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Global Transcriptome Profiles of 'Meyer' Zoysiagrass in Response to Cold Stress.

Wei S, Du Z, Gao F, Ke X, Li J, Liu J, Zhou Y - PLoS ONE (2015)

Bottom Line: Zoysiagrasses (Zoysia spp.In response to the stress, genes involved in proline synthesis, in starch hydrolysis, in methionine and ascorbic acid metabolism, in SOD activity, and in DREBs response pathway were up-regulated.GA metabolism, ABA and JA stimulus response were affected under cold exposure.

View Article: PubMed Central - PubMed

Affiliation: College of Life & Environmental Sciences, Minzu University of China, Beijing, PR China.

ABSTRACT
A long green period is essential for a turfgrass species with high ornamental value and a wide area of use. Zoysiagrasses (Zoysia spp. Willd.) are perennial turfgrass species popular in tropical, subtropical and temperate zones, possessing many properties necessary to be economically useful turfgrass. They do not have a long green period because of cold sensitivity. A main focus in zoysiagrass research is to develop cold tolerant cultivars. Understanding the cold response in zoysiagrass is a fundamental area of research. In the present study, 'Meyer' zoysiagrass (Zoysia japonica), a widely cultivated variety in the genus, is used. We employed RNA-Seq to investigate genome-wide gene expression profiles in leaves under cold stress (4°C). Using the Illumina sequencing platform, we obtained approximately 206 million high-quality paired-end reads from three libraries (0 h, 2 h, and 72 h cold treatment at 4°C). After de novo assembly and quantitative assessment, 46,412 unigenes were generated with an average length of 998 bp and an N50 of 1,522 bp. A total of 25,644 (55.2%) unigenes were annotated by alignment with public protein databases including NR, SwissProt, KEGG and KOG. Differentially expressed genes (DEGs) were investigated using the RPKM method. A total of 756 DEGs were identified between 0 h and 2 h-cold treatment, with 522 up-regulated and 234 down-regulated; and 5327 DEGs were identified between 0 h and 72 h-cold treatment, with 2453 up-regulated and 2874 down-regulated. The expression profile of 15 DEGs selected randomly was confirmed with qRT-PCR. The results suggest that cold stress can induce desiccation and oxidative stress, inhibit photosynthesis and substance transport. In response to the stress, genes involved in proline synthesis, in starch hydrolysis, in methionine and ascorbic acid metabolism, in SOD activity, and in DREBs response pathway were up-regulated. GA metabolism, ABA and JA stimulus response were affected under cold exposure. This is the first transcriptome sequencing of Z. japonica, providing a large set of sequence data as well as gene expression profiles under cold stress. It will improve our current understanding of the cold response of zoysiagrass and be beneficial in breeding research.

No MeSH data available.


Related in: MedlinePlus

Chlorophyll metabolism in ‘Meyer’ under cold stress.gltX, glutamyl-tRNA synthetase; GluTR, Glutamyl-tRNA reductase; PPOX, protoporphyrinogen oxidase; Chl H, magnesium chelatase; EC 2.1.1.11, magnesium protoporphyrin IX methyltransferase; EC 1.14.13.81, magnesium-protoporphyrin IX monomethy lester (oxidative) cyclase; EC 1.3.1.33, protochlorophyllide reductase; EC 3.1.1.14, chlorophyllase; PAO, Pheophorbide a oxygenase; Hem H, ferrochelatase; COX15, cytochrome c oxidase 15 (Heme A synthase). In brackets, red numbers represent the number of up-regulated unigenes, blue numbers represent the number of down-regulated unigenes.
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pone.0131153.g008: Chlorophyll metabolism in ‘Meyer’ under cold stress.gltX, glutamyl-tRNA synthetase; GluTR, Glutamyl-tRNA reductase; PPOX, protoporphyrinogen oxidase; Chl H, magnesium chelatase; EC 2.1.1.11, magnesium protoporphyrin IX methyltransferase; EC 1.14.13.81, magnesium-protoporphyrin IX monomethy lester (oxidative) cyclase; EC 1.3.1.33, protochlorophyllide reductase; EC 3.1.1.14, chlorophyllase; PAO, Pheophorbide a oxygenase; Hem H, ferrochelatase; COX15, cytochrome c oxidase 15 (Heme A synthase). In brackets, red numbers represent the number of up-regulated unigenes, blue numbers represent the number of down-regulated unigenes.

Mentions: Chlorophylls are complex molecules exquisitely suited to the light absorption, energy transfer, and electron transfer functions carried out in photosynthesis. During the 72h-4°C stress, most of the DEGs involved in chlorophyll synthesis were down-regulated (Fig 8). At the same time, one unigene of ferrochelatase (Hem H), and one of pheophorbide a oxygenase (PAO) were up-regulated. Hem H catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme, which departs from the chlorophyll biosynthetic pathway. PAO, the key enzyme in chlorophyll catabolism, catalyzes porphyrinmacrocycle cleavage of pheophorbide a (pheide a) to a primary fluorescent catabolite (pFCC) [30]; its expression was up-regulated during senescence [31]. Taken together, these gene expression changes suggest that chlorophyll biosynthesis decreases and chlorophyll catabolism increases during low temperature treatment.


Global Transcriptome Profiles of 'Meyer' Zoysiagrass in Response to Cold Stress.

Wei S, Du Z, Gao F, Ke X, Li J, Liu J, Zhou Y - PLoS ONE (2015)

Chlorophyll metabolism in ‘Meyer’ under cold stress.gltX, glutamyl-tRNA synthetase; GluTR, Glutamyl-tRNA reductase; PPOX, protoporphyrinogen oxidase; Chl H, magnesium chelatase; EC 2.1.1.11, magnesium protoporphyrin IX methyltransferase; EC 1.14.13.81, magnesium-protoporphyrin IX monomethy lester (oxidative) cyclase; EC 1.3.1.33, protochlorophyllide reductase; EC 3.1.1.14, chlorophyllase; PAO, Pheophorbide a oxygenase; Hem H, ferrochelatase; COX15, cytochrome c oxidase 15 (Heme A synthase). In brackets, red numbers represent the number of up-regulated unigenes, blue numbers represent the number of down-regulated unigenes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482698&req=5

pone.0131153.g008: Chlorophyll metabolism in ‘Meyer’ under cold stress.gltX, glutamyl-tRNA synthetase; GluTR, Glutamyl-tRNA reductase; PPOX, protoporphyrinogen oxidase; Chl H, magnesium chelatase; EC 2.1.1.11, magnesium protoporphyrin IX methyltransferase; EC 1.14.13.81, magnesium-protoporphyrin IX monomethy lester (oxidative) cyclase; EC 1.3.1.33, protochlorophyllide reductase; EC 3.1.1.14, chlorophyllase; PAO, Pheophorbide a oxygenase; Hem H, ferrochelatase; COX15, cytochrome c oxidase 15 (Heme A synthase). In brackets, red numbers represent the number of up-regulated unigenes, blue numbers represent the number of down-regulated unigenes.
Mentions: Chlorophylls are complex molecules exquisitely suited to the light absorption, energy transfer, and electron transfer functions carried out in photosynthesis. During the 72h-4°C stress, most of the DEGs involved in chlorophyll synthesis were down-regulated (Fig 8). At the same time, one unigene of ferrochelatase (Hem H), and one of pheophorbide a oxygenase (PAO) were up-regulated. Hem H catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme, which departs from the chlorophyll biosynthetic pathway. PAO, the key enzyme in chlorophyll catabolism, catalyzes porphyrinmacrocycle cleavage of pheophorbide a (pheide a) to a primary fluorescent catabolite (pFCC) [30]; its expression was up-regulated during senescence [31]. Taken together, these gene expression changes suggest that chlorophyll biosynthesis decreases and chlorophyll catabolism increases during low temperature treatment.

Bottom Line: Zoysiagrasses (Zoysia spp.In response to the stress, genes involved in proline synthesis, in starch hydrolysis, in methionine and ascorbic acid metabolism, in SOD activity, and in DREBs response pathway were up-regulated.GA metabolism, ABA and JA stimulus response were affected under cold exposure.

View Article: PubMed Central - PubMed

Affiliation: College of Life & Environmental Sciences, Minzu University of China, Beijing, PR China.

ABSTRACT
A long green period is essential for a turfgrass species with high ornamental value and a wide area of use. Zoysiagrasses (Zoysia spp. Willd.) are perennial turfgrass species popular in tropical, subtropical and temperate zones, possessing many properties necessary to be economically useful turfgrass. They do not have a long green period because of cold sensitivity. A main focus in zoysiagrass research is to develop cold tolerant cultivars. Understanding the cold response in zoysiagrass is a fundamental area of research. In the present study, 'Meyer' zoysiagrass (Zoysia japonica), a widely cultivated variety in the genus, is used. We employed RNA-Seq to investigate genome-wide gene expression profiles in leaves under cold stress (4°C). Using the Illumina sequencing platform, we obtained approximately 206 million high-quality paired-end reads from three libraries (0 h, 2 h, and 72 h cold treatment at 4°C). After de novo assembly and quantitative assessment, 46,412 unigenes were generated with an average length of 998 bp and an N50 of 1,522 bp. A total of 25,644 (55.2%) unigenes were annotated by alignment with public protein databases including NR, SwissProt, KEGG and KOG. Differentially expressed genes (DEGs) were investigated using the RPKM method. A total of 756 DEGs were identified between 0 h and 2 h-cold treatment, with 522 up-regulated and 234 down-regulated; and 5327 DEGs were identified between 0 h and 72 h-cold treatment, with 2453 up-regulated and 2874 down-regulated. The expression profile of 15 DEGs selected randomly was confirmed with qRT-PCR. The results suggest that cold stress can induce desiccation and oxidative stress, inhibit photosynthesis and substance transport. In response to the stress, genes involved in proline synthesis, in starch hydrolysis, in methionine and ascorbic acid metabolism, in SOD activity, and in DREBs response pathway were up-regulated. GA metabolism, ABA and JA stimulus response were affected under cold exposure. This is the first transcriptome sequencing of Z. japonica, providing a large set of sequence data as well as gene expression profiles under cold stress. It will improve our current understanding of the cold response of zoysiagrass and be beneficial in breeding research.

No MeSH data available.


Related in: MedlinePlus