bars that have the same letter are not significantly different (P>0.05; T test for FAS, DGAT2 and CPT I in the liver, and Tukey’s test for the other genes) among treatments (n = 3). FAS: fatty acid synthase; DGAT2: acyl-CoA:diacylglycerol acyltransferase 2; ATGL: adipose triglyceride lipase; CPT I: carnitine palmitoyltransferase I; ACO: acyl-CoA oxidase.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482732&req=5

pone.0129937.g004: Expression of genes related totriacylglycerol synthesis and catabolism in the liver of large yellow croaker.Values (means±S.E.M.) in bars that have the same letter are not significantly different (P>0.05; T test for FAS, DGAT2 and CPT I in the liver, and Tukey’s test for the other genes) among treatments (n = 3). FAS: fatty acid synthase; DGAT2: acyl-CoA:diacylglycerol acyltransferase 2; ATGL: adipose triglyceride lipase; CPT I: carnitine palmitoyltransferase I; ACO: acyl-CoA oxidase.
Mentions: In fish fed the high-lipid diet, the expression of DGAT2 and CPT I was significantly down-regulated compared with fish fed the control diet (P<0.05), whereas the expression of FAS, ATGL and ACO was not significantly altered between fish fed the high-lipid and control diets (P>0.05)(Fig 4). In fish fed the low-lipid diet, FAS and ACO were significantly up-regulated, whereas ATGL and CPT I were down-regulated compared with fish fed the control diet (P<0.05). TAG synthesis related genes (FAS and DGAT2) were expressed at higher levels in the low-lipid group compared with the high-lipid group, whereas genes related to TAG catabolism (ATGL and CPT I) were expressed at higher levels in the high-lipid group with the exception of ACO (P<0.05).

Bottom Line: However, no information is available on the mechanism by which dietary lipid levels comprehensively regulate lipid transport, uptake, synthesis and catabolism in fish.Growth performance was not significantly affected.In fish fed the low-lipid diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control diet.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), Ocean University of China, Qingdao, Shandong province, People's Republic of China; The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong province, People's Republic of China.

ABSTRACT
Ectopic lipid accumulation has been observed in fish fed a high-lipid diet. However, no information is available on the mechanism by which dietary lipid levels comprehensively regulate lipid transport, uptake, synthesis and catabolism in fish. Therefore, the present study aimed to gain further insight into how dietary lipids affect lipid deposition in the liver of large yellow croaker(Larimichthys crocea). Fish (150.00±4.95 g) were fed a diet with a low (6%), moderate (12%, the control diet) or high (18%) crude lipid content for 10 weeks. Growth performance, plasma biochemical indexes, lipid contents and gene expression related to lipid deposition, including lipoprotein assembly and clearance, fatty acid uptake and triacylglycerol synthesis and catabolism, were assessed. Growth performance was not significantly affected. However, the hepato-somatic and viscera-somatic indexes as well as plasma triacylglycerol, non-esterified fatty acids and LDL-cholesterol levels were significantly increased in fish fed the high-lipid diet. In the livers of fish fed the high-lipid diet, the expression of genes related to lipoprotein clearance (LDLR) and fatty acid uptake (FABP11) was significantly up-regulated, whereas the expression of genes involved in lipoprotein assembly (apoB100), triacylglycerol synthesis and catabolism (DGAT2, CPT I) was significantly down-regulated compared with fish fed the control diet, and hepatic lipid deposition increased. In fish fed the low-lipid diet, the expression of genes associated with lipoprotein assembly and clearance (apoB100, LDLR, LRP-1), fatty acid uptake (CD36, FATP1, FABP3) and triacylglycerol synthesis (FAS) was significantly increased, whereas the expression of triacylglycerol catabolism related genes (ATGL, CPT I) was reduced compared with fish fed the control diet. However, hepatic lipid content in fish fed the low-lipid diet decreased mainly due to low dietary lipid intake. In summary, findings of this study provide molecular insight into the role of lipid deposition in the liver in response to different dietary lipid contents.

No MeSH data available.


Related in: MedlinePlus