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ZnO Nanostructure-Based Intracellular Sensor.

Asif MH, Danielsson B, Willander M - Sensors (Basel) (2015)

Bottom Line: Recently ZnO has attracted much interest because of its usefulness for intracellular measurements of biochemical species by using its semiconducting, electrochemical, catalytic properties and for being biosafe and biocompatible.ZnO thus has a wide range of applications in optoelectronics, intracellular nanosensors, transducers, energy conversion and medical sciences.For intracellular measurements, the ZnO nanowires/nanorods were grown on the tip of a borosilicate glass capillary (0.7 µm in diameter) and functionalized with membranes or enzymes to produce intracellular selective metal ion or glucose sensors.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, COMSATS Institute of Information Technology, Lahore 54000, Pakistan. asifhassan@ciitlahore.edu.pk.

ABSTRACT
Recently ZnO has attracted much interest because of its usefulness for intracellular measurements of biochemical species by using its semiconducting, electrochemical, catalytic properties and for being biosafe and biocompatible. ZnO thus has a wide range of applications in optoelectronics, intracellular nanosensors, transducers, energy conversion and medical sciences. This review relates specifically to intracellular electrochemical (glucose and free metal ion) biosensors based on functionalized zinc oxide nanowires/nanorods. For intracellular measurements, the ZnO nanowires/nanorods were grown on the tip of a borosilicate glass capillary (0.7 µm in diameter) and functionalized with membranes or enzymes to produce intracellular selective metal ion or glucose sensors. Successful intracellular measurements were carried out using ZnO nanowires/nanorods grown on small tips for glucose and free metal ions using two types of cells, human fat cells and frog oocytes. The sensors in this study were used to detect real-time changes of metal ions and glucose across human fat cells and frog cells using changes in the electrochemical potential at the interface of the intracellular micro-environment. Such devices are helpful in explaining various intracellular processes involving ions and glucose.

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Intracellular K+ concentrations in Kv channel-expressing Xenopusoocytes measured with electrophysiological and K+-selective microelectrode methods. The Field emission scanning electron microscopy images of the K+-selective microelectrode before (a,b) and after intracellular measurements (c). Data points are expressed as mean values for control oocytes and oocytes injected with 50 nL of indicated test solution (d). Error bars show SE. n = 3–5) [26].
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sensors-15-11787-f012: Intracellular K+ concentrations in Kv channel-expressing Xenopusoocytes measured with electrophysiological and K+-selective microelectrode methods. The Field emission scanning electron microscopy images of the K+-selective microelectrode before (a,b) and after intracellular measurements (c). Data points are expressed as mean values for control oocytes and oocytes injected with 50 nL of indicated test solution (d). Error bars show SE. n = 3–5) [26].

Mentions: All the microelectrodes were investigated pre- and post-experimentally with scanning electron microscopy to make sure that the nanorods on the K+-selective microelectrodes were not dissolved (Figure 12).


ZnO Nanostructure-Based Intracellular Sensor.

Asif MH, Danielsson B, Willander M - Sensors (Basel) (2015)

Intracellular K+ concentrations in Kv channel-expressing Xenopusoocytes measured with electrophysiological and K+-selective microelectrode methods. The Field emission scanning electron microscopy images of the K+-selective microelectrode before (a,b) and after intracellular measurements (c). Data points are expressed as mean values for control oocytes and oocytes injected with 50 nL of indicated test solution (d). Error bars show SE. n = 3–5) [26].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4481971&req=5

sensors-15-11787-f012: Intracellular K+ concentrations in Kv channel-expressing Xenopusoocytes measured with electrophysiological and K+-selective microelectrode methods. The Field emission scanning electron microscopy images of the K+-selective microelectrode before (a,b) and after intracellular measurements (c). Data points are expressed as mean values for control oocytes and oocytes injected with 50 nL of indicated test solution (d). Error bars show SE. n = 3–5) [26].
Mentions: All the microelectrodes were investigated pre- and post-experimentally with scanning electron microscopy to make sure that the nanorods on the K+-selective microelectrodes were not dissolved (Figure 12).

Bottom Line: Recently ZnO has attracted much interest because of its usefulness for intracellular measurements of biochemical species by using its semiconducting, electrochemical, catalytic properties and for being biosafe and biocompatible.ZnO thus has a wide range of applications in optoelectronics, intracellular nanosensors, transducers, energy conversion and medical sciences.For intracellular measurements, the ZnO nanowires/nanorods were grown on the tip of a borosilicate glass capillary (0.7 µm in diameter) and functionalized with membranes or enzymes to produce intracellular selective metal ion or glucose sensors.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, COMSATS Institute of Information Technology, Lahore 54000, Pakistan. asifhassan@ciitlahore.edu.pk.

ABSTRACT
Recently ZnO has attracted much interest because of its usefulness for intracellular measurements of biochemical species by using its semiconducting, electrochemical, catalytic properties and for being biosafe and biocompatible. ZnO thus has a wide range of applications in optoelectronics, intracellular nanosensors, transducers, energy conversion and medical sciences. This review relates specifically to intracellular electrochemical (glucose and free metal ion) biosensors based on functionalized zinc oxide nanowires/nanorods. For intracellular measurements, the ZnO nanowires/nanorods were grown on the tip of a borosilicate glass capillary (0.7 µm in diameter) and functionalized with membranes or enzymes to produce intracellular selective metal ion or glucose sensors. Successful intracellular measurements were carried out using ZnO nanowires/nanorods grown on small tips for glucose and free metal ions using two types of cells, human fat cells and frog oocytes. The sensors in this study were used to detect real-time changes of metal ions and glucose across human fat cells and frog cells using changes in the electrochemical potential at the interface of the intracellular micro-environment. Such devices are helpful in explaining various intracellular processes involving ions and glucose.

Show MeSH