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A Comparative Transcriptome Analysis between Wild and Albino Yellow Catfish (Pelteobagrus fulvidraco).

Zou M, Zhang X, Shi Z, Lin L, Ouyang G, Zhang G, Zheng H, Wei K, Ji W - PLoS ONE (2015)

Bottom Line: However, non-synonymous mutations result from single nucleotide substitutions residing in coding regions may not contribute to such differences.Based on the high-throughput expression data generated for the two different types of P. fulvidraco, we found that alterations of expression pattern may be more common than non-synonymous mutations.The transcriptome of P. fulvidraco will be an invaluable resource for subsequent comparative genomics and evolutionary analyses of this economically important fish.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Freshwater Aquaculture Collaborative Innovation Centre of Hubei Province, Wuhan, Hubei, 430070, China.

ABSTRACT
Body colours are important and striking features for individual survival and reproductive success, in particular in vertebrates where mating behaviour and mate preference may be strongly influenced by non-normal phenotypes. Pigmentation disorders may be generated by disruption of one or many independent genes as well as by environmental factors. The first discovery of albino yellow catfish (Pelteobagrus fulvidraco Richardson) with golden skin colour from fish farms in China provides us valuable material to study the molecular mechanism underlying the abnormalities of pigmentation. In this study, transcriptome sequencing of fin tissues corresponding to the distinct body colours, wild type and mutant albino yellow catfish, were performed using Illumina sequencing technology. Based on next-generation sequencing technology and de novo assembly, we generated a transcriptome of P. fulvidraco. A number of genes differentially expressed between the wild types and albinos were identified, suggesting their contribution to the different phenotypes and fitness. However, non-synonymous mutations result from single nucleotide substitutions residing in coding regions may not contribute to such differences. Based on the high-throughput expression data generated for the two different types of P. fulvidraco, we found that alterations of expression pattern may be more common than non-synonymous mutations. The transcriptome of P. fulvidraco will be an invaluable resource for subsequent comparative genomics and evolutionary analyses of this economically important fish.

No MeSH data available.


Related in: MedlinePlus

Wild and albino yellow catfish.
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pone.0131504.g001: Wild and albino yellow catfish.

Mentions: Yellow catfish, a teleost belonging to the family Bagridae, is one of the most important economic freshwater species in China due to its excellent meat quality. This species is extensively cultured in East and South Asia in semi-intensive and intensive systems [29]. In 2012, total production of yellow catfish reached 256,650 tonnes in China [30]. During the breeding of yellow catfish, we found a small number of fish with golden-pink eyes and golden colour all over the body (Fig 1). We consider this colour variation to result from an absence of melanin pigmentation, just like albinism reported in other species. Given the complexity of colour variation and albinism, an understanding of how melanin pigmentation is regulated has a long way to go. Transcriptome analysis of yellow catfish with golden colour will help us to better understand the mechanism for pigment biosynthesis. In this study, we sequenced the transcriptome of the normal and the newly discovered albino yellow catfish strain (thereafter called wild type and albino type, respectively) using Illumina sequencing technology. We report the differentially expressed genes (DEs) and mutations that may contribute to the phenotypic differences between these two strains, which may provide genetic information for fish colour variation.


A Comparative Transcriptome Analysis between Wild and Albino Yellow Catfish (Pelteobagrus fulvidraco).

Zou M, Zhang X, Shi Z, Lin L, Ouyang G, Zhang G, Zheng H, Wei K, Ji W - PLoS ONE (2015)

Wild and albino yellow catfish.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482592&req=5

pone.0131504.g001: Wild and albino yellow catfish.
Mentions: Yellow catfish, a teleost belonging to the family Bagridae, is one of the most important economic freshwater species in China due to its excellent meat quality. This species is extensively cultured in East and South Asia in semi-intensive and intensive systems [29]. In 2012, total production of yellow catfish reached 256,650 tonnes in China [30]. During the breeding of yellow catfish, we found a small number of fish with golden-pink eyes and golden colour all over the body (Fig 1). We consider this colour variation to result from an absence of melanin pigmentation, just like albinism reported in other species. Given the complexity of colour variation and albinism, an understanding of how melanin pigmentation is regulated has a long way to go. Transcriptome analysis of yellow catfish with golden colour will help us to better understand the mechanism for pigment biosynthesis. In this study, we sequenced the transcriptome of the normal and the newly discovered albino yellow catfish strain (thereafter called wild type and albino type, respectively) using Illumina sequencing technology. We report the differentially expressed genes (DEs) and mutations that may contribute to the phenotypic differences between these two strains, which may provide genetic information for fish colour variation.

Bottom Line: However, non-synonymous mutations result from single nucleotide substitutions residing in coding regions may not contribute to such differences.Based on the high-throughput expression data generated for the two different types of P. fulvidraco, we found that alterations of expression pattern may be more common than non-synonymous mutations.The transcriptome of P. fulvidraco will be an invaluable resource for subsequent comparative genomics and evolutionary analyses of this economically important fish.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Freshwater Aquaculture Collaborative Innovation Centre of Hubei Province, Wuhan, Hubei, 430070, China.

ABSTRACT
Body colours are important and striking features for individual survival and reproductive success, in particular in vertebrates where mating behaviour and mate preference may be strongly influenced by non-normal phenotypes. Pigmentation disorders may be generated by disruption of one or many independent genes as well as by environmental factors. The first discovery of albino yellow catfish (Pelteobagrus fulvidraco Richardson) with golden skin colour from fish farms in China provides us valuable material to study the molecular mechanism underlying the abnormalities of pigmentation. In this study, transcriptome sequencing of fin tissues corresponding to the distinct body colours, wild type and mutant albino yellow catfish, were performed using Illumina sequencing technology. Based on next-generation sequencing technology and de novo assembly, we generated a transcriptome of P. fulvidraco. A number of genes differentially expressed between the wild types and albinos were identified, suggesting their contribution to the different phenotypes and fitness. However, non-synonymous mutations result from single nucleotide substitutions residing in coding regions may not contribute to such differences. Based on the high-throughput expression data generated for the two different types of P. fulvidraco, we found that alterations of expression pattern may be more common than non-synonymous mutations. The transcriptome of P. fulvidraco will be an invaluable resource for subsequent comparative genomics and evolutionary analyses of this economically important fish.

No MeSH data available.


Related in: MedlinePlus