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Molecular Analysis by Gene Expression of Mitochondrial ATPase Subunits in Papillary Thyroid Cancer: Is ATP5E Transcript a Possible Early Tumor Marker?

Hurtado-López LM, Fernández-Ramírez F, Martínez-Peñafiel E, Carrillo Ruiz JD, Herrera González NE - Med. Sci. Monit. (2015)

Bottom Line: Thirty-five patients who had an operation for benign tumors were also included in the study. mRNA expression levels of alpha, beta, gamma, and epsilon subunits of F1 and "c12" of subunit Fo were determined by real-time RT-PCR (by duplicate), in order to determine if abnormal expression of these genes could partially explain the Warburg effect in papillary thyroid cancer (PTC).ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis OR=11.76 (95% confidence interval, 1.245-237.98; p=0.04).Relative down-expression of ATP5E transcript was highly associated with PTC diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Thyroid Clinic, Hospital General de Mexico, Instituto Politecnico Nacional, Mexico, Mexico.

ABSTRACT

Background: Cancer development involves an "injury" to the respiratory machinery (Warburg effect) due to decreased or impaired mitochondrial function. This circumstance results in a down regulation of some of the ATPase subunits of the malignant tissue. The objective of this work was to assess and compare the relative expression of mRNA of mitochondrial ATPase subunits between samples of thyroid cancer and benign nodules.

Material and methods: Samples from 31 patients who had an operation for PTC at the General Hospital of Mexico were snap-frozen and stored at -70°C. Thirty-five patients who had an operation for benign tumors were also included in the study. mRNA expression levels of alpha, beta, gamma, and epsilon subunits of F1 and "c12" of subunit Fo were determined by real-time RT-PCR (by duplicate), in order to determine if abnormal expression of these genes could partially explain the Warburg effect in papillary thyroid cancer (PTC).

Results: ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis OR=11.76 (95% confidence interval, 1.245-237.98; p=0.04).

Conclusions: Relative down-expression of ATP5E transcript was highly associated with PTC diagnosis. This transcript alteration may be used as a tumoral marker in papillary thyroid cancer.

No MeSH data available.


Related in: MedlinePlus

Relative expression of ATP5E in colloid goiter and papillary thyroid cancer.
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f1-medscimonit-21-1745: Relative expression of ATP5E in colloid goiter and papillary thyroid cancer.

Mentions: Sixteen patients with PTC showed down-regulation of this transcript, of which 6 patients had equal to or less than Z=−1.96 and 15 showed up-regulation, of which none had equal to or more than Z=+1.96. The colloid goiter group had 9 patients with down-regulation of the transcript, of which none had equal to or less than Z=−1.96; 26 patients had up-regulation, of which none had equal to or more than Z=+1.96. The OR was 11.76 (95% confidence interval, 1.245–237.98) in comparing down-regulation (equal or less than Z=−1.96) and the 1-sided Fisher’s exact test score was p=0.045 (Figure 1).


Molecular Analysis by Gene Expression of Mitochondrial ATPase Subunits in Papillary Thyroid Cancer: Is ATP5E Transcript a Possible Early Tumor Marker?

Hurtado-López LM, Fernández-Ramírez F, Martínez-Peñafiel E, Carrillo Ruiz JD, Herrera González NE - Med. Sci. Monit. (2015)

Relative expression of ATP5E in colloid goiter and papillary thyroid cancer.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4482184&req=5

f1-medscimonit-21-1745: Relative expression of ATP5E in colloid goiter and papillary thyroid cancer.
Mentions: Sixteen patients with PTC showed down-regulation of this transcript, of which 6 patients had equal to or less than Z=−1.96 and 15 showed up-regulation, of which none had equal to or more than Z=+1.96. The colloid goiter group had 9 patients with down-regulation of the transcript, of which none had equal to or less than Z=−1.96; 26 patients had up-regulation, of which none had equal to or more than Z=+1.96. The OR was 11.76 (95% confidence interval, 1.245–237.98) in comparing down-regulation (equal or less than Z=−1.96) and the 1-sided Fisher’s exact test score was p=0.045 (Figure 1).

Bottom Line: Thirty-five patients who had an operation for benign tumors were also included in the study. mRNA expression levels of alpha, beta, gamma, and epsilon subunits of F1 and "c12" of subunit Fo were determined by real-time RT-PCR (by duplicate), in order to determine if abnormal expression of these genes could partially explain the Warburg effect in papillary thyroid cancer (PTC).ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis OR=11.76 (95% confidence interval, 1.245-237.98; p=0.04).Relative down-expression of ATP5E transcript was highly associated with PTC diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Thyroid Clinic, Hospital General de Mexico, Instituto Politecnico Nacional, Mexico, Mexico.

ABSTRACT

Background: Cancer development involves an "injury" to the respiratory machinery (Warburg effect) due to decreased or impaired mitochondrial function. This circumstance results in a down regulation of some of the ATPase subunits of the malignant tissue. The objective of this work was to assess and compare the relative expression of mRNA of mitochondrial ATPase subunits between samples of thyroid cancer and benign nodules.

Material and methods: Samples from 31 patients who had an operation for PTC at the General Hospital of Mexico were snap-frozen and stored at -70°C. Thirty-five patients who had an operation for benign tumors were also included in the study. mRNA expression levels of alpha, beta, gamma, and epsilon subunits of F1 and "c12" of subunit Fo were determined by real-time RT-PCR (by duplicate), in order to determine if abnormal expression of these genes could partially explain the Warburg effect in papillary thyroid cancer (PTC).

Results: ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis OR=11.76 (95% confidence interval, 1.245-237.98; p=0.04).

Conclusions: Relative down-expression of ATP5E transcript was highly associated with PTC diagnosis. This transcript alteration may be used as a tumoral marker in papillary thyroid cancer.

No MeSH data available.


Related in: MedlinePlus