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Global Transcriptome Profiles of 'Meyer' Zoysiagrass in Response to Cold Stress.

Wei S, Du Z, Gao F, Ke X, Li J, Liu J, Zhou Y - PLoS ONE (2015)

Bottom Line: Zoysiagrasses (Zoysia spp.In response to the stress, genes involved in proline synthesis, in starch hydrolysis, in methionine and ascorbic acid metabolism, in SOD activity, and in DREBs response pathway were up-regulated.GA metabolism, ABA and JA stimulus response were affected under cold exposure.

View Article: PubMed Central - PubMed

Affiliation: College of Life & Environmental Sciences, Minzu University of China, Beijing, PR China.

ABSTRACT
A long green period is essential for a turfgrass species with high ornamental value and a wide area of use. Zoysiagrasses (Zoysia spp. Willd.) are perennial turfgrass species popular in tropical, subtropical and temperate zones, possessing many properties necessary to be economically useful turfgrass. They do not have a long green period because of cold sensitivity. A main focus in zoysiagrass research is to develop cold tolerant cultivars. Understanding the cold response in zoysiagrass is a fundamental area of research. In the present study, 'Meyer' zoysiagrass (Zoysia japonica), a widely cultivated variety in the genus, is used. We employed RNA-Seq to investigate genome-wide gene expression profiles in leaves under cold stress (4°C). Using the Illumina sequencing platform, we obtained approximately 206 million high-quality paired-end reads from three libraries (0 h, 2 h, and 72 h cold treatment at 4°C). After de novo assembly and quantitative assessment, 46,412 unigenes were generated with an average length of 998 bp and an N50 of 1,522 bp. A total of 25,644 (55.2%) unigenes were annotated by alignment with public protein databases including NR, SwissProt, KEGG and KOG. Differentially expressed genes (DEGs) were investigated using the RPKM method. A total of 756 DEGs were identified between 0 h and 2 h-cold treatment, with 522 up-regulated and 234 down-regulated; and 5327 DEGs were identified between 0 h and 72 h-cold treatment, with 2453 up-regulated and 2874 down-regulated. The expression profile of 15 DEGs selected randomly was confirmed with qRT-PCR. The results suggest that cold stress can induce desiccation and oxidative stress, inhibit photosynthesis and substance transport. In response to the stress, genes involved in proline synthesis, in starch hydrolysis, in methionine and ascorbic acid metabolism, in SOD activity, and in DREBs response pathway were up-regulated. GA metabolism, ABA and JA stimulus response were affected under cold exposure. This is the first transcriptome sequencing of Z. japonica, providing a large set of sequence data as well as gene expression profiles under cold stress. It will improve our current understanding of the cold response of zoysiagrass and be beneficial in breeding research.

No MeSH data available.


Related in: MedlinePlus

Physiological change in leaves under cold stress.‘Meyer’ was placed in growth chambers at 4°C for the cold treatment and at 25°C for the control. The second expanded leaf from the top was used for determinations. Experiments were conducted with five biological replicates, and results were expressed as mean ± standard error (SE). *P < 0.05.
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pone.0131153.g001: Physiological change in leaves under cold stress.‘Meyer’ was placed in growth chambers at 4°C for the cold treatment and at 25°C for the control. The second expanded leaf from the top was used for determinations. Experiments were conducted with five biological replicates, and results were expressed as mean ± standard error (SE). *P < 0.05.

Mentions: To investigate physiological responses in leaves, we measured relative water content (RWC) and levels of total soluble carbohydrate, proline and malondialdehyde (MDA). The activities of antioxidant enzymes, including SOD, CAT and POD, were also determined. After 2h at 4°C, RWC declined significantly, and the content of total soluble carbohydrate increased (P < 0.05). After 72h at 4°C, RWC remained constant, whereas soluble carbohydrate, proline and MDA increased (P < 0.05) considerably (Fig 1A–1D). The activities of SOD and CAT in leaves exposed to 2h did not change significantly (P ≥ 0.05), while the activity of POD increased by 14.67% (P < 0.05). After a 72h-cold exposure, SOD activity increased by 10.78%, while activities of CAT and POD decreased by 35.99% (P <0.05) and 13.85% (P <0.05), respectively (Fig 1E–1G).


Global Transcriptome Profiles of 'Meyer' Zoysiagrass in Response to Cold Stress.

Wei S, Du Z, Gao F, Ke X, Li J, Liu J, Zhou Y - PLoS ONE (2015)

Physiological change in leaves under cold stress.‘Meyer’ was placed in growth chambers at 4°C for the cold treatment and at 25°C for the control. The second expanded leaf from the top was used for determinations. Experiments were conducted with five biological replicates, and results were expressed as mean ± standard error (SE). *P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482698&req=5

pone.0131153.g001: Physiological change in leaves under cold stress.‘Meyer’ was placed in growth chambers at 4°C for the cold treatment and at 25°C for the control. The second expanded leaf from the top was used for determinations. Experiments were conducted with five biological replicates, and results were expressed as mean ± standard error (SE). *P < 0.05.
Mentions: To investigate physiological responses in leaves, we measured relative water content (RWC) and levels of total soluble carbohydrate, proline and malondialdehyde (MDA). The activities of antioxidant enzymes, including SOD, CAT and POD, were also determined. After 2h at 4°C, RWC declined significantly, and the content of total soluble carbohydrate increased (P < 0.05). After 72h at 4°C, RWC remained constant, whereas soluble carbohydrate, proline and MDA increased (P < 0.05) considerably (Fig 1A–1D). The activities of SOD and CAT in leaves exposed to 2h did not change significantly (P ≥ 0.05), while the activity of POD increased by 14.67% (P < 0.05). After a 72h-cold exposure, SOD activity increased by 10.78%, while activities of CAT and POD decreased by 35.99% (P <0.05) and 13.85% (P <0.05), respectively (Fig 1E–1G).

Bottom Line: Zoysiagrasses (Zoysia spp.In response to the stress, genes involved in proline synthesis, in starch hydrolysis, in methionine and ascorbic acid metabolism, in SOD activity, and in DREBs response pathway were up-regulated.GA metabolism, ABA and JA stimulus response were affected under cold exposure.

View Article: PubMed Central - PubMed

Affiliation: College of Life & Environmental Sciences, Minzu University of China, Beijing, PR China.

ABSTRACT
A long green period is essential for a turfgrass species with high ornamental value and a wide area of use. Zoysiagrasses (Zoysia spp. Willd.) are perennial turfgrass species popular in tropical, subtropical and temperate zones, possessing many properties necessary to be economically useful turfgrass. They do not have a long green period because of cold sensitivity. A main focus in zoysiagrass research is to develop cold tolerant cultivars. Understanding the cold response in zoysiagrass is a fundamental area of research. In the present study, 'Meyer' zoysiagrass (Zoysia japonica), a widely cultivated variety in the genus, is used. We employed RNA-Seq to investigate genome-wide gene expression profiles in leaves under cold stress (4°C). Using the Illumina sequencing platform, we obtained approximately 206 million high-quality paired-end reads from three libraries (0 h, 2 h, and 72 h cold treatment at 4°C). After de novo assembly and quantitative assessment, 46,412 unigenes were generated with an average length of 998 bp and an N50 of 1,522 bp. A total of 25,644 (55.2%) unigenes were annotated by alignment with public protein databases including NR, SwissProt, KEGG and KOG. Differentially expressed genes (DEGs) were investigated using the RPKM method. A total of 756 DEGs were identified between 0 h and 2 h-cold treatment, with 522 up-regulated and 234 down-regulated; and 5327 DEGs were identified between 0 h and 72 h-cold treatment, with 2453 up-regulated and 2874 down-regulated. The expression profile of 15 DEGs selected randomly was confirmed with qRT-PCR. The results suggest that cold stress can induce desiccation and oxidative stress, inhibit photosynthesis and substance transport. In response to the stress, genes involved in proline synthesis, in starch hydrolysis, in methionine and ascorbic acid metabolism, in SOD activity, and in DREBs response pathway were up-regulated. GA metabolism, ABA and JA stimulus response were affected under cold exposure. This is the first transcriptome sequencing of Z. japonica, providing a large set of sequence data as well as gene expression profiles under cold stress. It will improve our current understanding of the cold response of zoysiagrass and be beneficial in breeding research.

No MeSH data available.


Related in: MedlinePlus