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Single-dose Toxicity of Water-soluble Ginseng Pharmacopuncture Injected Intramuscularly in Rats.

Yu J, Sun S, Lee K, Kwon K - J Pharmacopuncture (2015)

Bottom Line: No deaths were found in this single-dose toxicity test of intramuscular injections of GP, and no significant changes in the general conditions, body weights, hematological and biochemistry tests, and autopsies were observed.These results suggest that GP is relatively safe.Further studies, including a repeated toxicity test, are needed to provide more concrete evidence for the safety of GP.

View Article: PubMed Central - PubMed

Affiliation: Department of Sasang Constitutional Medicine, Sangji University College of Korean Medicine, Wonju, Korea.

ABSTRACT

Objectives: Radix Ginseng has been traditionally used as an adaptogen that acts on the adrenal cortex and stimulates or relaxes the nervous system to restore emotional and physical balance and to improve well-being in cases of degenerative disease and/or old age. Radix Ginseng has been used for a long time, but the safety of ginseng pharmacopuncture needs testing. This study was done to analyze the single-dose toxicity of water-soluble ginseng pharmacopuncture (GP) intramuscular injections in rats.

Methods: All experiments were performed at Biotoxtech, an institution authorized to perform non clinical studies under the regulations of Good Laboratory Practice (GLP). Each group contained 10 Sprague-Dawley rats, 5 males and 5 females. GP was prepared in a sterile room at the Korean Pharmacopuncture Institute under regulations of Good Manufacturing Practice (GMP). GP dosages were 0.1, 0.5 and 1.0 mL for the experimental groups; normal saline was administered to the control group. The animals general condition was examined daily for 14 days, and the rats were weighed on the starting day and at 3, 7 and 14 days after administration of the pharmacopuncture. Hematological and biochemistry tests and autopsies were done to test the toxicological effect of GP after 14 days. This study was performed with approval from the Institutional Animal Ethics Committee of Biotextech.

Results: No deaths were found in this single-dose toxicity test of intramuscular injections of GP, and no significant changes in the general conditions, body weights, hematological and biochemistry tests, and autopsies were observed. The local injection site showed no changes. Based on these results, the lethal dose was assumed to be over 1.0 mL/animal in both sexes.

Conclusion: These results suggest that GP is relatively safe. Further studies, including a repeated toxicity test, are needed to provide more concrete evidence for the safety of GP.

No MeSH data available.


Related in: MedlinePlus

Body weights in female Sprague-Dawley rats.
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Figure 003: Body weights in female Sprague-Dawley rats.

Mentions: In this study, no deaths occurred in experimental rats of either sex based on this result, the LD50 of GP was assumed to be over 1.0 mL/animal. In addition, no meaningful changes in the body weights or abnormalities in the general conditions of the rats were noticed (Tables 3,4, Figs. 2,3) Furthermore, no meaningful changes in the results of the hematological tests and the biochemical tests were found (Tables 5,6) The necropsy and histopathological findings showed no abnormalities (Tables 7,8, Figs. 4,5,6,7).


Single-dose Toxicity of Water-soluble Ginseng Pharmacopuncture Injected Intramuscularly in Rats.

Yu J, Sun S, Lee K, Kwon K - J Pharmacopuncture (2015)

Body weights in female Sprague-Dawley rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4481402&req=5

Figure 003: Body weights in female Sprague-Dawley rats.
Mentions: In this study, no deaths occurred in experimental rats of either sex based on this result, the LD50 of GP was assumed to be over 1.0 mL/animal. In addition, no meaningful changes in the body weights or abnormalities in the general conditions of the rats were noticed (Tables 3,4, Figs. 2,3) Furthermore, no meaningful changes in the results of the hematological tests and the biochemical tests were found (Tables 5,6) The necropsy and histopathological findings showed no abnormalities (Tables 7,8, Figs. 4,5,6,7).

Bottom Line: No deaths were found in this single-dose toxicity test of intramuscular injections of GP, and no significant changes in the general conditions, body weights, hematological and biochemistry tests, and autopsies were observed.These results suggest that GP is relatively safe.Further studies, including a repeated toxicity test, are needed to provide more concrete evidence for the safety of GP.

View Article: PubMed Central - PubMed

Affiliation: Department of Sasang Constitutional Medicine, Sangji University College of Korean Medicine, Wonju, Korea.

ABSTRACT

Objectives: Radix Ginseng has been traditionally used as an adaptogen that acts on the adrenal cortex and stimulates or relaxes the nervous system to restore emotional and physical balance and to improve well-being in cases of degenerative disease and/or old age. Radix Ginseng has been used for a long time, but the safety of ginseng pharmacopuncture needs testing. This study was done to analyze the single-dose toxicity of water-soluble ginseng pharmacopuncture (GP) intramuscular injections in rats.

Methods: All experiments were performed at Biotoxtech, an institution authorized to perform non clinical studies under the regulations of Good Laboratory Practice (GLP). Each group contained 10 Sprague-Dawley rats, 5 males and 5 females. GP was prepared in a sterile room at the Korean Pharmacopuncture Institute under regulations of Good Manufacturing Practice (GMP). GP dosages were 0.1, 0.5 and 1.0 mL for the experimental groups; normal saline was administered to the control group. The animals general condition was examined daily for 14 days, and the rats were weighed on the starting day and at 3, 7 and 14 days after administration of the pharmacopuncture. Hematological and biochemistry tests and autopsies were done to test the toxicological effect of GP after 14 days. This study was performed with approval from the Institutional Animal Ethics Committee of Biotextech.

Results: No deaths were found in this single-dose toxicity test of intramuscular injections of GP, and no significant changes in the general conditions, body weights, hematological and biochemistry tests, and autopsies were observed. The local injection site showed no changes. Based on these results, the lethal dose was assumed to be over 1.0 mL/animal in both sexes.

Conclusion: These results suggest that GP is relatively safe. Further studies, including a repeated toxicity test, are needed to provide more concrete evidence for the safety of GP.

No MeSH data available.


Related in: MedlinePlus