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Canine Antibodies against Salivary Recombinant Proteins of Phlebotomus perniciosus: A Longitudinal Study in an Endemic Focus of Canine Leishmaniasis.

Kostalova T, Lestinova T, Sumova P, Vlkova M, Rohousova I, Berriatua E, Oliva G, Fiorentino E, Scalone A, Gramiccia M, Gradoni L, Volf P - PLoS Negl Trop Dis (2015)

Bottom Line: We found a significant association between active CanL infection and the amount of anti-P. perniciosus saliva antibodies.These results suggest that P. perniciosus rSP03B protein is a valid alternative to whole saliva and could be used in large-scale serological studies.This novel method could be a practical and economically-sound tool to detect the host exposure to sand fly bites in CanL endemic areas.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.

ABSTRACT

Background: Phlebotomine sand flies are vectors of Leishmania parasites. During blood feeding, sand flies deposit into the host skin immunogenic salivary proteins which elicit specific antibody responses. These anti-saliva antibodies enable an estimate of the host exposure to sand flies and, in leishmaniasis endemic areas, also the risk for Leishmania infections. However, the use of whole salivary gland homogenates as antigen has several limitations, and therefore, recombinant salivary proteins have been tested to replace them in antibody detection assays. In this study, we have used for the first time sand fly salivary recombinant proteins in a longitudinal field study on dogs.

Methodology/principal findings: Sera from dogs naturally exposed to P. perniciosus bites over two consecutive transmission seasons in a site endemic for canine leishmaniasis (CanL) were tested at different time points by ELISA for the antibodies recognizing whole saliva, single salivary 43 kDa yellow-related recombinant protein (rSP03B), and a combination of two salivary recombinant proteins, 43 kDa yellow-related protein and 35.5 kDa apyrase (rSP01). Dogs were also tested for Leishmania infantum positivity by serology, culture, and PCR and the infection status was evaluated prospectively. We found a significant association between active CanL infection and the amount of anti-P. perniciosus saliva antibodies. Importantly, we detected a high correlation between IgG antibodies recognizing rSP03B protein and the whole salivary antigen. The kinetics of antibody response showed for both a whole saliva and rSP03B a similar pattern that was clearly related to the seasonal abundance of P. perniciosus.

Conclusions: These results suggest that P. perniciosus rSP03B protein is a valid alternative to whole saliva and could be used in large-scale serological studies. This novel method could be a practical and economically-sound tool to detect the host exposure to sand fly bites in CanL endemic areas.

No MeSH data available.


Related in: MedlinePlus

Dynamics of IgG antibody response against sand fly salivary proteins in dogs naturally exposed to P. perniciosus over two years in endemic foci.Canine sera were tested by ELISA for the antibodies recognizing SGH (open circle), rSP03B protein (open diamond) and combination of rSP03B+rSP01 proteins (open square). Data are presented as median values for each sampling month. Asterisk represents significant change in the median compared to previous sampling. ODx100 = optical density multiplied by 100.
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pntd.0003855.g002: Dynamics of IgG antibody response against sand fly salivary proteins in dogs naturally exposed to P. perniciosus over two years in endemic foci.Canine sera were tested by ELISA for the antibodies recognizing SGH (open circle), rSP03B protein (open diamond) and combination of rSP03B+rSP01 proteins (open square). Data are presented as median values for each sampling month. Asterisk represents significant change in the median compared to previous sampling. ODx100 = optical density multiplied by 100.

Mentions: The overall median values of ELISA ODx100 using SGH, rSP03B, and rSP03B+rSP01 antigens were 10 (range: 2–194), 24 (1–234) and 37 (11–189), respectively (p<0.05). However, the median increased significantly with time, following a similar pattern for all three antigens tested, most significantly for SGH and rSP03B (Fig 2). For example, the median ODx100 for SGH was 5 at the baseline in July of the first year, increased significantly to 11 through October, decreased thereafter to 8 in January, increased again up to a peak of 24 detected in July of the second year, and remained similar until September (Table 1). The sharp OD increase observed in summer of the second year for SGH and rSP03B was less pronounced for rSP03B+rSP01 (Table 1). Moreover, correlation of antibody response between SGH and rSP03B was stronger (r = 0.77) than between rSP03B+rSP01 and SGH (r = 0.65) (Fig 3). In addition, we detected high correlation between antibodies recognizing rSP03B and antibodies recognizing combination of rSP03B+rSP01 (r = 0.75) (Fig 3).


Canine Antibodies against Salivary Recombinant Proteins of Phlebotomus perniciosus: A Longitudinal Study in an Endemic Focus of Canine Leishmaniasis.

Kostalova T, Lestinova T, Sumova P, Vlkova M, Rohousova I, Berriatua E, Oliva G, Fiorentino E, Scalone A, Gramiccia M, Gradoni L, Volf P - PLoS Negl Trop Dis (2015)

Dynamics of IgG antibody response against sand fly salivary proteins in dogs naturally exposed to P. perniciosus over two years in endemic foci.Canine sera were tested by ELISA for the antibodies recognizing SGH (open circle), rSP03B protein (open diamond) and combination of rSP03B+rSP01 proteins (open square). Data are presented as median values for each sampling month. Asterisk represents significant change in the median compared to previous sampling. ODx100 = optical density multiplied by 100.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482481&req=5

pntd.0003855.g002: Dynamics of IgG antibody response against sand fly salivary proteins in dogs naturally exposed to P. perniciosus over two years in endemic foci.Canine sera were tested by ELISA for the antibodies recognizing SGH (open circle), rSP03B protein (open diamond) and combination of rSP03B+rSP01 proteins (open square). Data are presented as median values for each sampling month. Asterisk represents significant change in the median compared to previous sampling. ODx100 = optical density multiplied by 100.
Mentions: The overall median values of ELISA ODx100 using SGH, rSP03B, and rSP03B+rSP01 antigens were 10 (range: 2–194), 24 (1–234) and 37 (11–189), respectively (p<0.05). However, the median increased significantly with time, following a similar pattern for all three antigens tested, most significantly for SGH and rSP03B (Fig 2). For example, the median ODx100 for SGH was 5 at the baseline in July of the first year, increased significantly to 11 through October, decreased thereafter to 8 in January, increased again up to a peak of 24 detected in July of the second year, and remained similar until September (Table 1). The sharp OD increase observed in summer of the second year for SGH and rSP03B was less pronounced for rSP03B+rSP01 (Table 1). Moreover, correlation of antibody response between SGH and rSP03B was stronger (r = 0.77) than between rSP03B+rSP01 and SGH (r = 0.65) (Fig 3). In addition, we detected high correlation between antibodies recognizing rSP03B and antibodies recognizing combination of rSP03B+rSP01 (r = 0.75) (Fig 3).

Bottom Line: We found a significant association between active CanL infection and the amount of anti-P. perniciosus saliva antibodies.These results suggest that P. perniciosus rSP03B protein is a valid alternative to whole saliva and could be used in large-scale serological studies.This novel method could be a practical and economically-sound tool to detect the host exposure to sand fly bites in CanL endemic areas.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Faculty of Science, Charles University in Prague, Prague, Czech Republic.

ABSTRACT

Background: Phlebotomine sand flies are vectors of Leishmania parasites. During blood feeding, sand flies deposit into the host skin immunogenic salivary proteins which elicit specific antibody responses. These anti-saliva antibodies enable an estimate of the host exposure to sand flies and, in leishmaniasis endemic areas, also the risk for Leishmania infections. However, the use of whole salivary gland homogenates as antigen has several limitations, and therefore, recombinant salivary proteins have been tested to replace them in antibody detection assays. In this study, we have used for the first time sand fly salivary recombinant proteins in a longitudinal field study on dogs.

Methodology/principal findings: Sera from dogs naturally exposed to P. perniciosus bites over two consecutive transmission seasons in a site endemic for canine leishmaniasis (CanL) were tested at different time points by ELISA for the antibodies recognizing whole saliva, single salivary 43 kDa yellow-related recombinant protein (rSP03B), and a combination of two salivary recombinant proteins, 43 kDa yellow-related protein and 35.5 kDa apyrase (rSP01). Dogs were also tested for Leishmania infantum positivity by serology, culture, and PCR and the infection status was evaluated prospectively. We found a significant association between active CanL infection and the amount of anti-P. perniciosus saliva antibodies. Importantly, we detected a high correlation between IgG antibodies recognizing rSP03B protein and the whole salivary antigen. The kinetics of antibody response showed for both a whole saliva and rSP03B a similar pattern that was clearly related to the seasonal abundance of P. perniciosus.

Conclusions: These results suggest that P. perniciosus rSP03B protein is a valid alternative to whole saliva and could be used in large-scale serological studies. This novel method could be a practical and economically-sound tool to detect the host exposure to sand fly bites in CanL endemic areas.

No MeSH data available.


Related in: MedlinePlus