Assessing the translational landscape of myogenic differentiation by ribosome profiling.
Bottom Line: The formation of skeletal muscles is associated with drastic changes in protein requirements known to be safeguarded by tight control of gene transcription and mRNA processing.Enrichment was also found for specific pathways known to regulate muscle biology.We identified 298 transcripts with a significant switch in TIS usage during myogenesis, which was not explained by alternative promoter usage, as profiled by DeepCAGE.
Affiliation: Department of Human Genetics, Leiden University Medical Center, Postzone S4-P, PO Box 9600, 2300 RC Leiden, The Netherlands.Show MeSH
Mentions: Footprints recovered after halting translation with harringtonin or cycloheximide mainly mapped to protein coding genes (Figure 2A, Supplementary Tables S4 and S5). Reads mapping to repetitive sequences, including contamination from ribosomal and transfer RNAs, are shown separately in Supplementary Table S3.
Affiliation: Department of Human Genetics, Leiden University Medical Center, Postzone S4-P, PO Box 9600, 2300 RC Leiden, The Netherlands.