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Single-dose Intravenous Toxicology Testing of Daebohwalryeok Pharmcopuncture in Sprague-Dawley Rats.

Sun SH, Park S, Jeong JJ, Lee KH, Yu JS, Seo HS, Kwon KR - J Pharmacopuncture (2015)

Bottom Line: Then, histopathological tests were performed.In addition, no treatment related general symptoms or necropsy abnormalities were observed.Histopathological results showed no DHRP related effects in the 20 mL/kg DHRP group for either male or female rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, College of Korean Medicine, Sangji University, Wonju, Korea.

ABSTRACT

Objectives: The aims of the study were to test the single-dose intravenous toxicity of Daebohwalryeok pharmacopuncture (DHRP) in Sprague-Dawley (SD) rats and to estimate the crude lethal dose.

Methods: The experiments were conducted at Biotoxtech Co., a Good Laboratory Practice (GLP) laboratory, according to the GLP regulation and were approved by the Institutional Animal Care and Use Committee of Biotoxtech Co. (Approval no: 110156). The rats were divided into three groups: DHRP was injected into the rats in the two test groups at doses of 10 mL/kg and 20 mL/kg, respectively, and normal saline solution was injected into the rats in the control group. Single doses of DHRP were injected intravenously into 6 week old SD rats (5 male and 5 female rats per group). General symptoms were observed and weights were measured during the 14 day observation period after the injection. After the observation period, necropsies were done. Then, histopathological tests were performed. Weight data were analyzed with a one-way analysis of variance (ANOVA) by using statistical analysis system (SAS, version 9.2).

Results: No deaths and no statistical significant weight changes were observed for either male or female SD rats in either the control or the test groups during the observation period. In addition, no treatment related general symptoms or necropsy abnormalities were observed. Histopathological results showed no DHRP related effects in the 20 mL/kg DHRP group for either male or female rats.

Conclusion: Under the conditions of this study, the results from single-dose intravenous injections of DHRP showed that estimated lethal doses for both male and female rats were above 20 mL/kg.

No MeSH data available.


Related in: MedlinePlus

Histopathological observations (hematoxylin & eosin staining × 200).
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Figure 003: Histopathological observations (hematoxylin & eosin staining × 200).

Mentions: As a result of necropsy, no macroscopic abnormalities were observed in the control and the test groups during the observation period (Table 4). No DHRP related effects were observed in the 20 mL/kg DHRP group for both male and female rats. Other findings that were observed in the kidneys and the livers in the control and the high dose groups, which were spontaneous and adventitious ones, were lesions commonly observed in age relevant SD rats and had no toxicological significance (Fig. 3, Table 5).


Single-dose Intravenous Toxicology Testing of Daebohwalryeok Pharmcopuncture in Sprague-Dawley Rats.

Sun SH, Park S, Jeong JJ, Lee KH, Yu JS, Seo HS, Kwon KR - J Pharmacopuncture (2015)

Histopathological observations (hematoxylin & eosin staining × 200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4481398&req=5

Figure 003: Histopathological observations (hematoxylin & eosin staining × 200).
Mentions: As a result of necropsy, no macroscopic abnormalities were observed in the control and the test groups during the observation period (Table 4). No DHRP related effects were observed in the 20 mL/kg DHRP group for both male and female rats. Other findings that were observed in the kidneys and the livers in the control and the high dose groups, which were spontaneous and adventitious ones, were lesions commonly observed in age relevant SD rats and had no toxicological significance (Fig. 3, Table 5).

Bottom Line: Then, histopathological tests were performed.In addition, no treatment related general symptoms or necropsy abnormalities were observed.Histopathological results showed no DHRP related effects in the 20 mL/kg DHRP group for either male or female rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, College of Korean Medicine, Sangji University, Wonju, Korea.

ABSTRACT

Objectives: The aims of the study were to test the single-dose intravenous toxicity of Daebohwalryeok pharmacopuncture (DHRP) in Sprague-Dawley (SD) rats and to estimate the crude lethal dose.

Methods: The experiments were conducted at Biotoxtech Co., a Good Laboratory Practice (GLP) laboratory, according to the GLP regulation and were approved by the Institutional Animal Care and Use Committee of Biotoxtech Co. (Approval no: 110156). The rats were divided into three groups: DHRP was injected into the rats in the two test groups at doses of 10 mL/kg and 20 mL/kg, respectively, and normal saline solution was injected into the rats in the control group. Single doses of DHRP were injected intravenously into 6 week old SD rats (5 male and 5 female rats per group). General symptoms were observed and weights were measured during the 14 day observation period after the injection. After the observation period, necropsies were done. Then, histopathological tests were performed. Weight data were analyzed with a one-way analysis of variance (ANOVA) by using statistical analysis system (SAS, version 9.2).

Results: No deaths and no statistical significant weight changes were observed for either male or female SD rats in either the control or the test groups during the observation period. In addition, no treatment related general symptoms or necropsy abnormalities were observed. Histopathological results showed no DHRP related effects in the 20 mL/kg DHRP group for either male or female rats.

Conclusion: Under the conditions of this study, the results from single-dose intravenous injections of DHRP showed that estimated lethal doses for both male and female rats were above 20 mL/kg.

No MeSH data available.


Related in: MedlinePlus