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Characterization of FAE1 in the zero erucic acid germplasm of Brassica rapa L.

Yan G, Li D, Cai M, Gao G, Chen B, Xu K, Li J, Li F, Wang N, Qiao J, Li H, Zhang T, Wu X - Breed. Sci. (2015)

Bottom Line: Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression.This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation.The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture , Wuhan 430062 , P. R. China.

ABSTRACT
The modification of erucic acid content in seeds is one of the major goals for quality breeding in oil-yielding Brassica species. However, few low erucic acid (LEA) resources are available, and novel LEA genetic resources are being sought. Fatty acid elongase 1 (FAE1) is the key gene that controls erucic acid synthesis. However, the mechanism for erucic acid synthesis in B. rapa lacks systematic study. Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression. Moreover, the FAE1 promoter sequences of LEA and high erucic acid materials shared 95% similarity. Twenty-eight bases deletions (containing a 24-base AT-rich region) were identified approximately 1300 bp upstream from the FAE1 start codon in the LEA accessions. The genotype with the deletions co-segregated with the LEA trait in the segregating population. This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation. The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

No MeSH data available.


GC analysis showing fatty acid profiles of different individuals of Sanjiecaizi. a: Sanjiecaizi-1 (erucic acid content: 0); b: Sanjiecaizi-28 (erucic acid content: 13.84%); c: Sanjiecaizi-49 (erucic acid content: 33.22%).
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f2-65_257: GC analysis showing fatty acid profiles of different individuals of Sanjiecaizi. a: Sanjiecaizi-1 (erucic acid content: 0); b: Sanjiecaizi-28 (erucic acid content: 13.84%); c: Sanjiecaizi-49 (erucic acid content: 33.22%).

Mentions: Half-seed GC analysis of the 17 landraces with 10–20% erucic acid showed that their erucic acid contents varied from 0% to 36.6% (data not shown). The Sanjiecaizi individuals had the largest variation of erucic acid content (varying from 0 to 34%) among the 17 landraces, and the erucic acid contents of the other 16 landraces varied from 11.0% to 36.6%. Among the fifty seeds of the Sanjiecaizi analyzed, in 26 seeds (52%), the erucic acid contents were zero; in three seeds (6%), between 10% and 20%; in thirteen seeds (26%), between 20% and 30%; and in eight seeds (16%), between 30% and 40% (Fig. 2, Supplemental Table 2). The Sanjiecaizi individuals with zero erucic acid were planted and self-pollinated, and we ultimately obtained stable zero erucic acid B. rapa lines through several self-pollinations.


Characterization of FAE1 in the zero erucic acid germplasm of Brassica rapa L.

Yan G, Li D, Cai M, Gao G, Chen B, Xu K, Li J, Li F, Wang N, Qiao J, Li H, Zhang T, Wu X - Breed. Sci. (2015)

GC analysis showing fatty acid profiles of different individuals of Sanjiecaizi. a: Sanjiecaizi-1 (erucic acid content: 0); b: Sanjiecaizi-28 (erucic acid content: 13.84%); c: Sanjiecaizi-49 (erucic acid content: 33.22%).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482176&req=5

f2-65_257: GC analysis showing fatty acid profiles of different individuals of Sanjiecaizi. a: Sanjiecaizi-1 (erucic acid content: 0); b: Sanjiecaizi-28 (erucic acid content: 13.84%); c: Sanjiecaizi-49 (erucic acid content: 33.22%).
Mentions: Half-seed GC analysis of the 17 landraces with 10–20% erucic acid showed that their erucic acid contents varied from 0% to 36.6% (data not shown). The Sanjiecaizi individuals had the largest variation of erucic acid content (varying from 0 to 34%) among the 17 landraces, and the erucic acid contents of the other 16 landraces varied from 11.0% to 36.6%. Among the fifty seeds of the Sanjiecaizi analyzed, in 26 seeds (52%), the erucic acid contents were zero; in three seeds (6%), between 10% and 20%; in thirteen seeds (26%), between 20% and 30%; and in eight seeds (16%), between 30% and 40% (Fig. 2, Supplemental Table 2). The Sanjiecaizi individuals with zero erucic acid were planted and self-pollinated, and we ultimately obtained stable zero erucic acid B. rapa lines through several self-pollinations.

Bottom Line: Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression.This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation.The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture , Wuhan 430062 , P. R. China.

ABSTRACT
The modification of erucic acid content in seeds is one of the major goals for quality breeding in oil-yielding Brassica species. However, few low erucic acid (LEA) resources are available, and novel LEA genetic resources are being sought. Fatty acid elongase 1 (FAE1) is the key gene that controls erucic acid synthesis. However, the mechanism for erucic acid synthesis in B. rapa lacks systematic study. Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression. Moreover, the FAE1 promoter sequences of LEA and high erucic acid materials shared 95% similarity. Twenty-eight bases deletions (containing a 24-base AT-rich region) were identified approximately 1300 bp upstream from the FAE1 start codon in the LEA accessions. The genotype with the deletions co-segregated with the LEA trait in the segregating population. This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation. The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

No MeSH data available.