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A new surface plasmon resonance immunosensor for triazine pesticide determination in bovine milk: a comparison with conventional amperometric and screen-printed immunodevices.

Tomassetti M, Martini E, Campanella L, Favero G, Sanzó G, Mazzei F - Sensors (Basel) (2015)

Bottom Line: For conventional amperometric and screen-printed devices, "competitive" assays were used; conversely, the SPR transduction technique allowed a "direct" measurement format to be used.Nevertheless the measurement time is much shorter and the measurement itself much easier to perform.Lastly several applications and recovery tests were carried out on bovine milk samples, before and after spiking, to check for triazine pesticides in the samples, obtaining satisfactory results.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Sapienza University of Rome, P. Le Aldo Moro 5, 00185 Rome, Italy. mauro.tomassetti@uniroma1.it.

ABSTRACT
A detailed comparison was made of the analytical features of a new Surface Plasmon Resonance (SPR) immunodevice for triazine pesticide determination with those of two other amperometric (conventional and screen-printed) immunosensors and the advantages and disadvantages of the SPR method were thoroughly investigated. For conventional amperometric and screen-printed devices, "competitive" assays were used; conversely, the SPR transduction technique allowed a "direct" measurement format to be used. As far as the main analytical data are concerned, the SPR method does not seem to offer substantial advantages. Nevertheless the measurement time is much shorter and the measurement itself much easier to perform. Lastly several applications and recovery tests were carried out on bovine milk samples, before and after spiking, to check for triazine pesticides in the samples, obtaining satisfactory results.

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(a) Behaviour of the conventional amperometric immunosensor response as a function of increasing atrazine concentration, using an H2O2 electrode as transducer and peroxidase enzyme as marker; (b) corresponding calibration curve and confidence interval for atrazine determination, obtained using a semilogarithmic scale.
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sensors-15-10255-f005: (a) Behaviour of the conventional amperometric immunosensor response as a function of increasing atrazine concentration, using an H2O2 electrode as transducer and peroxidase enzyme as marker; (b) corresponding calibration curve and confidence interval for atrazine determination, obtained using a semilogarithmic scale.

Mentions: In the case of the SPR device the calibration curve for atrazine (Figure 4) showed a linearity range of 1.0 × 10−7 to 1.5 × 10−6 M and with a LOD value of approximately 5 × 10−8 M. The response of the conventional amperometric device for increasing atrazine concentration and the relative calibration curve are displayed in Figure 5 and Figure 6, respectively.


A new surface plasmon resonance immunosensor for triazine pesticide determination in bovine milk: a comparison with conventional amperometric and screen-printed immunodevices.

Tomassetti M, Martini E, Campanella L, Favero G, Sanzó G, Mazzei F - Sensors (Basel) (2015)

(a) Behaviour of the conventional amperometric immunosensor response as a function of increasing atrazine concentration, using an H2O2 electrode as transducer and peroxidase enzyme as marker; (b) corresponding calibration curve and confidence interval for atrazine determination, obtained using a semilogarithmic scale.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4482009&req=5

sensors-15-10255-f005: (a) Behaviour of the conventional amperometric immunosensor response as a function of increasing atrazine concentration, using an H2O2 electrode as transducer and peroxidase enzyme as marker; (b) corresponding calibration curve and confidence interval for atrazine determination, obtained using a semilogarithmic scale.
Mentions: In the case of the SPR device the calibration curve for atrazine (Figure 4) showed a linearity range of 1.0 × 10−7 to 1.5 × 10−6 M and with a LOD value of approximately 5 × 10−8 M. The response of the conventional amperometric device for increasing atrazine concentration and the relative calibration curve are displayed in Figure 5 and Figure 6, respectively.

Bottom Line: For conventional amperometric and screen-printed devices, "competitive" assays were used; conversely, the SPR transduction technique allowed a "direct" measurement format to be used.Nevertheless the measurement time is much shorter and the measurement itself much easier to perform.Lastly several applications and recovery tests were carried out on bovine milk samples, before and after spiking, to check for triazine pesticides in the samples, obtaining satisfactory results.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Sapienza University of Rome, P. Le Aldo Moro 5, 00185 Rome, Italy. mauro.tomassetti@uniroma1.it.

ABSTRACT
A detailed comparison was made of the analytical features of a new Surface Plasmon Resonance (SPR) immunodevice for triazine pesticide determination with those of two other amperometric (conventional and screen-printed) immunosensors and the advantages and disadvantages of the SPR method were thoroughly investigated. For conventional amperometric and screen-printed devices, "competitive" assays were used; conversely, the SPR transduction technique allowed a "direct" measurement format to be used. As far as the main analytical data are concerned, the SPR method does not seem to offer substantial advantages. Nevertheless the measurement time is much shorter and the measurement itself much easier to perform. Lastly several applications and recovery tests were carried out on bovine milk samples, before and after spiking, to check for triazine pesticides in the samples, obtaining satisfactory results.

Show MeSH