Limits...
Molecular Analysis by Gene Expression of Mitochondrial ATPase Subunits in Papillary Thyroid Cancer: Is ATP5E Transcript a Possible Early Tumor Marker?

Hurtado-López LM, Fernández-Ramírez F, Martínez-Peñafiel E, Carrillo Ruiz JD, Herrera González NE - Med. Sci. Monit. (2015)

Bottom Line: Thirty-five patients who had an operation for benign tumors were also included in the study. mRNA expression levels of alpha, beta, gamma, and epsilon subunits of F1 and "c12" of subunit Fo were determined by real-time RT-PCR (by duplicate), in order to determine if abnormal expression of these genes could partially explain the Warburg effect in papillary thyroid cancer (PTC).ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis OR=11.76 (95% confidence interval, 1.245-237.98; p=0.04).Relative down-expression of ATP5E transcript was highly associated with PTC diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Thyroid Clinic, Hospital General de Mexico, Instituto Politecnico Nacional, Mexico, Mexico.

ABSTRACT

Background: Cancer development involves an "injury" to the respiratory machinery (Warburg effect) due to decreased or impaired mitochondrial function. This circumstance results in a down regulation of some of the ATPase subunits of the malignant tissue. The objective of this work was to assess and compare the relative expression of mRNA of mitochondrial ATPase subunits between samples of thyroid cancer and benign nodules.

Material and methods: Samples from 31 patients who had an operation for PTC at the General Hospital of Mexico were snap-frozen and stored at -70°C. Thirty-five patients who had an operation for benign tumors were also included in the study. mRNA expression levels of alpha, beta, gamma, and epsilon subunits of F1 and "c12" of subunit Fo were determined by real-time RT-PCR (by duplicate), in order to determine if abnormal expression of these genes could partially explain the Warburg effect in papillary thyroid cancer (PTC).

Results: ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis OR=11.76 (95% confidence interval, 1.245-237.98; p=0.04).

Conclusions: Relative down-expression of ATP5E transcript was highly associated with PTC diagnosis. This transcript alteration may be used as a tumoral marker in papillary thyroid cancer.

No MeSH data available.


Related in: MedlinePlus

Clusters. At the level of diagnosis (X axis) all groups or clusters formed include both cancer (PTC) and goiter (CG).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4482184&req=5

f2-medscimonit-21-1745: Clusters. At the level of diagnosis (X axis) all groups or clusters formed include both cancer (PTC) and goiter (CG).

Mentions: According to several distance-based clustering algorithms (Figure 2), at the level of genes (Y axis), the behavior of ATP5G1 and ATP5G2 was similar since they are located in the same branch. The order of similarity was ATP5E, ATP5C1, and finally ATP5G2, all of which belong to 1 major cluster. The other cluster includes ATP5A1 and ATP5B, which segregate in a separate branch. This may represent biological coherence, as each major branch corresponds to F0 and F1 complexes, respectively.


Molecular Analysis by Gene Expression of Mitochondrial ATPase Subunits in Papillary Thyroid Cancer: Is ATP5E Transcript a Possible Early Tumor Marker?

Hurtado-López LM, Fernández-Ramírez F, Martínez-Peñafiel E, Carrillo Ruiz JD, Herrera González NE - Med. Sci. Monit. (2015)

Clusters. At the level of diagnosis (X axis) all groups or clusters formed include both cancer (PTC) and goiter (CG).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4482184&req=5

f2-medscimonit-21-1745: Clusters. At the level of diagnosis (X axis) all groups or clusters formed include both cancer (PTC) and goiter (CG).
Mentions: According to several distance-based clustering algorithms (Figure 2), at the level of genes (Y axis), the behavior of ATP5G1 and ATP5G2 was similar since they are located in the same branch. The order of similarity was ATP5E, ATP5C1, and finally ATP5G2, all of which belong to 1 major cluster. The other cluster includes ATP5A1 and ATP5B, which segregate in a separate branch. This may represent biological coherence, as each major branch corresponds to F0 and F1 complexes, respectively.

Bottom Line: Thirty-five patients who had an operation for benign tumors were also included in the study. mRNA expression levels of alpha, beta, gamma, and epsilon subunits of F1 and "c12" of subunit Fo were determined by real-time RT-PCR (by duplicate), in order to determine if abnormal expression of these genes could partially explain the Warburg effect in papillary thyroid cancer (PTC).ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis OR=11.76 (95% confidence interval, 1.245-237.98; p=0.04).Relative down-expression of ATP5E transcript was highly associated with PTC diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Thyroid Clinic, Hospital General de Mexico, Instituto Politecnico Nacional, Mexico, Mexico.

ABSTRACT

Background: Cancer development involves an "injury" to the respiratory machinery (Warburg effect) due to decreased or impaired mitochondrial function. This circumstance results in a down regulation of some of the ATPase subunits of the malignant tissue. The objective of this work was to assess and compare the relative expression of mRNA of mitochondrial ATPase subunits between samples of thyroid cancer and benign nodules.

Material and methods: Samples from 31 patients who had an operation for PTC at the General Hospital of Mexico were snap-frozen and stored at -70°C. Thirty-five patients who had an operation for benign tumors were also included in the study. mRNA expression levels of alpha, beta, gamma, and epsilon subunits of F1 and "c12" of subunit Fo were determined by real-time RT-PCR (by duplicate), in order to determine if abnormal expression of these genes could partially explain the Warburg effect in papillary thyroid cancer (PTC).

Results: ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis OR=11.76 (95% confidence interval, 1.245-237.98; p=0.04).

Conclusions: Relative down-expression of ATP5E transcript was highly associated with PTC diagnosis. This transcript alteration may be used as a tumoral marker in papillary thyroid cancer.

No MeSH data available.


Related in: MedlinePlus