Limits...
Toxicity of Single-dose Intramuscular Injection of Samjeong Pharmacopuncture in Sprague-Dawley Rats.

Kwon K, Kim CY, Kim NK, Sun SH, Seo HS - J Pharmacopuncture (2015)

Bottom Line: The injection of SP had no effects on the general symptoms, body weights, results of the hematologic, and serum biochemical examinations, and necropsy findings.In local tolerance tests at the injection sites, mild inflammation was observed in the experimental group, but it did not appear to be a treatment related effect.Therefore, the clinical use of SP is thought to be safe.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Otolaryngology, and Dermatology, Korean Medicine Hospital, Pusan National University, Yangsan, Korea.

ABSTRACT

Objectives: This study was carried out in order to find both the single-dose intramuscular injection toxicity and the approximate lethal dose of samjeong pharmacopuncture (SP) in Sprague-Dawley (SD) rats.

Methods: The SD rats in this study were divided into four groups, one control group (1.0 mL/animal, normal saline) and three experimental groups (0.25, 0.5, and 1.0 mL/animal, SP). All groups consisted of five male and five female rats. SP was injected as a single-dose intramuscularly at the thigh. After the injection, general symptoms and weight were observed for 14 days. After the observations had ended, hematologic and serum biochemical examinations, necropsy and a local tolerance test at the injection site were performed. The experiments were carried out at the Good Laboratory Practice firm, Biotoxtech Co. (Cheongwon, Chungbuk). Animal experiments were approved by the Ethics Committee (Approval Number: 130379).

Results: No deaths occurred in any of the three experimental groups. The injection of SP had no effects on the general symptoms, body weights, results of the hematologic, and serum biochemical examinations, and necropsy findings. In local tolerance tests at the injection sites, mild inflammation was observed in the experimental group, but it did not appear to be a treatment related effect.

Conclusion: Under the conditions of this test, the results from the injection of SP suggest that the approximate lethal dose of SP is above 1.0 mL/animal for both male and female SD rats. Therefore, the clinical use of SP is thought to be safe.

No MeSH data available.


Related in: MedlinePlus

Body weights in male Sprague-Dawley rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4481400&req=5

Figure 001: Body weights in male Sprague-Dawley rats.

Mentions: During the observation period, no mortalities or abnormalities of clinical signs were found neither male or female rats in the control or experimental groups. No statistically significant changes in the weights of either male or female rats were observed between the control and the experimental groups (Figs. 1,2). Hematology and blood biochemistry data on both male and female rats in the control and the experimental groups showed no significant changes (Tables 2, 3, 4, 5). After necropsy, detailed visual inspections of the organs and tissues of both male and female SD rats showed no abnormal changes (Table 6). Histopathological findings for both male and female SD rats in the control and the experimental groups showed no abnormal changes (Table 7). In local tolerance tests at the injection sites, mild inflammation was observed in two male SD rats in experimental group 3, but the inflammation was found not to be a treatment related effect.


Toxicity of Single-dose Intramuscular Injection of Samjeong Pharmacopuncture in Sprague-Dawley Rats.

Kwon K, Kim CY, Kim NK, Sun SH, Seo HS - J Pharmacopuncture (2015)

Body weights in male Sprague-Dawley rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4481400&req=5

Figure 001: Body weights in male Sprague-Dawley rats.
Mentions: During the observation period, no mortalities or abnormalities of clinical signs were found neither male or female rats in the control or experimental groups. No statistically significant changes in the weights of either male or female rats were observed between the control and the experimental groups (Figs. 1,2). Hematology and blood biochemistry data on both male and female rats in the control and the experimental groups showed no significant changes (Tables 2, 3, 4, 5). After necropsy, detailed visual inspections of the organs and tissues of both male and female SD rats showed no abnormal changes (Table 6). Histopathological findings for both male and female SD rats in the control and the experimental groups showed no abnormal changes (Table 7). In local tolerance tests at the injection sites, mild inflammation was observed in two male SD rats in experimental group 3, but the inflammation was found not to be a treatment related effect.

Bottom Line: The injection of SP had no effects on the general symptoms, body weights, results of the hematologic, and serum biochemical examinations, and necropsy findings.In local tolerance tests at the injection sites, mild inflammation was observed in the experimental group, but it did not appear to be a treatment related effect.Therefore, the clinical use of SP is thought to be safe.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Otolaryngology, and Dermatology, Korean Medicine Hospital, Pusan National University, Yangsan, Korea.

ABSTRACT

Objectives: This study was carried out in order to find both the single-dose intramuscular injection toxicity and the approximate lethal dose of samjeong pharmacopuncture (SP) in Sprague-Dawley (SD) rats.

Methods: The SD rats in this study were divided into four groups, one control group (1.0 mL/animal, normal saline) and three experimental groups (0.25, 0.5, and 1.0 mL/animal, SP). All groups consisted of five male and five female rats. SP was injected as a single-dose intramuscularly at the thigh. After the injection, general symptoms and weight were observed for 14 days. After the observations had ended, hematologic and serum biochemical examinations, necropsy and a local tolerance test at the injection site were performed. The experiments were carried out at the Good Laboratory Practice firm, Biotoxtech Co. (Cheongwon, Chungbuk). Animal experiments were approved by the Ethics Committee (Approval Number: 130379).

Results: No deaths occurred in any of the three experimental groups. The injection of SP had no effects on the general symptoms, body weights, results of the hematologic, and serum biochemical examinations, and necropsy findings. In local tolerance tests at the injection sites, mild inflammation was observed in the experimental group, but it did not appear to be a treatment related effect.

Conclusion: Under the conditions of this test, the results from the injection of SP suggest that the approximate lethal dose of SP is above 1.0 mL/animal for both male and female SD rats. Therefore, the clinical use of SP is thought to be safe.

No MeSH data available.


Related in: MedlinePlus