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Healing activity of Casearia sylvestris Sw. in second-degree scald burns in rodents.

de Campos EP, Trombini LN, Rodrigues R, Portella DL, Werner AC, Ferraz MC, de Oliveira RV, Cogo JC, Oshima-Franco Y, Aranha N, Gerenutti M - BMC Res Notes (2015)

Bottom Line: Many of these victims have small size wounds and superficial partial thickness and do not seek specialized medical care.Significant differences were observed between the four groups studied considering: extension of the healing area, neovascularization, fibroblast proliferation, and epithelialization.The anti-inflammatory and bactericidal effects of C. sylvestris Sw. suggests a potential therapeutic benefit in the treatment of inflammatory conditions in second-degree scald burn injuries, as well as, counteracting against the in vitro paralysis induced by B. jararacussu venom.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for the Toxicological Research (Lapetox), Department of Pharmaceutical Sciences, University of Sorocaba (UNISO), Cidade Universitária, Rodovia Raposo Tavares km 92.5, 18023-000, Sorocaba, São Paulo, Brazil. ninoepd@hotmail.com.

ABSTRACT

Background: Every year thousands of people are victims of burns, mainly scald burns. Many of these victims have small size wounds and superficial partial thickness and do not seek specialized medical care. As in Brazil Casearia sylvestris Sw., popularly known as guaçatonga is widely used for its analgesic, antiseptic and anti-inflammatory activities, this study sought to evaluate the effects of its hydroalcoholic extract in healing process of burns injuries.

Methods: The obtained extract was validated applying a thin layer chromatography and sophisticated validation method using Bothrops jararacussu snake venom that is necrotic and inflammatory, and by which guaçatonga extract was able to neutralize the irreversible neuromuscular blockade induced by the venom. After induction of the scald injury, the animals were treated daily with saline solution spray; spray containing extract; biofilm; or biofilm impregnated with extract.

Results: Significant differences were observed between the four groups studied considering: extension of the healing area, neovascularization, fibroblast proliferation, and epithelialization.

Conclusion: The anti-inflammatory and bactericidal effects of C. sylvestris Sw. suggests a potential therapeutic benefit in the treatment of inflammatory conditions in second-degree scald burn injuries, as well as, counteracting against the in vitro paralysis induced by B. jararacussu venom.

No MeSH data available.


Related in: MedlinePlus

Chromatographic profile of hydroalcoholic (2) and methanol (3) extracts of Casearia sylvestris Sw. leaves by thin layer chromatography. Solvent system: acetone:chloroform:formic acid (10:75:8; v/v). Developer: NP/PEG; NP, diphenylboric acid 2-aminoethyl ester. PEG polyethylene glycol. Standards: 1 caffeic acid; 4 rutin.
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Fig1: Chromatographic profile of hydroalcoholic (2) and methanol (3) extracts of Casearia sylvestris Sw. leaves by thin layer chromatography. Solvent system: acetone:chloroform:formic acid (10:75:8; v/v). Developer: NP/PEG; NP, diphenylboric acid 2-aminoethyl ester. PEG polyethylene glycol. Standards: 1 caffeic acid; 4 rutin.

Mentions: Figure 1 shows the chromatoplaque of hydroalcoholic extract (2) in comparison to previously obtained methanolic extract (3) from C. sylvestris Sw. leaves. Phytochemical standards as caffeic acid (1) and rutin (4) only expressed caffeic acid, in reason of the applied solvent system. Note the similarity between hydroalcoholic and methanol extracts, showing that both extracts represent the same phytochemical substances.Figure 1


Healing activity of Casearia sylvestris Sw. in second-degree scald burns in rodents.

de Campos EP, Trombini LN, Rodrigues R, Portella DL, Werner AC, Ferraz MC, de Oliveira RV, Cogo JC, Oshima-Franco Y, Aranha N, Gerenutti M - BMC Res Notes (2015)

Chromatographic profile of hydroalcoholic (2) and methanol (3) extracts of Casearia sylvestris Sw. leaves by thin layer chromatography. Solvent system: acetone:chloroform:formic acid (10:75:8; v/v). Developer: NP/PEG; NP, diphenylboric acid 2-aminoethyl ester. PEG polyethylene glycol. Standards: 1 caffeic acid; 4 rutin.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4482163&req=5

Fig1: Chromatographic profile of hydroalcoholic (2) and methanol (3) extracts of Casearia sylvestris Sw. leaves by thin layer chromatography. Solvent system: acetone:chloroform:formic acid (10:75:8; v/v). Developer: NP/PEG; NP, diphenylboric acid 2-aminoethyl ester. PEG polyethylene glycol. Standards: 1 caffeic acid; 4 rutin.
Mentions: Figure 1 shows the chromatoplaque of hydroalcoholic extract (2) in comparison to previously obtained methanolic extract (3) from C. sylvestris Sw. leaves. Phytochemical standards as caffeic acid (1) and rutin (4) only expressed caffeic acid, in reason of the applied solvent system. Note the similarity between hydroalcoholic and methanol extracts, showing that both extracts represent the same phytochemical substances.Figure 1

Bottom Line: Many of these victims have small size wounds and superficial partial thickness and do not seek specialized medical care.Significant differences were observed between the four groups studied considering: extension of the healing area, neovascularization, fibroblast proliferation, and epithelialization.The anti-inflammatory and bactericidal effects of C. sylvestris Sw. suggests a potential therapeutic benefit in the treatment of inflammatory conditions in second-degree scald burn injuries, as well as, counteracting against the in vitro paralysis induced by B. jararacussu venom.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for the Toxicological Research (Lapetox), Department of Pharmaceutical Sciences, University of Sorocaba (UNISO), Cidade Universitária, Rodovia Raposo Tavares km 92.5, 18023-000, Sorocaba, São Paulo, Brazil. ninoepd@hotmail.com.

ABSTRACT

Background: Every year thousands of people are victims of burns, mainly scald burns. Many of these victims have small size wounds and superficial partial thickness and do not seek specialized medical care. As in Brazil Casearia sylvestris Sw., popularly known as guaçatonga is widely used for its analgesic, antiseptic and anti-inflammatory activities, this study sought to evaluate the effects of its hydroalcoholic extract in healing process of burns injuries.

Methods: The obtained extract was validated applying a thin layer chromatography and sophisticated validation method using Bothrops jararacussu snake venom that is necrotic and inflammatory, and by which guaçatonga extract was able to neutralize the irreversible neuromuscular blockade induced by the venom. After induction of the scald injury, the animals were treated daily with saline solution spray; spray containing extract; biofilm; or biofilm impregnated with extract.

Results: Significant differences were observed between the four groups studied considering: extension of the healing area, neovascularization, fibroblast proliferation, and epithelialization.

Conclusion: The anti-inflammatory and bactericidal effects of C. sylvestris Sw. suggests a potential therapeutic benefit in the treatment of inflammatory conditions in second-degree scald burn injuries, as well as, counteracting against the in vitro paralysis induced by B. jararacussu venom.

No MeSH data available.


Related in: MedlinePlus