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Use of X-Chromosome Inactivation Pattern to Analyze the Clonality of 14 Female Cases of Kaposi Sarcoma.

Yuan D, XiuJuan W, Yan Z, JunQin L, Fang X, Shirong Y, Xiaojing K, Yanyan F, Weidong W, Dong L, Qingli L, DeZhi Z, XiongMing P - Med Sci Monit Basic Res (2015)

Bottom Line: Finally, we analyzed the clonality of KS.Heterozygosity of the PGK gene was observed in 5 (35.7%) cases, which all represent monoclonal origin.There was no significant difference according to country, stage, or HIV and HHV-8 (P>0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology and Venereology, The People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China (mainland).

ABSTRACT

Background: Kaposi sarcoma (KS) has features of both neoplastic growth and hyperplastic proliferation. It is the most common tumor seen in patients with HIV infection. Whether KS is a real tumor or a benign hyperplastic disease is not known.

Material and methods: Tissues from KS and cutaneous hemangioma lesion DNA were extracted, and then digested with methylation-sensitive restriction endonuclease HpaII. Human androgen receptor gene (HUMARA) was amplified with PCR method and the product was separated on 10% denaturing polyacrylamide gels and stained with ethylene dibromide (EB) to show the polymorphism of HUMARA. Phosphoglycerate kinase (PGK) was amplified and the product was digested by BStXI, agarose gel and EB stained to show the polymorphism of PGK. Finally, we analyzed the clonality of KS.

Results: In the 14 patients with KS, heterozygosity of the HUMARA gene was observed in 12 (85.7%) cases. Loss of heterozygosity of HUMARA gene on X-chromosome (without HpaII digestion there were 2 bands, after HpaII digestion there were just 1 of the bands), representing monoclonal origin, was present in 11 cases of Kaposi sarcoma. Heterozygosity of the PGK gene was observed in 5 (35.7%) cases, which all represent monoclonal origin. There was no significant difference according to country, stage, or HIV and HHV-8 (P>0.05).

Conclusions: The current findings suggest that Kaposi sarcoma is a clonal neoplasm, not a reactive proliferation.

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Related in: MedlinePlus

Clonality analysis in HUMARA (A) and PGK (B) gene of representative KS and cutaneous hemangioma. KS – Kaposi sarcoma; CA – cutaneous hemangioma; M – DNA marker; HpaII+ – with HpaII digestion; HpaII– – without HpaII digestion. KS indicated monoclonal samples. Before HpaII digestion there were 2 bands, and the intensity of the other band was lower. CA indicated polyclonal samples, with and without HpaII digestion; there all were 2 bands, but the latter band was lower intensity than the former band.
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f2-medscimonitbasicres-21-116: Clonality analysis in HUMARA (A) and PGK (B) gene of representative KS and cutaneous hemangioma. KS – Kaposi sarcoma; CA – cutaneous hemangioma; M – DNA marker; HpaII+ – with HpaII digestion; HpaII– – without HpaII digestion. KS indicated monoclonal samples. Before HpaII digestion there were 2 bands, and the intensity of the other band was lower. CA indicated polyclonal samples, with and without HpaII digestion; there all were 2 bands, but the latter band was lower intensity than the former band.

Mentions: In 2 of the 14 KS patients (cases 1 and 5) the DNA sample failed to amplify expected PCR products (HUMARA) visible on 2% agarose gel, while in 5 KS patients (1, 3, 4,9, and 12) the DNA sample succeed in amplifying (PGK). Eleven out of 12 KS tissues were analyzed by HUMARA gene and 5 KS tissues were analyzed by PGK gene. Without HpaII digestion there were 2 bands, and after HpaII digestion there was just 1 band (Figure 2). In 1 patient a polyclonal pattern was demonstrated in the KS tissue. These results suggest that KS is a clonal neoplasm rather than a reactive proliferation.


Use of X-Chromosome Inactivation Pattern to Analyze the Clonality of 14 Female Cases of Kaposi Sarcoma.

Yuan D, XiuJuan W, Yan Z, JunQin L, Fang X, Shirong Y, Xiaojing K, Yanyan F, Weidong W, Dong L, Qingli L, DeZhi Z, XiongMing P - Med Sci Monit Basic Res (2015)

Clonality analysis in HUMARA (A) and PGK (B) gene of representative KS and cutaneous hemangioma. KS – Kaposi sarcoma; CA – cutaneous hemangioma; M – DNA marker; HpaII+ – with HpaII digestion; HpaII– – without HpaII digestion. KS indicated monoclonal samples. Before HpaII digestion there were 2 bands, and the intensity of the other band was lower. CA indicated polyclonal samples, with and without HpaII digestion; there all were 2 bands, but the latter band was lower intensity than the former band.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4482332&req=5

f2-medscimonitbasicres-21-116: Clonality analysis in HUMARA (A) and PGK (B) gene of representative KS and cutaneous hemangioma. KS – Kaposi sarcoma; CA – cutaneous hemangioma; M – DNA marker; HpaII+ – with HpaII digestion; HpaII– – without HpaII digestion. KS indicated monoclonal samples. Before HpaII digestion there were 2 bands, and the intensity of the other band was lower. CA indicated polyclonal samples, with and without HpaII digestion; there all were 2 bands, but the latter band was lower intensity than the former band.
Mentions: In 2 of the 14 KS patients (cases 1 and 5) the DNA sample failed to amplify expected PCR products (HUMARA) visible on 2% agarose gel, while in 5 KS patients (1, 3, 4,9, and 12) the DNA sample succeed in amplifying (PGK). Eleven out of 12 KS tissues were analyzed by HUMARA gene and 5 KS tissues were analyzed by PGK gene. Without HpaII digestion there were 2 bands, and after HpaII digestion there was just 1 band (Figure 2). In 1 patient a polyclonal pattern was demonstrated in the KS tissue. These results suggest that KS is a clonal neoplasm rather than a reactive proliferation.

Bottom Line: Finally, we analyzed the clonality of KS.Heterozygosity of the PGK gene was observed in 5 (35.7%) cases, which all represent monoclonal origin.There was no significant difference according to country, stage, or HIV and HHV-8 (P>0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Dermatology and Venereology, The People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China (mainland).

ABSTRACT

Background: Kaposi sarcoma (KS) has features of both neoplastic growth and hyperplastic proliferation. It is the most common tumor seen in patients with HIV infection. Whether KS is a real tumor or a benign hyperplastic disease is not known.

Material and methods: Tissues from KS and cutaneous hemangioma lesion DNA were extracted, and then digested with methylation-sensitive restriction endonuclease HpaII. Human androgen receptor gene (HUMARA) was amplified with PCR method and the product was separated on 10% denaturing polyacrylamide gels and stained with ethylene dibromide (EB) to show the polymorphism of HUMARA. Phosphoglycerate kinase (PGK) was amplified and the product was digested by BStXI, agarose gel and EB stained to show the polymorphism of PGK. Finally, we analyzed the clonality of KS.

Results: In the 14 patients with KS, heterozygosity of the HUMARA gene was observed in 12 (85.7%) cases. Loss of heterozygosity of HUMARA gene on X-chromosome (without HpaII digestion there were 2 bands, after HpaII digestion there were just 1 of the bands), representing monoclonal origin, was present in 11 cases of Kaposi sarcoma. Heterozygosity of the PGK gene was observed in 5 (35.7%) cases, which all represent monoclonal origin. There was no significant difference according to country, stage, or HIV and HHV-8 (P>0.05).

Conclusions: The current findings suggest that Kaposi sarcoma is a clonal neoplasm, not a reactive proliferation.

Show MeSH
Related in: MedlinePlus