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WIPPER: an accurate and efficient field phenotyping platform for large-scale applications.

Utsushi H, Abe A, Tamiru M, Ogasawara Y, Obara T, Sato E, Ochiai Y, Terauchi R, Takagi H - Breed. Sci. (2015)

Bottom Line: Additionally, it eliminates the risk of human error associated with phenotyping and inputting data.We applied WIPPER to 100 individuals of a rice recombinant inbred line (RIL) for measuring leaf width and relative chlorophyll content (SPAD value), and were able to record an accurate data in a significantly reduced time compared with the conventional method of data collection.We are currently using WIPPER for routine management of rice germplasm including recording and documenting information on phenotypic data, seeds, and DNA for their accelerated utilization in crop breeding.

View Article: PubMed Central - PubMed

Affiliation: Iwate Biotechnology Research Center , 22-174-4 Narita, Kitakami, Iwate 024-0003 , Japan.

ABSTRACT
More accurate, rapid, and easy phenotyping tools are required to match the recent advances in high-throughput genotyping for accelerating breeding and genetic analysis. The conventional data recording in field notebooks and then inputting data to computers for further analysis is inefficient, time-consuming, laborious, and prone to human error. Here, we report WIPPER (for Wireless Plant Phenotyper), a new phenotyping platform that combines field phenotyping and data recording with the aid of Bluetooth communication, thus saving time and labor not only for field data recoding but also for inputting data to computers. Additionally, it eliminates the risk of human error associated with phenotyping and inputting data. We applied WIPPER to 100 individuals of a rice recombinant inbred line (RIL) for measuring leaf width and relative chlorophyll content (SPAD value), and were able to record an accurate data in a significantly reduced time compared with the conventional method of data collection. We are currently using WIPPER for routine management of rice germplasm including recording and documenting information on phenotypic data, seeds, and DNA for their accelerated utilization in crop breeding.

No MeSH data available.


Measuring SPAD value in rice recombinant inbred lines by WIPPER. (A) Identifying each line/accession by scanning its corresponding barcode using a smart phone. (B) An SPAD502 meter with Bluetooth connector is used for measuring SPAD values. Blue circle indicates Bluetooth connector. (C) Scheme of the cloud storage server applied in this study. (D) Comparison of the time taken (in minutes) for measuring SPAD values of 100 individual plants (5 individuals from each of 20 lines). Asterisks indicate significant difference (Student’s t-test, * P < 0.05, ** P < 0.01; normality of variables and equality of variances were validated by Kolmogorov-Smirnov test and F-test, respectively).
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f3-65_285: Measuring SPAD value in rice recombinant inbred lines by WIPPER. (A) Identifying each line/accession by scanning its corresponding barcode using a smart phone. (B) An SPAD502 meter with Bluetooth connector is used for measuring SPAD values. Blue circle indicates Bluetooth connector. (C) Scheme of the cloud storage server applied in this study. (D) Comparison of the time taken (in minutes) for measuring SPAD values of 100 individual plants (5 individuals from each of 20 lines). Asterisks indicate significant difference (Student’s t-test, * P < 0.05, ** P < 0.01; normality of variables and equality of variances were validated by Kolmogorov-Smirnov test and F-test, respectively).

Mentions: Because the amount and time of availability of essential plant nutrients determine crop growth and yield, SPAD value is usually used as an important indicator of the relative chlorophyll content of leaves for timing fertilizer application in crop fields including rice (Fred et al. 1991). To this end, SPAD value has to be continuously monitored during the growing period. For effective monitoring of SPAD values, we also applied the WIPPER system as described in Fig. 3. In this investigation, mobile devices such as smart phone and tablet PC were used as substitutes for Barcode reader (Fig. 3A). Accordingly, the mobile devices were first used to scan the bar code labels similar to the way it was done for leaf width measurement. Next, SPAD values measured with the modified SPAD-502 meter equipped with Bluetooth connector were automatically transferred into the mobile device by Bluetooth wireless communication (Fig. 3B). Finally, the SPAD values recorded in mobile device were transferred automatically into cloud storage server, which was designated to each user in Wi-Fi environment (Fig. 3C).


WIPPER: an accurate and efficient field phenotyping platform for large-scale applications.

Utsushi H, Abe A, Tamiru M, Ogasawara Y, Obara T, Sato E, Ochiai Y, Terauchi R, Takagi H - Breed. Sci. (2015)

Measuring SPAD value in rice recombinant inbred lines by WIPPER. (A) Identifying each line/accession by scanning its corresponding barcode using a smart phone. (B) An SPAD502 meter with Bluetooth connector is used for measuring SPAD values. Blue circle indicates Bluetooth connector. (C) Scheme of the cloud storage server applied in this study. (D) Comparison of the time taken (in minutes) for measuring SPAD values of 100 individual plants (5 individuals from each of 20 lines). Asterisks indicate significant difference (Student’s t-test, * P < 0.05, ** P < 0.01; normality of variables and equality of variances were validated by Kolmogorov-Smirnov test and F-test, respectively).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482179&req=5

f3-65_285: Measuring SPAD value in rice recombinant inbred lines by WIPPER. (A) Identifying each line/accession by scanning its corresponding barcode using a smart phone. (B) An SPAD502 meter with Bluetooth connector is used for measuring SPAD values. Blue circle indicates Bluetooth connector. (C) Scheme of the cloud storage server applied in this study. (D) Comparison of the time taken (in minutes) for measuring SPAD values of 100 individual plants (5 individuals from each of 20 lines). Asterisks indicate significant difference (Student’s t-test, * P < 0.05, ** P < 0.01; normality of variables and equality of variances were validated by Kolmogorov-Smirnov test and F-test, respectively).
Mentions: Because the amount and time of availability of essential plant nutrients determine crop growth and yield, SPAD value is usually used as an important indicator of the relative chlorophyll content of leaves for timing fertilizer application in crop fields including rice (Fred et al. 1991). To this end, SPAD value has to be continuously monitored during the growing period. For effective monitoring of SPAD values, we also applied the WIPPER system as described in Fig. 3. In this investigation, mobile devices such as smart phone and tablet PC were used as substitutes for Barcode reader (Fig. 3A). Accordingly, the mobile devices were first used to scan the bar code labels similar to the way it was done for leaf width measurement. Next, SPAD values measured with the modified SPAD-502 meter equipped with Bluetooth connector were automatically transferred into the mobile device by Bluetooth wireless communication (Fig. 3B). Finally, the SPAD values recorded in mobile device were transferred automatically into cloud storage server, which was designated to each user in Wi-Fi environment (Fig. 3C).

Bottom Line: Additionally, it eliminates the risk of human error associated with phenotyping and inputting data.We applied WIPPER to 100 individuals of a rice recombinant inbred line (RIL) for measuring leaf width and relative chlorophyll content (SPAD value), and were able to record an accurate data in a significantly reduced time compared with the conventional method of data collection.We are currently using WIPPER for routine management of rice germplasm including recording and documenting information on phenotypic data, seeds, and DNA for their accelerated utilization in crop breeding.

View Article: PubMed Central - PubMed

Affiliation: Iwate Biotechnology Research Center , 22-174-4 Narita, Kitakami, Iwate 024-0003 , Japan.

ABSTRACT
More accurate, rapid, and easy phenotyping tools are required to match the recent advances in high-throughput genotyping for accelerating breeding and genetic analysis. The conventional data recording in field notebooks and then inputting data to computers for further analysis is inefficient, time-consuming, laborious, and prone to human error. Here, we report WIPPER (for Wireless Plant Phenotyper), a new phenotyping platform that combines field phenotyping and data recording with the aid of Bluetooth communication, thus saving time and labor not only for field data recoding but also for inputting data to computers. Additionally, it eliminates the risk of human error associated with phenotyping and inputting data. We applied WIPPER to 100 individuals of a rice recombinant inbred line (RIL) for measuring leaf width and relative chlorophyll content (SPAD value), and were able to record an accurate data in a significantly reduced time compared with the conventional method of data collection. We are currently using WIPPER for routine management of rice germplasm including recording and documenting information on phenotypic data, seeds, and DNA for their accelerated utilization in crop breeding.

No MeSH data available.