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Dendritic planarity of Purkinje cells is independent of Reelin signaling.

Kim J, Park TJ, Kwon N, Lee D, Kim S, Kohmura Y, Ishikawa T, Kim KT, Curran T, Je JH - Brain Struct Funct (2014)

Bottom Line: Purkinje cells that failed to migrate completely exhibited conical dendrites with abnormal 3-D arborization and reduced dendritic complexity.In contrast, Purkinje cells that migrated successfully displayed planar dendritic and spine morphologies similar to normal cells, despite reduced dendritic complexity.While Reelin signaling is important for the migration process, it does not make a direct major contribution to dendrite formation.

View Article: PubMed Central - PubMed

Affiliation: X-ray Imaging Center, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.

ABSTRACT
The dendritic planarity of Purkinje cells is critical for cerebellar circuit formation. In the absence of Crk and CrkL, the Reelin pathway does not function resulting in partial Purkinje cell migration and defective dendritogenesis. However, the relationships among Purkinje cell migration, dendritic development and Reelin signaling have not been clearly delineated. Here, we use synchrotron X-ray microscopy to obtain 3-D images of Golgi-stained Purkinje cell dendrites. Purkinje cells that failed to migrate completely exhibited conical dendrites with abnormal 3-D arborization and reduced dendritic complexity. Furthermore, their spines were fewer in number with a distorted morphology. In contrast, Purkinje cells that migrated successfully displayed planar dendritic and spine morphologies similar to normal cells, despite reduced dendritic complexity. These results indicate that, during cerebellar formation, Purkinje cells migrate into an environment that supports development of dendritic planarity and spine formation. While Reelin signaling is important for the migration process, it does not make a direct major contribution to dendrite formation.

No MeSH data available.


Related in: MedlinePlus

3-D tomographic volume-rendered images of cerebellar tissues. a 3-D image of an entire cerebellum in a normal mouse (Movie S1). The lobular and PC layer structures are marked by the white and light green dashed lines, respectively. b 3-D magnified image of the white box region in panel a (Movie S2). PC and granule cells are marked by blue and magentaasterisks, respectively. The cerebellar layer arrangement is described by the white dashed lines. ml molecular layer; pcl PC layer, igl internal granular cell layer. c 3-D image of an entire cerebellum in a Crk/CrkL knockout mouse (Movie S3). Migrated and non-migrated PC are marked by the white and cyan blue boxes, respectively. d 3-D magnified image of the white box region in panel c (Movie S4). Migrated PC are marked by blue asterisks. ml molecular layer, pcl PC layer. e 3-D magnified image of the cyan blue box region in panel c (Movie S5). Non-migrated PC are marked by red asterisks
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Fig2: 3-D tomographic volume-rendered images of cerebellar tissues. a 3-D image of an entire cerebellum in a normal mouse (Movie S1). The lobular and PC layer structures are marked by the white and light green dashed lines, respectively. b 3-D magnified image of the white box region in panel a (Movie S2). PC and granule cells are marked by blue and magentaasterisks, respectively. The cerebellar layer arrangement is described by the white dashed lines. ml molecular layer; pcl PC layer, igl internal granular cell layer. c 3-D image of an entire cerebellum in a Crk/CrkL knockout mouse (Movie S3). Migrated and non-migrated PC are marked by the white and cyan blue boxes, respectively. d 3-D magnified image of the white box region in panel c (Movie S4). Migrated PC are marked by blue asterisks. ml molecular layer, pcl PC layer. e 3-D magnified image of the cyan blue box region in panel c (Movie S5). Non-migrated PC are marked by red asterisks

Mentions: We took advantage of microtomography of a Golgi-stained cerebellum to reveal its 3-D organization as well as details of PC dendritic arbors (Fig. 2). Figure 2a shows the typical folia (white dashed line) and PC layer (light green dashed line) characteristic of the normal cerebellum (Movie S1). The magnified view in Fig. 2b (boxed area in Fig. 2a) reveals the cerebellar layer arrangement (indicated by the dashed white lines) with PC (blue asterisk) and granule cells (magenta asterisk) (Movie S2). Highly branched PC were aligned in parallel in the PC plate. However, in mice lacking neuronal Crk and CrkL, two classes of PC were identified: those that failed to migrate to their final destinations (cyan blue box), and those that migrated to the PC plate (white box) (Fig. 2c). This is most apparent in the original 3-D image (Movie S3). We noticed that the migrated PC (blue asterisks) exhibited planar structures typical for PC dendrites (Fig. 2d; white box in Fig. 2c; Movie S4), but this was absent from non-migrated PC (red asterisk in Fig. 2e; cyan blue box in Fig. 2c; Movie S5). Thus, the two classes of PC were distinguished both by their location in the cerebellum and by the arrangement of their dendrites.Fig. 2


Dendritic planarity of Purkinje cells is independent of Reelin signaling.

Kim J, Park TJ, Kwon N, Lee D, Kim S, Kohmura Y, Ishikawa T, Kim KT, Curran T, Je JH - Brain Struct Funct (2014)

3-D tomographic volume-rendered images of cerebellar tissues. a 3-D image of an entire cerebellum in a normal mouse (Movie S1). The lobular and PC layer structures are marked by the white and light green dashed lines, respectively. b 3-D magnified image of the white box region in panel a (Movie S2). PC and granule cells are marked by blue and magentaasterisks, respectively. The cerebellar layer arrangement is described by the white dashed lines. ml molecular layer; pcl PC layer, igl internal granular cell layer. c 3-D image of an entire cerebellum in a Crk/CrkL knockout mouse (Movie S3). Migrated and non-migrated PC are marked by the white and cyan blue boxes, respectively. d 3-D magnified image of the white box region in panel c (Movie S4). Migrated PC are marked by blue asterisks. ml molecular layer, pcl PC layer. e 3-D magnified image of the cyan blue box region in panel c (Movie S5). Non-migrated PC are marked by red asterisks
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Fig2: 3-D tomographic volume-rendered images of cerebellar tissues. a 3-D image of an entire cerebellum in a normal mouse (Movie S1). The lobular and PC layer structures are marked by the white and light green dashed lines, respectively. b 3-D magnified image of the white box region in panel a (Movie S2). PC and granule cells are marked by blue and magentaasterisks, respectively. The cerebellar layer arrangement is described by the white dashed lines. ml molecular layer; pcl PC layer, igl internal granular cell layer. c 3-D image of an entire cerebellum in a Crk/CrkL knockout mouse (Movie S3). Migrated and non-migrated PC are marked by the white and cyan blue boxes, respectively. d 3-D magnified image of the white box region in panel c (Movie S4). Migrated PC are marked by blue asterisks. ml molecular layer, pcl PC layer. e 3-D magnified image of the cyan blue box region in panel c (Movie S5). Non-migrated PC are marked by red asterisks
Mentions: We took advantage of microtomography of a Golgi-stained cerebellum to reveal its 3-D organization as well as details of PC dendritic arbors (Fig. 2). Figure 2a shows the typical folia (white dashed line) and PC layer (light green dashed line) characteristic of the normal cerebellum (Movie S1). The magnified view in Fig. 2b (boxed area in Fig. 2a) reveals the cerebellar layer arrangement (indicated by the dashed white lines) with PC (blue asterisk) and granule cells (magenta asterisk) (Movie S2). Highly branched PC were aligned in parallel in the PC plate. However, in mice lacking neuronal Crk and CrkL, two classes of PC were identified: those that failed to migrate to their final destinations (cyan blue box), and those that migrated to the PC plate (white box) (Fig. 2c). This is most apparent in the original 3-D image (Movie S3). We noticed that the migrated PC (blue asterisks) exhibited planar structures typical for PC dendrites (Fig. 2d; white box in Fig. 2c; Movie S4), but this was absent from non-migrated PC (red asterisk in Fig. 2e; cyan blue box in Fig. 2c; Movie S5). Thus, the two classes of PC were distinguished both by their location in the cerebellum and by the arrangement of their dendrites.Fig. 2

Bottom Line: Purkinje cells that failed to migrate completely exhibited conical dendrites with abnormal 3-D arborization and reduced dendritic complexity.In contrast, Purkinje cells that migrated successfully displayed planar dendritic and spine morphologies similar to normal cells, despite reduced dendritic complexity.While Reelin signaling is important for the migration process, it does not make a direct major contribution to dendrite formation.

View Article: PubMed Central - PubMed

Affiliation: X-ray Imaging Center, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea.

ABSTRACT
The dendritic planarity of Purkinje cells is critical for cerebellar circuit formation. In the absence of Crk and CrkL, the Reelin pathway does not function resulting in partial Purkinje cell migration and defective dendritogenesis. However, the relationships among Purkinje cell migration, dendritic development and Reelin signaling have not been clearly delineated. Here, we use synchrotron X-ray microscopy to obtain 3-D images of Golgi-stained Purkinje cell dendrites. Purkinje cells that failed to migrate completely exhibited conical dendrites with abnormal 3-D arborization and reduced dendritic complexity. Furthermore, their spines were fewer in number with a distorted morphology. In contrast, Purkinje cells that migrated successfully displayed planar dendritic and spine morphologies similar to normal cells, despite reduced dendritic complexity. These results indicate that, during cerebellar formation, Purkinje cells migrate into an environment that supports development of dendritic planarity and spine formation. While Reelin signaling is important for the migration process, it does not make a direct major contribution to dendrite formation.

No MeSH data available.


Related in: MedlinePlus