Limits...
Surface plasmon resonance: a versatile technique for biosensor applications.

Nguyen HH, Park J, Kang S, Kim M - Sensors (Basel) (2015)

Bottom Line: Surface plasmon resonance (SPR) is a label-free detection method which has emerged during the last two decades as a suitable and reliable platform in clinical analysis for biomolecular interactions.Here we summarize the principles, provide examples, and illustrate the utility of SPR and SPRI through example applications from the biomedical, proteomics, genomics and bioengineering fields.In addition, SPR signal amplification strategies and surface functionalization are covered in the review.

View Article: PubMed Central - PubMed

Affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. hhiep86@kribb.re.kr.

ABSTRACT
Surface plasmon resonance (SPR) is a label-free detection method which has emerged during the last two decades as a suitable and reliable platform in clinical analysis for biomolecular interactions. The technique makes it possible to measure interactions in real-time with high sensitivity and without the need of labels. This review article discusses a wide range of applications in optical-based sensors using either surface plasmon resonance (SPR) or surface plasmon resonance imaging (SPRI). Here we summarize the principles, provide examples, and illustrate the utility of SPR and SPRI through example applications from the biomedical, proteomics, genomics and bioengineering fields. In addition, SPR signal amplification strategies and surface functionalization are covered in the review.

Show MeSH
Strategy for detecting SEB in buffer and stool using antibody-coated magnetic nanobeads. (A) Steps for processing samples with colloidal immunomagnetic beads (antibody-coated superparamagnetic nanobeads). Anti-SEB antibody-modified nanobeads were mixed with solutions containing the target antigen (SEB) prior to the SPR measurement; (B) SPR detection of staphylococcal enterotoxin B (SEB) in buffer (663 RIU), in stool (365 RIU), and unprocessed (7 RIU). Adapted from [115].
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4481982&req=5

sensors-15-10481-f008: Strategy for detecting SEB in buffer and stool using antibody-coated magnetic nanobeads. (A) Steps for processing samples with colloidal immunomagnetic beads (antibody-coated superparamagnetic nanobeads). Anti-SEB antibody-modified nanobeads were mixed with solutions containing the target antigen (SEB) prior to the SPR measurement; (B) SPR detection of staphylococcal enterotoxin B (SEB) in buffer (663 RIU), in stool (365 RIU), and unprocessed (7 RIU). Adapted from [115].

Mentions: The use of magnetic nanoparticles (MNPs) has a history of a few decades in bioseparation but not long in bioassays. These materials have attracted a great deal of attention due to their advantages, such as higher surface-to-volume ratio, minimum disturbance to attached biomolecules, faster binding rates, higher miscibility, and higher specificity. Compared to other plasmonic nanoparticles, MNPs are more cost effective. Thus, MNPs could facilitate SPR surface immobilization and sample precipitation by the use of a magnetic field [115]. In 2009, Soelbert et al. developed a method for the rapid and sensitive detection of Staphylococcal enterotoxin B (SEB) using antibody-coated magnetic nanobeads. The magnetic characteristics of the beads were utilized for SEB purification and concentration from real complex matrices before the samples were allowed to flow into their miniature SPR system (Figure 8). An LOD of 100 picograms/mL for SEB was achieved in both buffer and stool samples.


Surface plasmon resonance: a versatile technique for biosensor applications.

Nguyen HH, Park J, Kang S, Kim M - Sensors (Basel) (2015)

Strategy for detecting SEB in buffer and stool using antibody-coated magnetic nanobeads. (A) Steps for processing samples with colloidal immunomagnetic beads (antibody-coated superparamagnetic nanobeads). Anti-SEB antibody-modified nanobeads were mixed with solutions containing the target antigen (SEB) prior to the SPR measurement; (B) SPR detection of staphylococcal enterotoxin B (SEB) in buffer (663 RIU), in stool (365 RIU), and unprocessed (7 RIU). Adapted from [115].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4481982&req=5

sensors-15-10481-f008: Strategy for detecting SEB in buffer and stool using antibody-coated magnetic nanobeads. (A) Steps for processing samples with colloidal immunomagnetic beads (antibody-coated superparamagnetic nanobeads). Anti-SEB antibody-modified nanobeads were mixed with solutions containing the target antigen (SEB) prior to the SPR measurement; (B) SPR detection of staphylococcal enterotoxin B (SEB) in buffer (663 RIU), in stool (365 RIU), and unprocessed (7 RIU). Adapted from [115].
Mentions: The use of magnetic nanoparticles (MNPs) has a history of a few decades in bioseparation but not long in bioassays. These materials have attracted a great deal of attention due to their advantages, such as higher surface-to-volume ratio, minimum disturbance to attached biomolecules, faster binding rates, higher miscibility, and higher specificity. Compared to other plasmonic nanoparticles, MNPs are more cost effective. Thus, MNPs could facilitate SPR surface immobilization and sample precipitation by the use of a magnetic field [115]. In 2009, Soelbert et al. developed a method for the rapid and sensitive detection of Staphylococcal enterotoxin B (SEB) using antibody-coated magnetic nanobeads. The magnetic characteristics of the beads were utilized for SEB purification and concentration from real complex matrices before the samples were allowed to flow into their miniature SPR system (Figure 8). An LOD of 100 picograms/mL for SEB was achieved in both buffer and stool samples.

Bottom Line: Surface plasmon resonance (SPR) is a label-free detection method which has emerged during the last two decades as a suitable and reliable platform in clinical analysis for biomolecular interactions.Here we summarize the principles, provide examples, and illustrate the utility of SPR and SPRI through example applications from the biomedical, proteomics, genomics and bioengineering fields.In addition, SPR signal amplification strategies and surface functionalization are covered in the review.

View Article: PubMed Central - PubMed

Affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea. hhiep86@kribb.re.kr.

ABSTRACT
Surface plasmon resonance (SPR) is a label-free detection method which has emerged during the last two decades as a suitable and reliable platform in clinical analysis for biomolecular interactions. The technique makes it possible to measure interactions in real-time with high sensitivity and without the need of labels. This review article discusses a wide range of applications in optical-based sensors using either surface plasmon resonance (SPR) or surface plasmon resonance imaging (SPRI). Here we summarize the principles, provide examples, and illustrate the utility of SPR and SPRI through example applications from the biomedical, proteomics, genomics and bioengineering fields. In addition, SPR signal amplification strategies and surface functionalization are covered in the review.

Show MeSH