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Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants.

Withanage SP, Hossain MA, Kumar M S, Roslan HA, Abdullah MP, Napis SB, Shukor NA - Breed. Sci. (2015)

Bottom Line: Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene.The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition.Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia , 43400 UPM, Serdang, Selangor , Malaysia.

ABSTRACT
Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in bast and 1.28 mm in whole plant) is below the critical length (4 mm) for biocomposite production. Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene. AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition. Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.

No MeSH data available.


Related in: MedlinePlus

Fiber derivative values flexibility co-efficient (a), slenderness ratio (b) and runkel ratio (c) of bast and core fibbers of transgenic G4 and V36 kenaf plants and their respective UT.
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f6-65_177: Fiber derivative values flexibility co-efficient (a), slenderness ratio (b) and runkel ratio (c) of bast and core fibbers of transgenic G4 and V36 kenaf plants and their respective UT.

Mentions: Both the bast and core flexibility ratios of all the transgenic line of the varieties G4 (except line G4-4) and V36 (except V36-13) were significantly (p ≤ 0.05) lower than those of the UT (Fig. 6). In bast slenderness ratio, lines G4-1 and V36-1 showed higher values (162 and 165, respectively) compared to their respective UT plants, with only V36-1 was significantly different. In core, all the lines in G4 showed slightly higher values (ranging from 35 to 38) compared to UT (33) but they did not vary meaningfully. In the variety V36, the slenderness ratio was slightly lower in the transgenic lines (37 to 40) compared to its UT (45) although with no significant difference (p < 0.05) (Fig. 6). The value of the runkel ratio for bast and core in both the varieties was remarkably higher in all the transgenic lines compared to their UT plants (Fig. 6). The highest runkel ratio (4) was observed in line G4-7 and V36-3 in bast and in V36-13 (3.9) for core compared to their UT plants (0.7 and 0.4 for G4 and V36, respectively).


Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants.

Withanage SP, Hossain MA, Kumar M S, Roslan HA, Abdullah MP, Napis SB, Shukor NA - Breed. Sci. (2015)

Fiber derivative values flexibility co-efficient (a), slenderness ratio (b) and runkel ratio (c) of bast and core fibbers of transgenic G4 and V36 kenaf plants and their respective UT.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482167&req=5

f6-65_177: Fiber derivative values flexibility co-efficient (a), slenderness ratio (b) and runkel ratio (c) of bast and core fibbers of transgenic G4 and V36 kenaf plants and their respective UT.
Mentions: Both the bast and core flexibility ratios of all the transgenic line of the varieties G4 (except line G4-4) and V36 (except V36-13) were significantly (p ≤ 0.05) lower than those of the UT (Fig. 6). In bast slenderness ratio, lines G4-1 and V36-1 showed higher values (162 and 165, respectively) compared to their respective UT plants, with only V36-1 was significantly different. In core, all the lines in G4 showed slightly higher values (ranging from 35 to 38) compared to UT (33) but they did not vary meaningfully. In the variety V36, the slenderness ratio was slightly lower in the transgenic lines (37 to 40) compared to its UT (45) although with no significant difference (p < 0.05) (Fig. 6). The value of the runkel ratio for bast and core in both the varieties was remarkably higher in all the transgenic lines compared to their UT plants (Fig. 6). The highest runkel ratio (4) was observed in line G4-7 and V36-3 in bast and in V36-13 (3.9) for core compared to their UT plants (0.7 and 0.4 for G4 and V36, respectively).

Bottom Line: Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene.The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition.Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia , 43400 UPM, Serdang, Selangor , Malaysia.

ABSTRACT
Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in bast and 1.28 mm in whole plant) is below the critical length (4 mm) for biocomposite production. Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene. AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition. Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.

No MeSH data available.


Related in: MedlinePlus