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Caffeine/sleep-deprivation interaction in mice produces complex memory effects.

Onaolapo OJ, Onaolapo AY, Akanmu MA, Olayiwola G - Ann Neurosci (2015)

Bottom Line: Sleep deprivation negatively impacts memory, causing deficits in memory processes.Of interest is any agent that can offset such deficits.Results were analysed using multi-factorial ANOVA followed by Tukey HSD test and expressed as mean ± S.E.M, with p values less than 0.05 were considered statistically significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, Oshogbo, Osun State, Nigeria ; Department of Pharmacology, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, Oshogbo, Osun State, Nigeria.

ABSTRACT

Background: Sleep deprivation negatively impacts memory, causing deficits in memory processes. Of interest is any agent that can offset such deficits. Mice were given varying doses of caffeine for 14 days and then deprived of sleep for 6 hours by the 'gentle handling' method. Memory was assessed using the Novel Object Recognition Test and Y maze alternation.

Purpose: The study was designed to ascertain the impact of varying doses of caffeine combined with total sleep-deprivation on spatial and non spatial memory in mice.

Methods: Adult Swiss Webster mice of both sexes were assigned to six groups viz., vehicle (distilled water), or one of five selected doses of caffeine (10, 20, 40, 80 and 120 mg/kg) for 14 days via the oral route. Open field novel object recognition test and Y maze spatial working memory tests were carried out on day 14. Results were analysed using multi-factorial ANOVA followed by Tukey HSD test and expressed as mean ± S.E.M, with p values less than 0.05 were considered statistically significant.

Results: Novel object recognition tests (NOR) revealed that pre-training and pre-test sleep deprivation and caffeine combination impaired non spatial and spatial memory in male and female mice.

Conclusion: The study shows the complex interactions with memory that may arise when total sleep deprivation is superimposed on caffeine administration.

No MeSH data available.


Related in: MedlinePlus

Effect of Caffeine on recognition index in non sleep deprived (NSD) and sleep deprived (SD) mice in the novel object recognition test. Each bar represents Mean ± S.E.M, *p<0.05 compared to vehicle, n = 6.
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fig_3: Effect of Caffeine on recognition index in non sleep deprived (NSD) and sleep deprived (SD) mice in the novel object recognition test. Each bar represents Mean ± S.E.M, *p<0.05 compared to vehicle, n = 6.

Mentions: Comparing within group effects in sleep deprived and non sleep deprived male and female mice respectively against vehicle (distilled water) showed statistically significant increase in the recognition index (RI) in NSD female mice at 10 (p = 0.000) 20 (p = 0.001), 40 (p = 0.002) and 80 (p = 0.000) mg/kg and a significant reduction in recognition index in NSD males at 10 (p = 0.003), 40 (p = 0.002), 80 (p = 0.000) and 120 (p = 0.000) mg/kg, in SD females recognition index increased significantly at 20 (p = 0.013)mg/kg and decreased significantly at 10 (p = 0.043), 80 (p = 0.000) and 120 (p = 0.001) mg/kg, SD males showed a significant reduction in recognition index at 120 (p = 0.000) mg/kg of caffeine compared to corresponding vehicle as shown in Figure 3.


Caffeine/sleep-deprivation interaction in mice produces complex memory effects.

Onaolapo OJ, Onaolapo AY, Akanmu MA, Olayiwola G - Ann Neurosci (2015)

Effect of Caffeine on recognition index in non sleep deprived (NSD) and sleep deprived (SD) mice in the novel object recognition test. Each bar represents Mean ± S.E.M, *p<0.05 compared to vehicle, n = 6.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4481547&req=5

fig_3: Effect of Caffeine on recognition index in non sleep deprived (NSD) and sleep deprived (SD) mice in the novel object recognition test. Each bar represents Mean ± S.E.M, *p<0.05 compared to vehicle, n = 6.
Mentions: Comparing within group effects in sleep deprived and non sleep deprived male and female mice respectively against vehicle (distilled water) showed statistically significant increase in the recognition index (RI) in NSD female mice at 10 (p = 0.000) 20 (p = 0.001), 40 (p = 0.002) and 80 (p = 0.000) mg/kg and a significant reduction in recognition index in NSD males at 10 (p = 0.003), 40 (p = 0.002), 80 (p = 0.000) and 120 (p = 0.000) mg/kg, in SD females recognition index increased significantly at 20 (p = 0.013)mg/kg and decreased significantly at 10 (p = 0.043), 80 (p = 0.000) and 120 (p = 0.001) mg/kg, SD males showed a significant reduction in recognition index at 120 (p = 0.000) mg/kg of caffeine compared to corresponding vehicle as shown in Figure 3.

Bottom Line: Sleep deprivation negatively impacts memory, causing deficits in memory processes.Of interest is any agent that can offset such deficits.Results were analysed using multi-factorial ANOVA followed by Tukey HSD test and expressed as mean ± S.E.M, with p values less than 0.05 were considered statistically significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, Oshogbo, Osun State, Nigeria ; Department of Pharmacology, Faculty of Basic Medical Sciences, College of Health Sciences, Ladoke Akintola University of Technology, Oshogbo, Osun State, Nigeria.

ABSTRACT

Background: Sleep deprivation negatively impacts memory, causing deficits in memory processes. Of interest is any agent that can offset such deficits. Mice were given varying doses of caffeine for 14 days and then deprived of sleep for 6 hours by the 'gentle handling' method. Memory was assessed using the Novel Object Recognition Test and Y maze alternation.

Purpose: The study was designed to ascertain the impact of varying doses of caffeine combined with total sleep-deprivation on spatial and non spatial memory in mice.

Methods: Adult Swiss Webster mice of both sexes were assigned to six groups viz., vehicle (distilled water), or one of five selected doses of caffeine (10, 20, 40, 80 and 120 mg/kg) for 14 days via the oral route. Open field novel object recognition test and Y maze spatial working memory tests were carried out on day 14. Results were analysed using multi-factorial ANOVA followed by Tukey HSD test and expressed as mean ± S.E.M, with p values less than 0.05 were considered statistically significant.

Results: Novel object recognition tests (NOR) revealed that pre-training and pre-test sleep deprivation and caffeine combination impaired non spatial and spatial memory in male and female mice.

Conclusion: The study shows the complex interactions with memory that may arise when total sleep deprivation is superimposed on caffeine administration.

No MeSH data available.


Related in: MedlinePlus