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Investigation of a Large Collection of Pseudomonas aeruginosa Bacteriophages Collected from a Single Environmental Source in Abidjan, Côte d'Ivoire.

Essoh C, Latino L, Midoux C, Blouin Y, Loukou G, Nguetta SP, Lathro S, Cablanmian A, Kouassi AK, Vergnaud G, Pourcel C - PLoS ONE (2015)

Bottom Line: The vast majority of the genetic variations among virulent phages from the same genus resulted from seemingly non-random horizontal transfer events, inside a population of P. aeruginosa phages with limited diversity.This is the first study of P. aeruginosa phage diversity in an African city and it shows that a large variety of phage species can be recovered in a limited geographical site at least when different bacterial strains are used.The relative temporal and spatial stability of the Abidjan phage population might reflect equilibrium in the microbial community from which they are released.

View Article: PubMed Central - PubMed

Affiliation: Institute for Integrative Biology of the Cell, CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, Orsay, France.

ABSTRACT
Twenty two distinct bacteriophages were isolated from sewage water from five locations in the city of Abidjan, Côte d'Ivoire over a two-year period, using a collection of Pseudomonas aeruginosa strains with diverse genotypes. The phages were characterized by their virulence spectrum on a panel of selected P. aeruginosa strains from cystic fibrosis patients and by whole genome sequencing. Twelve virions representing the observed diversity were visualised by electron microscopy. The combined observations showed that 17 phages, distributed into seven genera, were virulent, and that five phages were related to temperate phages belonging to three genera. Some showed similarity with known phages only at the protein level. The vast majority of the genetic variations among virulent phages from the same genus resulted from seemingly non-random horizontal transfer events, inside a population of P. aeruginosa phages with limited diversity. This suggests the existence of a single environmental reservoir or ecotype in which continuous selection is taking place. In contrast, mostly point mutations were observed among phages potentially capable of lysogenisation. This is the first study of P. aeruginosa phage diversity in an African city and it shows that a large variety of phage species can be recovered in a limited geographical site at least when different bacterial strains are used. The relative temporal and spatial stability of the Abidjan phage population might reflect equilibrium in the microbial community from which they are released.

No MeSH data available.


Related in: MedlinePlus

Electron microscopy examination of nine phages representative of the different genera observed in the present phage collection.Scale bar represents 100 nm.
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pone.0130548.g012: Electron microscopy examination of nine phages representative of the different genera observed in the present phage collection.Scale bar represents 100 nm.

Mentions: EM examination was performed for at least one phage of each genus (Fig 12). As expected from the genome sequence, phages belonging to the PAK-P1, KPP10 and PB1 genera were myoviruses (A1 morphotype) with long contractile tails. Five PAK-P1-like phages were analysed and all showed the same morphology, with a 130 nm tail and a 67–70 nm head. Ab11, a KPP10-like phage, had a 70 nm head and a 120nm long tail, while virion particles of Ab29, a PB1-like phage, possessed a 74 nm head and a 140 nm long tail. Among podoviruses with short tails (C1 morphotype), Ab05 and Ab22, belonging to the ɸKMV-like and LUZ24-like genera respectively, displayed 60nm icosahedral heads, while Ab09, a N4-like phage, had a 70 nm icosahedral head. Ab18, Ab26 and Ab30 were siphoviruses with a B1 morphotype. Ab18 showed an elongated 85×60nm head and a 130 nm tail similar to that of phage YuA. Ab26 had a head of 60 nm and tail of 170 nm, Ab30 a head of 55 nm and tail of 180 nm.


Investigation of a Large Collection of Pseudomonas aeruginosa Bacteriophages Collected from a Single Environmental Source in Abidjan, Côte d'Ivoire.

Essoh C, Latino L, Midoux C, Blouin Y, Loukou G, Nguetta SP, Lathro S, Cablanmian A, Kouassi AK, Vergnaud G, Pourcel C - PLoS ONE (2015)

Electron microscopy examination of nine phages representative of the different genera observed in the present phage collection.Scale bar represents 100 nm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482731&req=5

pone.0130548.g012: Electron microscopy examination of nine phages representative of the different genera observed in the present phage collection.Scale bar represents 100 nm.
Mentions: EM examination was performed for at least one phage of each genus (Fig 12). As expected from the genome sequence, phages belonging to the PAK-P1, KPP10 and PB1 genera were myoviruses (A1 morphotype) with long contractile tails. Five PAK-P1-like phages were analysed and all showed the same morphology, with a 130 nm tail and a 67–70 nm head. Ab11, a KPP10-like phage, had a 70 nm head and a 120nm long tail, while virion particles of Ab29, a PB1-like phage, possessed a 74 nm head and a 140 nm long tail. Among podoviruses with short tails (C1 morphotype), Ab05 and Ab22, belonging to the ɸKMV-like and LUZ24-like genera respectively, displayed 60nm icosahedral heads, while Ab09, a N4-like phage, had a 70 nm icosahedral head. Ab18, Ab26 and Ab30 were siphoviruses with a B1 morphotype. Ab18 showed an elongated 85×60nm head and a 130 nm tail similar to that of phage YuA. Ab26 had a head of 60 nm and tail of 170 nm, Ab30 a head of 55 nm and tail of 180 nm.

Bottom Line: The vast majority of the genetic variations among virulent phages from the same genus resulted from seemingly non-random horizontal transfer events, inside a population of P. aeruginosa phages with limited diversity.This is the first study of P. aeruginosa phage diversity in an African city and it shows that a large variety of phage species can be recovered in a limited geographical site at least when different bacterial strains are used.The relative temporal and spatial stability of the Abidjan phage population might reflect equilibrium in the microbial community from which they are released.

View Article: PubMed Central - PubMed

Affiliation: Institute for Integrative Biology of the Cell, CEA, CNRS, Univ Paris-Sud, Université Paris-Saclay, Orsay, France.

ABSTRACT
Twenty two distinct bacteriophages were isolated from sewage water from five locations in the city of Abidjan, Côte d'Ivoire over a two-year period, using a collection of Pseudomonas aeruginosa strains with diverse genotypes. The phages were characterized by their virulence spectrum on a panel of selected P. aeruginosa strains from cystic fibrosis patients and by whole genome sequencing. Twelve virions representing the observed diversity were visualised by electron microscopy. The combined observations showed that 17 phages, distributed into seven genera, were virulent, and that five phages were related to temperate phages belonging to three genera. Some showed similarity with known phages only at the protein level. The vast majority of the genetic variations among virulent phages from the same genus resulted from seemingly non-random horizontal transfer events, inside a population of P. aeruginosa phages with limited diversity. This suggests the existence of a single environmental reservoir or ecotype in which continuous selection is taking place. In contrast, mostly point mutations were observed among phages potentially capable of lysogenisation. This is the first study of P. aeruginosa phage diversity in an African city and it shows that a large variety of phage species can be recovered in a limited geographical site at least when different bacterial strains are used. The relative temporal and spatial stability of the Abidjan phage population might reflect equilibrium in the microbial community from which they are released.

No MeSH data available.


Related in: MedlinePlus