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The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model.

Song Y, Zheng J, Yan M, Ding W, Xu K, Fan Q, Li Z - PLoS ONE (2015)

Bottom Line: A total of 140 six-month-old male New Zealand rabbits were used.Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

View Article: PubMed Central - PubMed

Affiliation: Orthopedics Oncology Institute of Chinese PLA and Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China; Department of General Surgery, NO. 202 Hospital of PLA, Shenyang, Liaoning, P.R. China.

ABSTRACT

Background: To evaluate the potential effects of irreversible electroporation ablation on the Achilles tendon in a rabbit model and to compare the histopathological and biomechanical changes between specimens following electroporation ablation and radiofrequency ablation.

Methods: A total of 140 six-month-old male New Zealand rabbits were used. The animals were randomly divided into two groups, 70 in the radiofrequency ablation group and 70 in the electroporation group. In situ ablations were applied directly to the Achilles tendons of rabbits using typical electroporation (1800 V/cm, 90 pulses) and radiofrequency ablation (power control mode) protocols. Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.

Results: Both electroporation and radiofrequency ablation produced complete cell ablation in the target region. Thermal damage resulted in tendon rupture 3 days post radiofrequency ablation. In contrast, electroporation-ablated Achilles tendons preserved their biomechanical properties and showed no detectable rupture at this time point. The electroporation-ablated tendons exhibited signs of recovery, including tenoblast regeneration and angiogenesis within 2 weeks, and the restoration of their integral structure was evident within 12 weeks.

Conclusions: When applying electroporation to ablate solid tumors, major advantage could be that collateral damage to adjacent tendons or ligaments is minimized due to the unique ability of electroporation ablation to target the cell membrane. This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

No MeSH data available.


Related in: MedlinePlus

Angiogenesis Analysis.Quantification of the average vascular cross-sectional area in IRE-ablated, distal and proximal regions following sham operations (controls) and IRE ablations. The error bars represent the standard deviation.
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pone.0131404.g010: Angiogenesis Analysis.Quantification of the average vascular cross-sectional area in IRE-ablated, distal and proximal regions following sham operations (controls) and IRE ablations. The error bars represent the standard deviation.

Mentions: We performed this study on the IRE group only. The IOD values of CD31 staining kept higher from 2 w to 12 w than the values observed in controls and peaked at 6 w (Figs 7D and 8). The vascular density decreased at 1 w in the IRE-treated region (Figs 9 and 10), remained at elevated levels from 4 w to 6 w, and ultimately returned to the control levels at 24 w.


The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model.

Song Y, Zheng J, Yan M, Ding W, Xu K, Fan Q, Li Z - PLoS ONE (2015)

Angiogenesis Analysis.Quantification of the average vascular cross-sectional area in IRE-ablated, distal and proximal regions following sham operations (controls) and IRE ablations. The error bars represent the standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482699&req=5

pone.0131404.g010: Angiogenesis Analysis.Quantification of the average vascular cross-sectional area in IRE-ablated, distal and proximal regions following sham operations (controls) and IRE ablations. The error bars represent the standard deviation.
Mentions: We performed this study on the IRE group only. The IOD values of CD31 staining kept higher from 2 w to 12 w than the values observed in controls and peaked at 6 w (Figs 7D and 8). The vascular density decreased at 1 w in the IRE-treated region (Figs 9 and 10), remained at elevated levels from 4 w to 6 w, and ultimately returned to the control levels at 24 w.

Bottom Line: A total of 140 six-month-old male New Zealand rabbits were used.Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

View Article: PubMed Central - PubMed

Affiliation: Orthopedics Oncology Institute of Chinese PLA and Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China; Department of General Surgery, NO. 202 Hospital of PLA, Shenyang, Liaoning, P.R. China.

ABSTRACT

Background: To evaluate the potential effects of irreversible electroporation ablation on the Achilles tendon in a rabbit model and to compare the histopathological and biomechanical changes between specimens following electroporation ablation and radiofrequency ablation.

Methods: A total of 140 six-month-old male New Zealand rabbits were used. The animals were randomly divided into two groups, 70 in the radiofrequency ablation group and 70 in the electroporation group. In situ ablations were applied directly to the Achilles tendons of rabbits using typical electroporation (1800 V/cm, 90 pulses) and radiofrequency ablation (power control mode) protocols. Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.

Results: Both electroporation and radiofrequency ablation produced complete cell ablation in the target region. Thermal damage resulted in tendon rupture 3 days post radiofrequency ablation. In contrast, electroporation-ablated Achilles tendons preserved their biomechanical properties and showed no detectable rupture at this time point. The electroporation-ablated tendons exhibited signs of recovery, including tenoblast regeneration and angiogenesis within 2 weeks, and the restoration of their integral structure was evident within 12 weeks.

Conclusions: When applying electroporation to ablate solid tumors, major advantage could be that collateral damage to adjacent tendons or ligaments is minimized due to the unique ability of electroporation ablation to target the cell membrane. This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

No MeSH data available.


Related in: MedlinePlus