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Characterization of FAE1 in the zero erucic acid germplasm of Brassica rapa L.

Yan G, Li D, Cai M, Gao G, Chen B, Xu K, Li J, Li F, Wang N, Qiao J, Li H, Zhang T, Wu X - Breed. Sci. (2015)

Bottom Line: Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression.This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation.The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture , Wuhan 430062 , P. R. China.

ABSTRACT
The modification of erucic acid content in seeds is one of the major goals for quality breeding in oil-yielding Brassica species. However, few low erucic acid (LEA) resources are available, and novel LEA genetic resources are being sought. Fatty acid elongase 1 (FAE1) is the key gene that controls erucic acid synthesis. However, the mechanism for erucic acid synthesis in B. rapa lacks systematic study. Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression. Moreover, the FAE1 promoter sequences of LEA and high erucic acid materials shared 95% similarity. Twenty-eight bases deletions (containing a 24-base AT-rich region) were identified approximately 1300 bp upstream from the FAE1 start codon in the LEA accessions. The genotype with the deletions co-segregated with the LEA trait in the segregating population. This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation. The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

No MeSH data available.


The sequences alignment results of the HEA and LEA FAE1 promoters. N: HEA B. rapa Nanhualinggongdacaizi (accession number: KF999632). S: LEA B. rapa Sanjiecaizi (accession number: KF999615). H: LEA B. rapa HJa 96368 (accession number: KF999623). Q: LEA B. rapa Qingyou 11 (accession number: KP718763). ▭ The A/T-rich sequence deleted from LEA FAE1 promoters. The promoter regions do not contain CpG islands. The sequence data presented here have been submitted to GenBank. → The positions of pM120F and pM468R.
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f4-65_257: The sequences alignment results of the HEA and LEA FAE1 promoters. N: HEA B. rapa Nanhualinggongdacaizi (accession number: KF999632). S: LEA B. rapa Sanjiecaizi (accession number: KF999615). H: LEA B. rapa HJa 96368 (accession number: KF999623). Q: LEA B. rapa Qingyou 11 (accession number: KP718763). ▭ The A/T-rich sequence deleted from LEA FAE1 promoters. The promoter regions do not contain CpG islands. The sequence data presented here have been submitted to GenBank. → The positions of pM120F and pM468R.

Mentions: The promoter regions of the FAE1 gene from the LEA B. rapa Sanjiecaizi and Qingyou 11 and HJa 96368, and the HEA Nanhualinggongdacaizi were isolated based on the Chiifu-401-42 sequence (Wang et al. 2011) (Fig. 1). The alignment results showed that the three LEA promoter sequences shared 95% similarity with the HEA sequences; however, the sequence of the landrace LEA Sanjiecaizi was 100% similar to the LEA cultivar Qingyou 11 and the foreign cultivar HJa 96368. There were 56 SNPs and 37 INDELs between the HEA and LEA promoter sequences. Among the INDELs, a total of 28 bases were deleted at a position approximately 1300 bp from the translation initiation site (ATG) of the LEA allele promoter (Fig. 4). The deletions caused the LEA sequence to lose a 24-base AT-rich region. The variations were mainly located in the region shown in Fig. 4.


Characterization of FAE1 in the zero erucic acid germplasm of Brassica rapa L.

Yan G, Li D, Cai M, Gao G, Chen B, Xu K, Li J, Li F, Wang N, Qiao J, Li H, Zhang T, Wu X - Breed. Sci. (2015)

The sequences alignment results of the HEA and LEA FAE1 promoters. N: HEA B. rapa Nanhualinggongdacaizi (accession number: KF999632). S: LEA B. rapa Sanjiecaizi (accession number: KF999615). H: LEA B. rapa HJa 96368 (accession number: KF999623). Q: LEA B. rapa Qingyou 11 (accession number: KP718763). ▭ The A/T-rich sequence deleted from LEA FAE1 promoters. The promoter regions do not contain CpG islands. The sequence data presented here have been submitted to GenBank. → The positions of pM120F and pM468R.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482176&req=5

f4-65_257: The sequences alignment results of the HEA and LEA FAE1 promoters. N: HEA B. rapa Nanhualinggongdacaizi (accession number: KF999632). S: LEA B. rapa Sanjiecaizi (accession number: KF999615). H: LEA B. rapa HJa 96368 (accession number: KF999623). Q: LEA B. rapa Qingyou 11 (accession number: KP718763). ▭ The A/T-rich sequence deleted from LEA FAE1 promoters. The promoter regions do not contain CpG islands. The sequence data presented here have been submitted to GenBank. → The positions of pM120F and pM468R.
Mentions: The promoter regions of the FAE1 gene from the LEA B. rapa Sanjiecaizi and Qingyou 11 and HJa 96368, and the HEA Nanhualinggongdacaizi were isolated based on the Chiifu-401-42 sequence (Wang et al. 2011) (Fig. 1). The alignment results showed that the three LEA promoter sequences shared 95% similarity with the HEA sequences; however, the sequence of the landrace LEA Sanjiecaizi was 100% similar to the LEA cultivar Qingyou 11 and the foreign cultivar HJa 96368. There were 56 SNPs and 37 INDELs between the HEA and LEA promoter sequences. Among the INDELs, a total of 28 bases were deleted at a position approximately 1300 bp from the translation initiation site (ATG) of the LEA allele promoter (Fig. 4). The deletions caused the LEA sequence to lose a 24-base AT-rich region. The variations were mainly located in the region shown in Fig. 4.

Bottom Line: Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression.This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation.The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture , Wuhan 430062 , P. R. China.

ABSTRACT
The modification of erucic acid content in seeds is one of the major goals for quality breeding in oil-yielding Brassica species. However, few low erucic acid (LEA) resources are available, and novel LEA genetic resources are being sought. Fatty acid elongase 1 (FAE1) is the key gene that controls erucic acid synthesis. However, the mechanism for erucic acid synthesis in B. rapa lacks systematic study. Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression. Moreover, the FAE1 promoter sequences of LEA and high erucic acid materials shared 95% similarity. Twenty-eight bases deletions (containing a 24-base AT-rich region) were identified approximately 1300 bp upstream from the FAE1 start codon in the LEA accessions. The genotype with the deletions co-segregated with the LEA trait in the segregating population. This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation. The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

No MeSH data available.