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A Comparative Transcriptome Analysis between Wild and Albino Yellow Catfish (Pelteobagrus fulvidraco).

Zou M, Zhang X, Shi Z, Lin L, Ouyang G, Zhang G, Zheng H, Wei K, Ji W - PLoS ONE (2015)

Bottom Line: However, non-synonymous mutations result from single nucleotide substitutions residing in coding regions may not contribute to such differences.Based on the high-throughput expression data generated for the two different types of P. fulvidraco, we found that alterations of expression pattern may be more common than non-synonymous mutations.The transcriptome of P. fulvidraco will be an invaluable resource for subsequent comparative genomics and evolutionary analyses of this economically important fish.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Freshwater Aquaculture Collaborative Innovation Centre of Hubei Province, Wuhan, Hubei, 430070, China.

ABSTRACT
Body colours are important and striking features for individual survival and reproductive success, in particular in vertebrates where mating behaviour and mate preference may be strongly influenced by non-normal phenotypes. Pigmentation disorders may be generated by disruption of one or many independent genes as well as by environmental factors. The first discovery of albino yellow catfish (Pelteobagrus fulvidraco Richardson) with golden skin colour from fish farms in China provides us valuable material to study the molecular mechanism underlying the abnormalities of pigmentation. In this study, transcriptome sequencing of fin tissues corresponding to the distinct body colours, wild type and mutant albino yellow catfish, were performed using Illumina sequencing technology. Based on next-generation sequencing technology and de novo assembly, we generated a transcriptome of P. fulvidraco. A number of genes differentially expressed between the wild types and albinos were identified, suggesting their contribution to the different phenotypes and fitness. However, non-synonymous mutations result from single nucleotide substitutions residing in coding regions may not contribute to such differences. Based on the high-throughput expression data generated for the two different types of P. fulvidraco, we found that alterations of expression pattern may be more common than non-synonymous mutations. The transcriptome of P. fulvidraco will be an invaluable resource for subsequent comparative genomics and evolutionary analyses of this economically important fish.

No MeSH data available.


Related in: MedlinePlus

The results of qRT-PCR for the DEs identified by RNA-Seq and subject to validation.
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pone.0131504.g004: The results of qRT-PCR for the DEs identified by RNA-Seq and subject to validation.

Mentions: To further validate the DE genes detected by RNA-Seq, another sixteen candidates (twelve were from Table 4 and another 4 were randomly selected from S2 Table) were selected and qRT-PCR amplification confirmed the expression patterns of 13 genes. For the other three DE candidates (lipoprotein receptor, Utrophin and Ptprc), no significant difference was detected (Fig 4).


A Comparative Transcriptome Analysis between Wild and Albino Yellow Catfish (Pelteobagrus fulvidraco).

Zou M, Zhang X, Shi Z, Lin L, Ouyang G, Zhang G, Zheng H, Wei K, Ji W - PLoS ONE (2015)

The results of qRT-PCR for the DEs identified by RNA-Seq and subject to validation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482592&req=5

pone.0131504.g004: The results of qRT-PCR for the DEs identified by RNA-Seq and subject to validation.
Mentions: To further validate the DE genes detected by RNA-Seq, another sixteen candidates (twelve were from Table 4 and another 4 were randomly selected from S2 Table) were selected and qRT-PCR amplification confirmed the expression patterns of 13 genes. For the other three DE candidates (lipoprotein receptor, Utrophin and Ptprc), no significant difference was detected (Fig 4).

Bottom Line: However, non-synonymous mutations result from single nucleotide substitutions residing in coding regions may not contribute to such differences.Based on the high-throughput expression data generated for the two different types of P. fulvidraco, we found that alterations of expression pattern may be more common than non-synonymous mutations.The transcriptome of P. fulvidraco will be an invaluable resource for subsequent comparative genomics and evolutionary analyses of this economically important fish.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Freshwater Aquaculture Collaborative Innovation Centre of Hubei Province, Wuhan, Hubei, 430070, China.

ABSTRACT
Body colours are important and striking features for individual survival and reproductive success, in particular in vertebrates where mating behaviour and mate preference may be strongly influenced by non-normal phenotypes. Pigmentation disorders may be generated by disruption of one or many independent genes as well as by environmental factors. The first discovery of albino yellow catfish (Pelteobagrus fulvidraco Richardson) with golden skin colour from fish farms in China provides us valuable material to study the molecular mechanism underlying the abnormalities of pigmentation. In this study, transcriptome sequencing of fin tissues corresponding to the distinct body colours, wild type and mutant albino yellow catfish, were performed using Illumina sequencing technology. Based on next-generation sequencing technology and de novo assembly, we generated a transcriptome of P. fulvidraco. A number of genes differentially expressed between the wild types and albinos were identified, suggesting their contribution to the different phenotypes and fitness. However, non-synonymous mutations result from single nucleotide substitutions residing in coding regions may not contribute to such differences. Based on the high-throughput expression data generated for the two different types of P. fulvidraco, we found that alterations of expression pattern may be more common than non-synonymous mutations. The transcriptome of P. fulvidraco will be an invaluable resource for subsequent comparative genomics and evolutionary analyses of this economically important fish.

No MeSH data available.


Related in: MedlinePlus