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Isolation and Characterization of a Novel Pathogenesis-Related Protein Gene (GmPRP) with Induced Expression in Soybean (Glycine max) during Infection with Phytophthora sojae.

Jiang L, Wu J, Fan S, Li W, Dong L, Cheng Q, Xu P, Zhang S - PLoS ONE (2015)

Bottom Line: GmPRP was localized in the cell plasma membrane and cytoplasm.Recombinant GmPRP protein exhibited ribonuclease activity and significant inhibition of hyphal growth of P. sojae 1 in vitro.Overexpression of the GmPRP gene in T2 transgenic tobacco and T2 soybean plants resulted in enhanced resistance to Phytophthora nicotianae (P. nicotianae) and P. sojae race 1, respectively.

View Article: PubMed Central - PubMed

Affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.

ABSTRACT
Pathogenesis-related proteins (PR proteins) play crucial roles in the plant defense system. A novel PRP gene was isolated from highly resistant soybean infected with Phytophthora sojae (P. sojae) and was named GmPRP (GenBank accession number: KM506762). The amino acid sequences of GmPRP showed identities of 74%, 73%, 72% and 69% with PRP proteins from Vitis vinifera, Populus trichocarpa, Citrus sinensis and Theobroma cacao, respectively. Quantitative real-time reverse transcription PCR (qRT-PCR) data showed that the expression of GmPRP was highest in roots, followed by the stems and leaves. GmPRP expression was upregulated in soybean leaves infected with P. sojae. Similarly, GmPRP expression also responded to defense/stress signaling molecules, including salicylic acid (SA), ethylene (ET), abscisic acid (ABA) and jasmonic acid (JA). GmPRP was localized in the cell plasma membrane and cytoplasm. Recombinant GmPRP protein exhibited ribonuclease activity and significant inhibition of hyphal growth of P. sojae 1 in vitro. Overexpression of the GmPRP gene in T2 transgenic tobacco and T2 soybean plants resulted in enhanced resistance to Phytophthora nicotianae (P. nicotianae) and P. sojae race 1, respectively. These results indicated that the GmPRP protein played an important role in the defense of soybean against P. sojae infection.

No MeSH data available.


Related in: MedlinePlus

Overexpression of GmPRP in tobacco leaves enhanced the resistance to P. nicotianae.(A) Disease symptoms after infection with P. nicotianae. Row a, tobacco leaves 24 h after inoculation. Row b, tobacco leaves 48 h after inoculation. Row c, tobacco leaves 96 h after inoculation. Column CK, leaves of non-transgenic tobacco. Columns T2-3 and T2-5, leaves of transgenic tobacco. (B) Lesion size of transgenic tobacco leaves infection with P. nicotianae after 96 h. All data represent the mean values of three replications.
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pone.0129932.g007: Overexpression of GmPRP in tobacco leaves enhanced the resistance to P. nicotianae.(A) Disease symptoms after infection with P. nicotianae. Row a, tobacco leaves 24 h after inoculation. Row b, tobacco leaves 48 h after inoculation. Row c, tobacco leaves 96 h after inoculation. Column CK, leaves of non-transgenic tobacco. Columns T2-3 and T2-5, leaves of transgenic tobacco. (B) Lesion size of transgenic tobacco leaves infection with P. nicotianae after 96 h. All data represent the mean values of three replications.

Mentions: GmPRP was overexpressed in tobacco and soybean to evaluate the antimicrobial activity of this protein in vivo. Two transgenic tobacco plants (T2-3 and T2-5) and two transgenic soybean plants (numbered S2-1 and S2-2) were selected to investigate the susceptibility or resistance to P. nicotianae and P. sojae race 1, respectively. After 72 h of incubation with P. nicotianae or P. sojae race 1, remarkable differences in the development of disease symptoms were observed between the transgenic and non-transgenic tobacco and soybean plants. After 72 h of incubation with P. nicotianae, severe symptoms (necrosis and chlorosis) around the infection areas were observed in non-transgenic tobacco plants (Fig 7A, Lane CK), but the transgenic tobacco plants showed only slight lesions (Fig 7A, Lane T2-3, T2-5). The lesion area of the inoculated CK (1.62 cm2) is significantly different from the lesion area of transgenic lines T2-3 and T2-5 (only 1.36 and 0.27 cm2, respectively) (Fig 7B). After 72 h of incubation with P. sojae race 1, the leaves of the non-transgenic soybean plants exhibited clear and large water-soaked lesions compared with those of the transgenic plants (Fig 8A). The lesion area of the inoculated CK (0.34 cm2) is significantly different from the lesion area of transgenic lines T2-3 and T2-5 (only 0.19 and 0.16 cm2, respectively) (Fig 8B)These results indicate that the overexpression of the GmPRP gene in tobacco and soybean plants improved the resistance to P. nicotianae and P. sojae, respectively.


Isolation and Characterization of a Novel Pathogenesis-Related Protein Gene (GmPRP) with Induced Expression in Soybean (Glycine max) during Infection with Phytophthora sojae.

Jiang L, Wu J, Fan S, Li W, Dong L, Cheng Q, Xu P, Zhang S - PLoS ONE (2015)

Overexpression of GmPRP in tobacco leaves enhanced the resistance to P. nicotianae.(A) Disease symptoms after infection with P. nicotianae. Row a, tobacco leaves 24 h after inoculation. Row b, tobacco leaves 48 h after inoculation. Row c, tobacco leaves 96 h after inoculation. Column CK, leaves of non-transgenic tobacco. Columns T2-3 and T2-5, leaves of transgenic tobacco. (B) Lesion size of transgenic tobacco leaves infection with P. nicotianae after 96 h. All data represent the mean values of three replications.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4482714&req=5

pone.0129932.g007: Overexpression of GmPRP in tobacco leaves enhanced the resistance to P. nicotianae.(A) Disease symptoms after infection with P. nicotianae. Row a, tobacco leaves 24 h after inoculation. Row b, tobacco leaves 48 h after inoculation. Row c, tobacco leaves 96 h after inoculation. Column CK, leaves of non-transgenic tobacco. Columns T2-3 and T2-5, leaves of transgenic tobacco. (B) Lesion size of transgenic tobacco leaves infection with P. nicotianae after 96 h. All data represent the mean values of three replications.
Mentions: GmPRP was overexpressed in tobacco and soybean to evaluate the antimicrobial activity of this protein in vivo. Two transgenic tobacco plants (T2-3 and T2-5) and two transgenic soybean plants (numbered S2-1 and S2-2) were selected to investigate the susceptibility or resistance to P. nicotianae and P. sojae race 1, respectively. After 72 h of incubation with P. nicotianae or P. sojae race 1, remarkable differences in the development of disease symptoms were observed between the transgenic and non-transgenic tobacco and soybean plants. After 72 h of incubation with P. nicotianae, severe symptoms (necrosis and chlorosis) around the infection areas were observed in non-transgenic tobacco plants (Fig 7A, Lane CK), but the transgenic tobacco plants showed only slight lesions (Fig 7A, Lane T2-3, T2-5). The lesion area of the inoculated CK (1.62 cm2) is significantly different from the lesion area of transgenic lines T2-3 and T2-5 (only 1.36 and 0.27 cm2, respectively) (Fig 7B). After 72 h of incubation with P. sojae race 1, the leaves of the non-transgenic soybean plants exhibited clear and large water-soaked lesions compared with those of the transgenic plants (Fig 8A). The lesion area of the inoculated CK (0.34 cm2) is significantly different from the lesion area of transgenic lines T2-3 and T2-5 (only 0.19 and 0.16 cm2, respectively) (Fig 8B)These results indicate that the overexpression of the GmPRP gene in tobacco and soybean plants improved the resistance to P. nicotianae and P. sojae, respectively.

Bottom Line: GmPRP was localized in the cell plasma membrane and cytoplasm.Recombinant GmPRP protein exhibited ribonuclease activity and significant inhibition of hyphal growth of P. sojae 1 in vitro.Overexpression of the GmPRP gene in T2 transgenic tobacco and T2 soybean plants resulted in enhanced resistance to Phytophthora nicotianae (P. nicotianae) and P. sojae race 1, respectively.

View Article: PubMed Central - PubMed

Affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.

ABSTRACT
Pathogenesis-related proteins (PR proteins) play crucial roles in the plant defense system. A novel PRP gene was isolated from highly resistant soybean infected with Phytophthora sojae (P. sojae) and was named GmPRP (GenBank accession number: KM506762). The amino acid sequences of GmPRP showed identities of 74%, 73%, 72% and 69% with PRP proteins from Vitis vinifera, Populus trichocarpa, Citrus sinensis and Theobroma cacao, respectively. Quantitative real-time reverse transcription PCR (qRT-PCR) data showed that the expression of GmPRP was highest in roots, followed by the stems and leaves. GmPRP expression was upregulated in soybean leaves infected with P. sojae. Similarly, GmPRP expression also responded to defense/stress signaling molecules, including salicylic acid (SA), ethylene (ET), abscisic acid (ABA) and jasmonic acid (JA). GmPRP was localized in the cell plasma membrane and cytoplasm. Recombinant GmPRP protein exhibited ribonuclease activity and significant inhibition of hyphal growth of P. sojae 1 in vitro. Overexpression of the GmPRP gene in T2 transgenic tobacco and T2 soybean plants resulted in enhanced resistance to Phytophthora nicotianae (P. nicotianae) and P. sojae race 1, respectively. These results indicated that the GmPRP protein played an important role in the defense of soybean against P. sojae infection.

No MeSH data available.


Related in: MedlinePlus