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The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model.

Song Y, Zheng J, Yan M, Ding W, Xu K, Fan Q, Li Z - PLoS ONE (2015)

Bottom Line: A total of 140 six-month-old male New Zealand rabbits were used.Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

View Article: PubMed Central - PubMed

Affiliation: Orthopedics Oncology Institute of Chinese PLA and Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China; Department of General Surgery, NO. 202 Hospital of PLA, Shenyang, Liaoning, P.R. China.

ABSTRACT

Background: To evaluate the potential effects of irreversible electroporation ablation on the Achilles tendon in a rabbit model and to compare the histopathological and biomechanical changes between specimens following electroporation ablation and radiofrequency ablation.

Methods: A total of 140 six-month-old male New Zealand rabbits were used. The animals were randomly divided into two groups, 70 in the radiofrequency ablation group and 70 in the electroporation group. In situ ablations were applied directly to the Achilles tendons of rabbits using typical electroporation (1800 V/cm, 90 pulses) and radiofrequency ablation (power control mode) protocols. Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.

Results: Both electroporation and radiofrequency ablation produced complete cell ablation in the target region. Thermal damage resulted in tendon rupture 3 days post radiofrequency ablation. In contrast, electroporation-ablated Achilles tendons preserved their biomechanical properties and showed no detectable rupture at this time point. The electroporation-ablated tendons exhibited signs of recovery, including tenoblast regeneration and angiogenesis within 2 weeks, and the restoration of their integral structure was evident within 12 weeks.

Conclusions: When applying electroporation to ablate solid tumors, major advantage could be that collateral damage to adjacent tendons or ligaments is minimized due to the unique ability of electroporation ablation to target the cell membrane. This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

No MeSH data available.


Related in: MedlinePlus

Surgical procedures and biomechanical tests.(A) Tendon exposure and preparation of the plate electrodes for ablation. (B) The apparatus used to perform the tendon biomechanical tests, including the cryo-jaw, specimens and mechanical testing clamp.
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pone.0131404.g001: Surgical procedures and biomechanical tests.(A) Tendon exposure and preparation of the plate electrodes for ablation. (B) The apparatus used to perform the tendon biomechanical tests, including the cryo-jaw, specimens and mechanical testing clamp.

Mentions: The animals were anesthetized with an intramuscular injection of diazepam (300 μg/kg) and xylazine (5 mg/kg). A segment of tendon at least 20 mm long was prepared for IRE and RFA. A hand-held clamp with two parallel metal electrodes (Tweezertrodes, 45–011, BTX, U.S.) was applied directly to the AT. The distance between the electrodes was measured with a caliper and set at approximately 3.0 mm (Fig 1A). A sequence consisting of 90, 100-microsecond long, direct current square pulses at 1800 V/cm with a pulse interval of 100 milliseconds was applied between the electrodes using an electroporation pulse generator (TP3032, Teslaman, Dalian, China). The power of the RFA was administered using RFA medical equipment (LDRF-120S, Mianyang, China). The initial RFA power was 30 W, and it increased by 10 W every 20 seconds until a sudden and major rise in impedance occurred. Three cycles were performed. The treated length was marked by two knots sutured on the peritendon. During the first 24 hours after surgery, the animals were given two doses of buprenorphine (0.05 mg/kg) and meloxicam (2 mg/kg) at eight-hour intervals. The animals were checked daily to ensure that they recovered, remained healthy, and were not experiencing pain. All tendons were harvested within the first 5 days (d) post-RFA due to AT rupture. Specimens were collected at 3 d, 1 week (w), 2 w, 4 w, 6 w, 12 w and 24 w following IRE.


The Effects of Irreversible Electroporation on the Achilles Tendon: An Experimental Study in a Rabbit Model.

Song Y, Zheng J, Yan M, Ding W, Xu K, Fan Q, Li Z - PLoS ONE (2015)

Surgical procedures and biomechanical tests.(A) Tendon exposure and preparation of the plate electrodes for ablation. (B) The apparatus used to perform the tendon biomechanical tests, including the cryo-jaw, specimens and mechanical testing clamp.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482699&req=5

pone.0131404.g001: Surgical procedures and biomechanical tests.(A) Tendon exposure and preparation of the plate electrodes for ablation. (B) The apparatus used to perform the tendon biomechanical tests, including the cryo-jaw, specimens and mechanical testing clamp.
Mentions: The animals were anesthetized with an intramuscular injection of diazepam (300 μg/kg) and xylazine (5 mg/kg). A segment of tendon at least 20 mm long was prepared for IRE and RFA. A hand-held clamp with two parallel metal electrodes (Tweezertrodes, 45–011, BTX, U.S.) was applied directly to the AT. The distance between the electrodes was measured with a caliper and set at approximately 3.0 mm (Fig 1A). A sequence consisting of 90, 100-microsecond long, direct current square pulses at 1800 V/cm with a pulse interval of 100 milliseconds was applied between the electrodes using an electroporation pulse generator (TP3032, Teslaman, Dalian, China). The power of the RFA was administered using RFA medical equipment (LDRF-120S, Mianyang, China). The initial RFA power was 30 W, and it increased by 10 W every 20 seconds until a sudden and major rise in impedance occurred. Three cycles were performed. The treated length was marked by two knots sutured on the peritendon. During the first 24 hours after surgery, the animals were given two doses of buprenorphine (0.05 mg/kg) and meloxicam (2 mg/kg) at eight-hour intervals. The animals were checked daily to ensure that they recovered, remained healthy, and were not experiencing pain. All tendons were harvested within the first 5 days (d) post-RFA due to AT rupture. Specimens were collected at 3 d, 1 week (w), 2 w, 4 w, 6 w, 12 w and 24 w following IRE.

Bottom Line: A total of 140 six-month-old male New Zealand rabbits were used.Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

View Article: PubMed Central - PubMed

Affiliation: Orthopedics Oncology Institute of Chinese PLA and Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China; Department of General Surgery, NO. 202 Hospital of PLA, Shenyang, Liaoning, P.R. China.

ABSTRACT

Background: To evaluate the potential effects of irreversible electroporation ablation on the Achilles tendon in a rabbit model and to compare the histopathological and biomechanical changes between specimens following electroporation ablation and radiofrequency ablation.

Methods: A total of 140 six-month-old male New Zealand rabbits were used. The animals were randomly divided into two groups, 70 in the radiofrequency ablation group and 70 in the electroporation group. In situ ablations were applied directly to the Achilles tendons of rabbits using typical electroporation (1800 V/cm, 90 pulses) and radiofrequency ablation (power control mode) protocols. Histopathological and biomechanical evaluations were performed to examine the effects of electroporation ablation and radiofrequency ablation over time.

Results: Both electroporation and radiofrequency ablation produced complete cell ablation in the target region. Thermal damage resulted in tendon rupture 3 days post radiofrequency ablation. In contrast, electroporation-ablated Achilles tendons preserved their biomechanical properties and showed no detectable rupture at this time point. The electroporation-ablated tendons exhibited signs of recovery, including tenoblast regeneration and angiogenesis within 2 weeks, and the restoration of their integral structure was evident within 12 weeks.

Conclusions: When applying electroporation to ablate solid tumors, major advantage could be that collateral damage to adjacent tendons or ligaments is minimized due to the unique ability of electroporation ablation to target the cell membrane. This advantage could have a significant impact on the field of tumor ablation near vital tendons or ligaments.

No MeSH data available.


Related in: MedlinePlus