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Characterization of FAE1 in the zero erucic acid germplasm of Brassica rapa L.

Yan G, Li D, Cai M, Gao G, Chen B, Xu K, Li J, Li F, Wang N, Qiao J, Li H, Zhang T, Wu X - Breed. Sci. (2015)

Bottom Line: Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression.This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation.The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture , Wuhan 430062 , P. R. China.

ABSTRACT
The modification of erucic acid content in seeds is one of the major goals for quality breeding in oil-yielding Brassica species. However, few low erucic acid (LEA) resources are available, and novel LEA genetic resources are being sought. Fatty acid elongase 1 (FAE1) is the key gene that controls erucic acid synthesis. However, the mechanism for erucic acid synthesis in B. rapa lacks systematic study. Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression. Moreover, the FAE1 promoter sequences of LEA and high erucic acid materials shared 95% similarity. Twenty-eight bases deletions (containing a 24-base AT-rich region) were identified approximately 1300 bp upstream from the FAE1 start codon in the LEA accessions. The genotype with the deletions co-segregated with the LEA trait in the segregating population. This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation. The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

No MeSH data available.


Schematic representation of the position of FAE1 and its promoter in the B. rapa chromosome.
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f1-65_257: Schematic representation of the position of FAE1 and its promoter in the B. rapa chromosome.

Mentions: Based on the FAE1 sequences from BRAD Bra034635.1 (Wang et al. 2011, http://www.brassicadb.org), the forward primer (FAEF1: 5′-ATTCTCCGACACACACACTG-3′) and the reverse primer (FAER1: 5′-AGAGAAACATCGTAGCCATCA-3′) were designed to isolate the CDS of FAE1. To obtain the full length of the upstream sequence of FAE1, one pair of specific primers (PFAE40F: 5′-TGCATCCATAGATATCCTGT-3′; PPR: 5′-AACGGAAAGAAGCAAAGGT-3′) was designed within the 3′ and 5′ sequences of Bra034634 and FAE1. The primers of pM120F (5′-TCGGTAAAAGAAAAATCA-3′) and pM468R (5′-CTCATCTAAACTATATTAAGTG-3′) were designed based on the deletions of the LEA promoter for genotyping the segregating population (Fig. 1).


Characterization of FAE1 in the zero erucic acid germplasm of Brassica rapa L.

Yan G, Li D, Cai M, Gao G, Chen B, Xu K, Li J, Li F, Wang N, Qiao J, Li H, Zhang T, Wu X - Breed. Sci. (2015)

Schematic representation of the position of FAE1 and its promoter in the B. rapa chromosome.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482176&req=5

f1-65_257: Schematic representation of the position of FAE1 and its promoter in the B. rapa chromosome.
Mentions: Based on the FAE1 sequences from BRAD Bra034635.1 (Wang et al. 2011, http://www.brassicadb.org), the forward primer (FAEF1: 5′-ATTCTCCGACACACACACTG-3′) and the reverse primer (FAER1: 5′-AGAGAAACATCGTAGCCATCA-3′) were designed to isolate the CDS of FAE1. To obtain the full length of the upstream sequence of FAE1, one pair of specific primers (PFAE40F: 5′-TGCATCCATAGATATCCTGT-3′; PPR: 5′-AACGGAAAGAAGCAAAGGT-3′) was designed within the 3′ and 5′ sequences of Bra034634 and FAE1. The primers of pM120F (5′-TCGGTAAAAGAAAAATCA-3′) and pM468R (5′-CTCATCTAAACTATATTAAGTG-3′) were designed based on the deletions of the LEA promoter for genotyping the segregating population (Fig. 1).

Bottom Line: Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression.This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation.The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

View Article: PubMed Central - PubMed

Affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture , Wuhan 430062 , P. R. China.

ABSTRACT
The modification of erucic acid content in seeds is one of the major goals for quality breeding in oil-yielding Brassica species. However, few low erucic acid (LEA) resources are available, and novel LEA genetic resources are being sought. Fatty acid elongase 1 (FAE1) is the key gene that controls erucic acid synthesis. However, the mechanism for erucic acid synthesis in B. rapa lacks systematic study. Here, we isolated zero erucic acid lines from 1981 Chinese landraces of B. rapa and found that the formation of LEA is not attributable to variations in FAE1 coding sequences, as reported for B. napus, but may be attributable to the decrease in FAE1 expression. Moreover, the FAE1 promoter sequences of LEA and high erucic acid materials shared 95% similarity. Twenty-eight bases deletions (containing a 24-base AT-rich region) were identified approximately 1300 bp upstream from the FAE1 start codon in the LEA accessions. The genotype with the deletions co-segregated with the LEA trait in the segregating population. This study isolated an LEA B. rapa resource that can be exploited in Brassica cultivation. The promoter variations might modify the expression level of FAE1, and the results shed light on novel regulation mechanisms for erucic acid synthesis.

No MeSH data available.