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WIPPER: an accurate and efficient field phenotyping platform for large-scale applications.

Utsushi H, Abe A, Tamiru M, Ogasawara Y, Obara T, Sato E, Ochiai Y, Terauchi R, Takagi H - Breed. Sci. (2015)

Bottom Line: Additionally, it eliminates the risk of human error associated with phenotyping and inputting data.We applied WIPPER to 100 individuals of a rice recombinant inbred line (RIL) for measuring leaf width and relative chlorophyll content (SPAD value), and were able to record an accurate data in a significantly reduced time compared with the conventional method of data collection.We are currently using WIPPER for routine management of rice germplasm including recording and documenting information on phenotypic data, seeds, and DNA for their accelerated utilization in crop breeding.

View Article: PubMed Central - PubMed

Affiliation: Iwate Biotechnology Research Center , 22-174-4 Narita, Kitakami, Iwate 024-0003 , Japan.

ABSTRACT
More accurate, rapid, and easy phenotyping tools are required to match the recent advances in high-throughput genotyping for accelerating breeding and genetic analysis. The conventional data recording in field notebooks and then inputting data to computers for further analysis is inefficient, time-consuming, laborious, and prone to human error. Here, we report WIPPER (for Wireless Plant Phenotyper), a new phenotyping platform that combines field phenotyping and data recording with the aid of Bluetooth communication, thus saving time and labor not only for field data recoding but also for inputting data to computers. Additionally, it eliminates the risk of human error associated with phenotyping and inputting data. We applied WIPPER to 100 individuals of a rice recombinant inbred line (RIL) for measuring leaf width and relative chlorophyll content (SPAD value), and were able to record an accurate data in a significantly reduced time compared with the conventional method of data collection. We are currently using WIPPER for routine management of rice germplasm including recording and documenting information on phenotypic data, seeds, and DNA for their accelerated utilization in crop breeding.

No MeSH data available.


The application of WIPPER for an integrated germplasm and data management towards rapid genetic analysis. (A) Rice seeds stored in envelopes. Each envelope is labeled with a barcode containing all the necessary information about the line/accession. (B) DNA samples stored in barcoded tubes. (C) The concept of an integrated data management. For each line/accession, the same barcode or number is used to identify its phenotypic data, seeds, DNA sample, as well as genotyping data including whole genome sequences.
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f4-65_285: The application of WIPPER for an integrated germplasm and data management towards rapid genetic analysis. (A) Rice seeds stored in envelopes. Each envelope is labeled with a barcode containing all the necessary information about the line/accession. (B) DNA samples stored in barcoded tubes. (C) The concept of an integrated data management. For each line/accession, the same barcode or number is used to identify its phenotypic data, seeds, DNA sample, as well as genotyping data including whole genome sequences.

Mentions: Currently, we identify our rice germplasm including seeds and DNA samples of cultivated rice, wild relatives, and mutant populations using the same accession numbers given to each line for phenotyping. This involves packing seeds in barcoded envelopes or bags, as well as storing DNA samples in barcoded individual tubes (Fig. 4A, 4B). Our system ensures that researchers can easily access seeds or DNA samples of the individuals they want to further investigate based on phenotypes of interest. In future, we are planning to add genotyping data including whole genome sequence to the system (Fig. 4C). We believe that such systematic integration of relevant data will speed up genetic analysis and accelerate crop breeding, as well as improve cultivation techniques.


WIPPER: an accurate and efficient field phenotyping platform for large-scale applications.

Utsushi H, Abe A, Tamiru M, Ogasawara Y, Obara T, Sato E, Ochiai Y, Terauchi R, Takagi H - Breed. Sci. (2015)

The application of WIPPER for an integrated germplasm and data management towards rapid genetic analysis. (A) Rice seeds stored in envelopes. Each envelope is labeled with a barcode containing all the necessary information about the line/accession. (B) DNA samples stored in barcoded tubes. (C) The concept of an integrated data management. For each line/accession, the same barcode or number is used to identify its phenotypic data, seeds, DNA sample, as well as genotyping data including whole genome sequences.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482179&req=5

f4-65_285: The application of WIPPER for an integrated germplasm and data management towards rapid genetic analysis. (A) Rice seeds stored in envelopes. Each envelope is labeled with a barcode containing all the necessary information about the line/accession. (B) DNA samples stored in barcoded tubes. (C) The concept of an integrated data management. For each line/accession, the same barcode or number is used to identify its phenotypic data, seeds, DNA sample, as well as genotyping data including whole genome sequences.
Mentions: Currently, we identify our rice germplasm including seeds and DNA samples of cultivated rice, wild relatives, and mutant populations using the same accession numbers given to each line for phenotyping. This involves packing seeds in barcoded envelopes or bags, as well as storing DNA samples in barcoded individual tubes (Fig. 4A, 4B). Our system ensures that researchers can easily access seeds or DNA samples of the individuals they want to further investigate based on phenotypes of interest. In future, we are planning to add genotyping data including whole genome sequence to the system (Fig. 4C). We believe that such systematic integration of relevant data will speed up genetic analysis and accelerate crop breeding, as well as improve cultivation techniques.

Bottom Line: Additionally, it eliminates the risk of human error associated with phenotyping and inputting data.We applied WIPPER to 100 individuals of a rice recombinant inbred line (RIL) for measuring leaf width and relative chlorophyll content (SPAD value), and were able to record an accurate data in a significantly reduced time compared with the conventional method of data collection.We are currently using WIPPER for routine management of rice germplasm including recording and documenting information on phenotypic data, seeds, and DNA for their accelerated utilization in crop breeding.

View Article: PubMed Central - PubMed

Affiliation: Iwate Biotechnology Research Center , 22-174-4 Narita, Kitakami, Iwate 024-0003 , Japan.

ABSTRACT
More accurate, rapid, and easy phenotyping tools are required to match the recent advances in high-throughput genotyping for accelerating breeding and genetic analysis. The conventional data recording in field notebooks and then inputting data to computers for further analysis is inefficient, time-consuming, laborious, and prone to human error. Here, we report WIPPER (for Wireless Plant Phenotyper), a new phenotyping platform that combines field phenotyping and data recording with the aid of Bluetooth communication, thus saving time and labor not only for field data recoding but also for inputting data to computers. Additionally, it eliminates the risk of human error associated with phenotyping and inputting data. We applied WIPPER to 100 individuals of a rice recombinant inbred line (RIL) for measuring leaf width and relative chlorophyll content (SPAD value), and were able to record an accurate data in a significantly reduced time compared with the conventional method of data collection. We are currently using WIPPER for routine management of rice germplasm including recording and documenting information on phenotypic data, seeds, and DNA for their accelerated utilization in crop breeding.

No MeSH data available.