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Isolation and Characterization of a Novel Pathogenesis-Related Protein Gene (GmPRP) with Induced Expression in Soybean (Glycine max) during Infection with Phytophthora sojae.

Jiang L, Wu J, Fan S, Li W, Dong L, Cheng Q, Xu P, Zhang S - PLoS ONE (2015)

Bottom Line: GmPRP was localized in the cell plasma membrane and cytoplasm.Recombinant GmPRP protein exhibited ribonuclease activity and significant inhibition of hyphal growth of P. sojae 1 in vitro.Overexpression of the GmPRP gene in T2 transgenic tobacco and T2 soybean plants resulted in enhanced resistance to Phytophthora nicotianae (P. nicotianae) and P. sojae race 1, respectively.

View Article: PubMed Central - PubMed

Affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.

ABSTRACT
Pathogenesis-related proteins (PR proteins) play crucial roles in the plant defense system. A novel PRP gene was isolated from highly resistant soybean infected with Phytophthora sojae (P. sojae) and was named GmPRP (GenBank accession number: KM506762). The amino acid sequences of GmPRP showed identities of 74%, 73%, 72% and 69% with PRP proteins from Vitis vinifera, Populus trichocarpa, Citrus sinensis and Theobroma cacao, respectively. Quantitative real-time reverse transcription PCR (qRT-PCR) data showed that the expression of GmPRP was highest in roots, followed by the stems and leaves. GmPRP expression was upregulated in soybean leaves infected with P. sojae. Similarly, GmPRP expression also responded to defense/stress signaling molecules, including salicylic acid (SA), ethylene (ET), abscisic acid (ABA) and jasmonic acid (JA). GmPRP was localized in the cell plasma membrane and cytoplasm. Recombinant GmPRP protein exhibited ribonuclease activity and significant inhibition of hyphal growth of P. sojae 1 in vitro. Overexpression of the GmPRP gene in T2 transgenic tobacco and T2 soybean plants resulted in enhanced resistance to Phytophthora nicotianae (P. nicotianae) and P. sojae race 1, respectively. These results indicated that the GmPRP protein played an important role in the defense of soybean against P. sojae infection.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic relationships of GmPRP homologs in plants, constructed using the program MEGA 5.1.
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pone.0129932.g002: Phylogenetic relationships of GmPRP homologs in plants, constructed using the program MEGA 5.1.

Mentions: Sequence comparison showed that the putative GmPRP protein was homologous to PRPs from other plants. Its deduced amino acid sequence showed 74%, 73%, 72% and 69% similarity to Vitis vinifera (XP002262980), Populus trichocarpa (XP002306682), Citrus sinensis (XP006472936) and Theobroma cacao (XP007019416), respectively (Fig 1D). The phylogenetic relationships of GmPRP homologs in plants were constructed using the neighbor-joining method with the program MEGA 5.1. A neighbor-joining (NJ) phylogram was used to construct a phylogenetic tree based on the deduced sequence of GmPRP with other members of the PRP family, indicating that they may share a common ancestor and perform similar functions (Fig 2).


Isolation and Characterization of a Novel Pathogenesis-Related Protein Gene (GmPRP) with Induced Expression in Soybean (Glycine max) during Infection with Phytophthora sojae.

Jiang L, Wu J, Fan S, Li W, Dong L, Cheng Q, Xu P, Zhang S - PLoS ONE (2015)

Phylogenetic relationships of GmPRP homologs in plants, constructed using the program MEGA 5.1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482714&req=5

pone.0129932.g002: Phylogenetic relationships of GmPRP homologs in plants, constructed using the program MEGA 5.1.
Mentions: Sequence comparison showed that the putative GmPRP protein was homologous to PRPs from other plants. Its deduced amino acid sequence showed 74%, 73%, 72% and 69% similarity to Vitis vinifera (XP002262980), Populus trichocarpa (XP002306682), Citrus sinensis (XP006472936) and Theobroma cacao (XP007019416), respectively (Fig 1D). The phylogenetic relationships of GmPRP homologs in plants were constructed using the neighbor-joining method with the program MEGA 5.1. A neighbor-joining (NJ) phylogram was used to construct a phylogenetic tree based on the deduced sequence of GmPRP with other members of the PRP family, indicating that they may share a common ancestor and perform similar functions (Fig 2).

Bottom Line: GmPRP was localized in the cell plasma membrane and cytoplasm.Recombinant GmPRP protein exhibited ribonuclease activity and significant inhibition of hyphal growth of P. sojae 1 in vitro.Overexpression of the GmPRP gene in T2 transgenic tobacco and T2 soybean plants resulted in enhanced resistance to Phytophthora nicotianae (P. nicotianae) and P. sojae race 1, respectively.

View Article: PubMed Central - PubMed

Affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.

ABSTRACT
Pathogenesis-related proteins (PR proteins) play crucial roles in the plant defense system. A novel PRP gene was isolated from highly resistant soybean infected with Phytophthora sojae (P. sojae) and was named GmPRP (GenBank accession number: KM506762). The amino acid sequences of GmPRP showed identities of 74%, 73%, 72% and 69% with PRP proteins from Vitis vinifera, Populus trichocarpa, Citrus sinensis and Theobroma cacao, respectively. Quantitative real-time reverse transcription PCR (qRT-PCR) data showed that the expression of GmPRP was highest in roots, followed by the stems and leaves. GmPRP expression was upregulated in soybean leaves infected with P. sojae. Similarly, GmPRP expression also responded to defense/stress signaling molecules, including salicylic acid (SA), ethylene (ET), abscisic acid (ABA) and jasmonic acid (JA). GmPRP was localized in the cell plasma membrane and cytoplasm. Recombinant GmPRP protein exhibited ribonuclease activity and significant inhibition of hyphal growth of P. sojae 1 in vitro. Overexpression of the GmPRP gene in T2 transgenic tobacco and T2 soybean plants resulted in enhanced resistance to Phytophthora nicotianae (P. nicotianae) and P. sojae race 1, respectively. These results indicated that the GmPRP protein played an important role in the defense of soybean against P. sojae infection.

No MeSH data available.


Related in: MedlinePlus