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The Anticancer Role of Capsaicin in Experimentallyinduced Lung Carcinogenesis.

Anandakumar P, Kamaraj S, Jagan S, Ramakrishnan G, Asokkumar S, Naveenkumar C, Raghunandhakumar S, Vanitha MK, Devaki T - J Pharmacopuncture (2015)

Bottom Line: Capsaicin (CAP) is the chief pungent principle found in the hot red peppers and the chili peppers that have long been used as spices, food additives and drugs.The above alterations in extracellular matrix components were effectively counteracted in benzo(a)pyrene along with CAP supplemented animals when compared to benzo(a) pyrene alone supplemented animals.The results of Masson's trichome staining for collagen and of, immunoblotting analyses of matrix metalloproteases 2 and 9 further supported the biochemical findings.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai, India.

ABSTRACT

Objectives: Capsaicin (CAP) is the chief pungent principle found in the hot red peppers and the chili peppers that have long been used as spices, food additives and drugs. This study investigated the anticancer potential of CAP through its ability to modify extracellular matrix components and proteases during mice lung carcinogenesis.

Methods: Swiss albino mice were treated with benzo(a) pyrene (50 mg/kg body weight dissolved in olive oil) orally twice a week for four successive weeks to induce lung cancer at the end of 14(th) week. CAP was administrated (10 mg/kg body weight dissolved in olive oil) intraperitoneally. Extracellular matrix components were assayed; Masson's trichome staining of lung tissues was performed. Western blot analyses of matrix metalloproteases 2 and 9 were also carried out.

Results: In comparison with the control animals, animals in which benzo(a)pyrene had induced lung cancer showed significant increases in extracellular matrix components such as collagen (hydroxy proline), elastin, uronic acid and hexosamine and in glycosaminoglycans such as hyaluronate, chondroitin sulfate, keratan sulfate and dermatan sulfate. The above alterations in extracellular matrix components were effectively counteracted in benzo(a)pyrene along with CAP supplemented animals when compared to benzo(a) pyrene alone supplemented animals. The results of Masson's trichome staining for collagen and of, immunoblotting analyses of matrix metalloproteases 2 and 9 further supported the biochemical findings.

Conclusion: The apparent potential of CAP in modulating extracellular matrix components and proteases suggests that CAP plays a chemomodulatory and anti- cancer role working against experimentally induced lung carcinogenesis.

No MeSH data available.


Related in: MedlinePlus

Levels of glycosaminoglycans in the lungs of the control and the experimental group of animals.
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Figure 003: Levels of glycosaminoglycans in the lungs of the control and the experimental group of animals.

Mentions: Fig. 3 depicts the levels of glycosaminoglycans in the control and the experimental groups of animals. Significant (P < 0.05) increase in the levels of hyaluronate, chondroitin sulfate, keratan sulfate and dermatan sulfate were noticed in the lung cancer bearing animals (group II) when compared with the group I control animals. Supplementation with CAP significantly (P < 0.05) restored the levels of these glycosaminoglycans to near normalcy in group IV animals when compared to group II animals.


The Anticancer Role of Capsaicin in Experimentallyinduced Lung Carcinogenesis.

Anandakumar P, Kamaraj S, Jagan S, Ramakrishnan G, Asokkumar S, Naveenkumar C, Raghunandhakumar S, Vanitha MK, Devaki T - J Pharmacopuncture (2015)

Levels of glycosaminoglycans in the lungs of the control and the experimental group of animals.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4481395&req=5

Figure 003: Levels of glycosaminoglycans in the lungs of the control and the experimental group of animals.
Mentions: Fig. 3 depicts the levels of glycosaminoglycans in the control and the experimental groups of animals. Significant (P < 0.05) increase in the levels of hyaluronate, chondroitin sulfate, keratan sulfate and dermatan sulfate were noticed in the lung cancer bearing animals (group II) when compared with the group I control animals. Supplementation with CAP significantly (P < 0.05) restored the levels of these glycosaminoglycans to near normalcy in group IV animals when compared to group II animals.

Bottom Line: Capsaicin (CAP) is the chief pungent principle found in the hot red peppers and the chili peppers that have long been used as spices, food additives and drugs.The above alterations in extracellular matrix components were effectively counteracted in benzo(a)pyrene along with CAP supplemented animals when compared to benzo(a) pyrene alone supplemented animals.The results of Masson's trichome staining for collagen and of, immunoblotting analyses of matrix metalloproteases 2 and 9 further supported the biochemical findings.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Madras, Guindy Campus, Chennai, India.

ABSTRACT

Objectives: Capsaicin (CAP) is the chief pungent principle found in the hot red peppers and the chili peppers that have long been used as spices, food additives and drugs. This study investigated the anticancer potential of CAP through its ability to modify extracellular matrix components and proteases during mice lung carcinogenesis.

Methods: Swiss albino mice were treated with benzo(a) pyrene (50 mg/kg body weight dissolved in olive oil) orally twice a week for four successive weeks to induce lung cancer at the end of 14(th) week. CAP was administrated (10 mg/kg body weight dissolved in olive oil) intraperitoneally. Extracellular matrix components were assayed; Masson's trichome staining of lung tissues was performed. Western blot analyses of matrix metalloproteases 2 and 9 were also carried out.

Results: In comparison with the control animals, animals in which benzo(a)pyrene had induced lung cancer showed significant increases in extracellular matrix components such as collagen (hydroxy proline), elastin, uronic acid and hexosamine and in glycosaminoglycans such as hyaluronate, chondroitin sulfate, keratan sulfate and dermatan sulfate. The above alterations in extracellular matrix components were effectively counteracted in benzo(a)pyrene along with CAP supplemented animals when compared to benzo(a) pyrene alone supplemented animals. The results of Masson's trichome staining for collagen and of, immunoblotting analyses of matrix metalloproteases 2 and 9 further supported the biochemical findings.

Conclusion: The apparent potential of CAP in modulating extracellular matrix components and proteases suggests that CAP plays a chemomodulatory and anti- cancer role working against experimentally induced lung carcinogenesis.

No MeSH data available.


Related in: MedlinePlus