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Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants.

Withanage SP, Hossain MA, Kumar M S, Roslan HA, Abdullah MP, Napis SB, Shukor NA - Breed. Sci. (2015)

Bottom Line: Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene.The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition.Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia , 43400 UPM, Serdang, Selangor , Malaysia.

ABSTRACT
Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in bast and 1.28 mm in whole plant) is below the critical length (4 mm) for biocomposite production. Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene. AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition. Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.

No MeSH data available.


Related in: MedlinePlus

Effects of gibberellins on fiber length and diameter in transgenic kenaf and their respective UT plants. Length (a) and diameter (b) of bast and core fibers of transgenic G4 and V36 lines and their respective UT. Microscopic images of bast fiber length of the transgenic lines compared to their respective UT(c).
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f4-65_177: Effects of gibberellins on fiber length and diameter in transgenic kenaf and their respective UT plants. Length (a) and diameter (b) of bast and core fibers of transgenic G4 and V36 lines and their respective UT. Microscopic images of bast fiber length of the transgenic lines compared to their respective UT(c).

Mentions: The fiber length of the transgenic kenaf plant was significantly varied from the UT plant of both the varieties for bast, but not for the core. The mean bast fiber length of line G4-1 and G4-2 showed the highest values 2.80 mm compared to their UT plants (2.6 mm). The mean bast fiber length of V36-1 was 3.22 mm compared to 1.5 mm for their UT plants (Fig. 4a, c). The average length of the core fiber for all the transgenic lines (above 1 mm) was greater than that of the UT plants (around 0.8 mm) (Fig. 4a). Nonetheless, no significant (p ≤ 0.05) difference was observed in the fiber diameter of the transgenic lines from the UT line for bast and core (except the V36-1 in core) in both varieties although they were slightly higher to UT (Fig. 4b). In contrast to fiber diameter, the lumen diameter of all the transgenic lines in both G4 and V36 was comparatively lower for core than the UT plants but there was no significant difference in bast compared to UT (Fig. 5). The core lumen diameter of the transgenic lines of G4-1 ranged from 13.7 μm to 15.7 μm compared to 21.4 μm in the UT. All the transgenic lines showed higher cell wall thickness for both bast and core compared to their respective controls (Fig. 5). Accordingly, lines G4-1, G4-2, G4-4, G4-5, V36-1 and V36-13 had core cell wall thicknesses that were highly significant at p < 0.05. In the bast cell wall, lines G4-2, G4-5 and G4-7 showed significantly higher thicknesses whereas these were marginally different in other lines.


Overexpression of Arabidopsis thaliana gibberellic acid 20 oxidase (AtGA20ox) gene enhance the vegetative growth and fiber quality in kenaf (Hibiscus cannabinus L.) plants.

Withanage SP, Hossain MA, Kumar M S, Roslan HA, Abdullah MP, Napis SB, Shukor NA - Breed. Sci. (2015)

Effects of gibberellins on fiber length and diameter in transgenic kenaf and their respective UT plants. Length (a) and diameter (b) of bast and core fibers of transgenic G4 and V36 lines and their respective UT. Microscopic images of bast fiber length of the transgenic lines compared to their respective UT(c).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482167&req=5

f4-65_177: Effects of gibberellins on fiber length and diameter in transgenic kenaf and their respective UT plants. Length (a) and diameter (b) of bast and core fibers of transgenic G4 and V36 lines and their respective UT. Microscopic images of bast fiber length of the transgenic lines compared to their respective UT(c).
Mentions: The fiber length of the transgenic kenaf plant was significantly varied from the UT plant of both the varieties for bast, but not for the core. The mean bast fiber length of line G4-1 and G4-2 showed the highest values 2.80 mm compared to their UT plants (2.6 mm). The mean bast fiber length of V36-1 was 3.22 mm compared to 1.5 mm for their UT plants (Fig. 4a, c). The average length of the core fiber for all the transgenic lines (above 1 mm) was greater than that of the UT plants (around 0.8 mm) (Fig. 4a). Nonetheless, no significant (p ≤ 0.05) difference was observed in the fiber diameter of the transgenic lines from the UT line for bast and core (except the V36-1 in core) in both varieties although they were slightly higher to UT (Fig. 4b). In contrast to fiber diameter, the lumen diameter of all the transgenic lines in both G4 and V36 was comparatively lower for core than the UT plants but there was no significant difference in bast compared to UT (Fig. 5). The core lumen diameter of the transgenic lines of G4-1 ranged from 13.7 μm to 15.7 μm compared to 21.4 μm in the UT. All the transgenic lines showed higher cell wall thickness for both bast and core compared to their respective controls (Fig. 5). Accordingly, lines G4-1, G4-2, G4-4, G4-5, V36-1 and V36-13 had core cell wall thicknesses that were highly significant at p < 0.05. In the bast cell wall, lines G4-2, G4-5 and G4-7 showed significantly higher thicknesses whereas these were marginally different in other lines.

Bottom Line: Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene.The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition.Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia , 43400 UPM, Serdang, Selangor , Malaysia.

ABSTRACT
Kenaf (Hibiscus cannabinus L.; Family: Malvaceae), is multipurpose crop, one of the potential alternatives of natural fiber for biocomposite materials. Longer fiber and higher cellulose contents are required for good quality biocomposite materials. However, average length of kenaf fiber (2.6 mm in bast and 1.28 mm in whole plant) is below the critical length (4 mm) for biocomposite production. Present study describes whether fiber length and cellulose content of kenaf plants could be enhanced by increasing GA biosynthesis in plants by overexpressing Arabidopsis thaliana Gibberellic Acid 20 oxidase (AtGA20ox) gene. AtGA20ox gene with intron was overexpressed in kenaf plants under the control of double CaMV 35S promoter, followed by in planta transformation into V36 and G4 varieties of kenaf. The lines with higher levels of bioactive GA (0.3-1.52 ng g(-1) fresh weight) were further characterized for their morphological and biochemical traits including vegetative and reproductive growth, fiber dimension and chemical composition. Positive impact of increased gibberellins on biochemical composition, fiber dimension and their derivative values were demonstrated in some lines of transgenic kenaf including increased cellulose content (91%), fiber length and quality but it still requires further study to confirm the critical level of this particular bioactive GA in transgenic plants.

No MeSH data available.


Related in: MedlinePlus