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The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model.

Song Y, Zheng J, Yan M, Ding W, Xu K, Fan Q, Li Z - PLoS ONE (2015)

Bottom Line: A total of 140 six-month-old male New Zealand rabbits were used.Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

View Article: PubMed Central - PubMed

Affiliation: Orthopedics Oncology Institute of Chinese PLA and Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China; Department of General Surgery, NO. 202 Hospital of PLA, Shenyang, Liaoning, P.R. China.

ABSTRACT

Background: To evaluate the potential effects of irreversible electroporation ablation on the Achilles tendon in a rabbit model and to compare the histopathological and biomechanical changes between specimens following electroporation ablation and radiofrequency ablation.

Methods: A total of 140 six-month-old male New Zealand rabbits were used. The animals were randomly divided into two groups, 70 in the radiofrequency ablation group and 70 in the electroporation group. In situ ablations were applied directly to the Achilles tendons of rabbits using typical electroporation (1800 V/cm, 90 pulses) and radiofrequency ablation (power control mode) protocols. Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.

Results: Both electroporation and radiofrequency ablation produced complete cell ablation in the target region. Thermal damage resulted in tendon rupture 3 days post radiofrequency ablation. In contrast, electroporation-ablated Achilles tendons preserved their biomechanical properties and showed no detectable rupture at this time point. The electroporation-ablated tendons exhibited signs of recovery, including tenoblast regeneration and angiogenesis within 2 weeks, and the restoration of their integral structure was evident within 12 weeks.

Conclusions: When applying electroporation to ablate solid tumors, major advantage could be that collateral damage to adjacent tendons or ligaments is minimized due to the unique ability of electroporation ablation to target the cell membrane. This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

No MeSH data available.


Related in: MedlinePlus

Gross specimens of IRE-ablated AT with vascular perfusion at each time point.The blue regions represent the Microfil filling in the blood vessels.
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pone.0131404.g003: Gross specimens of IRE-ablated AT with vascular perfusion at each time point.The blue regions represent the Microfil filling in the blood vessels.

Mentions: In the RFA group, AT rupture occurred at the treated zone approximately 3 d after ablation, on average. The remnants exhibited a gray, jelly-like appearance with blurred ablation boundaries. In addition, it was difficult to distinguish collagen bundles due to widespread coagulation necrosis and the subsequent liquefaction of collagens. In the IRE group, all of the animals were able to move freely, and no detectable ruptures were observed at the time of euthanasia. Compared to controls, hypertrophy of the IRE-ablated region was observed in the specimens from 3 d to 12 w following ablation (Fig 3).


The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model.

Song Y, Zheng J, Yan M, Ding W, Xu K, Fan Q, Li Z - PLoS ONE (2015)

Gross specimens of IRE-ablated AT with vascular perfusion at each time point.The blue regions represent the Microfil filling in the blood vessels.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482699&req=5

pone.0131404.g003: Gross specimens of IRE-ablated AT with vascular perfusion at each time point.The blue regions represent the Microfil filling in the blood vessels.
Mentions: In the RFA group, AT rupture occurred at the treated zone approximately 3 d after ablation, on average. The remnants exhibited a gray, jelly-like appearance with blurred ablation boundaries. In addition, it was difficult to distinguish collagen bundles due to widespread coagulation necrosis and the subsequent liquefaction of collagens. In the IRE group, all of the animals were able to move freely, and no detectable ruptures were observed at the time of euthanasia. Compared to controls, hypertrophy of the IRE-ablated region was observed in the specimens from 3 d to 12 w following ablation (Fig 3).

Bottom Line: A total of 140 six-month-old male New Zealand rabbits were used.Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

View Article: PubMed Central - PubMed

Affiliation: Orthopedics Oncology Institute of Chinese PLA and Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China; Department of General Surgery, NO. 202 Hospital of PLA, Shenyang, Liaoning, P.R. China.

ABSTRACT

Background: To evaluate the potential effects of irreversible electroporation ablation on the Achilles tendon in a rabbit model and to compare the histopathological and biomechanical changes between specimens following electroporation ablation and radiofrequency ablation.

Methods: A total of 140 six-month-old male New Zealand rabbits were used. The animals were randomly divided into two groups, 70 in the radiofrequency ablation group and 70 in the electroporation group. In situ ablations were applied directly to the Achilles tendons of rabbits using typical electroporation (1800 V/cm, 90 pulses) and radiofrequency ablation (power control mode) protocols. Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.

Results: Both electroporation and radiofrequency ablation produced complete cell ablation in the target region. Thermal damage resulted in tendon rupture 3 days post radiofrequency ablation. In contrast, electroporation-ablated Achilles tendons preserved their biomechanical properties and showed no detectable rupture at this time point. The electroporation-ablated tendons exhibited signs of recovery, including tenoblast regeneration and angiogenesis within 2 weeks, and the restoration of their integral structure was evident within 12 weeks.

Conclusions: When applying electroporation to ablate solid tumors, major advantage could be that collateral damage to adjacent tendons or ligaments is minimized due to the unique ability of electroporation ablation to target the cell membrane. This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

No MeSH data available.


Related in: MedlinePlus