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White matter tracts for the trafficking of neural progenitor cells characterized by cellular MRI and immunohistology: the role of CXCL12/CXCR4 signaling.

Chen CC, Hsu YH, Jayaseema DM, Chen JY, Hueng DY, Chang C - Brain Struct Funct (2014)

Bottom Line: To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs.It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm.The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts.

View Article: PubMed Central - PubMed

Affiliation: N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section 2, Academia Road, Nankang, Taipei, 11529, Taiwan.

ABSTRACT
White matter tracts are important for the trafficking of neural progenitor cells (NPCs) in both normal and pathological conditions, but the underlying mechanism is not clear. The directionality of white matter is advantageous for molecules or cells to distribute over a long distance, but this feature is unlikely solely responsible for efficient migration. The present study hypothesizes that the efficient migration of NPCs into white matter is under the influences of neurochemical attraction—CXCL12/CXCR4 signaling, a major mechanism underlying the targeted migration of NPCs. To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs. A living animal tracking platform based on MRI and a magnetic cell labeling technique was employed. The NPCs were magnetically labeled and then transplanted at the right end of the CC. CXCL12 was infused continuously at the left end. Migration of NPCs was monitored repeatedly over a 7-day course using 3D gradient echo T2*-weighted imaging. It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm. CXCL12 induced migration that was nine times as efficient in the speed. The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts. The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

No MeSH data available.


Related in: MedlinePlus

NPCs labeled with MPIO retained the ability to proliferate and differentiate. a No positive PB staining was observed in the unlabeled NPCs while numerous PB-stained particles were found in the cytoplasm of the MPIO-labeled NPCs. b MTT assays indicated a similar proliferation capacity between the labeled and unlabeled NPCs. c Differentiation of the unlabeled NPCs into astrocytes, oligodendrocytes, and neurons. d The labeled NPCs preserved the differentiation capacity. e The iron content in the labeled NPCs was stained by diaminobenzidine-enhanced PB. The results indicated that NPCs labeled with MPIO retained the ability to proliferate and differentiate
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Fig7: NPCs labeled with MPIO retained the ability to proliferate and differentiate. a No positive PB staining was observed in the unlabeled NPCs while numerous PB-stained particles were found in the cytoplasm of the MPIO-labeled NPCs. b MTT assays indicated a similar proliferation capacity between the labeled and unlabeled NPCs. c Differentiation of the unlabeled NPCs into astrocytes, oligodendrocytes, and neurons. d The labeled NPCs preserved the differentiation capacity. e The iron content in the labeled NPCs was stained by diaminobenzidine-enhanced PB. The results indicated that NPCs labeled with MPIO retained the ability to proliferate and differentiate

Mentions: As shown in Fig. 7a, numerous PB-stained particles were found in the cytoplasm in the labeled NPCs. In Fig. 7b, MTT assays indicated no significant difference in cell proliferation between the unlabeled and labeled NPCs. Differentiation was also examined. Both the unlabeled and labeled NPCs exhibited similar capacities for cellular differentiation as shown in Fig. 7c and d, respectively. The iron content of the labeled NPCs was stained by DAB-enhanced PB as shown in Fig. 7e. Note that MPIO labeling tended to obstruct the fluorescence shown in Fig. 7d, leading to a misimpression of weaker immunoreactivity in the labeled NPCs. The results indicate that NPCs labeled with MPIO retained the ability to proliferate and differentiate.Fig. 7


White matter tracts for the trafficking of neural progenitor cells characterized by cellular MRI and immunohistology: the role of CXCL12/CXCR4 signaling.

Chen CC, Hsu YH, Jayaseema DM, Chen JY, Hueng DY, Chang C - Brain Struct Funct (2014)

NPCs labeled with MPIO retained the ability to proliferate and differentiate. a No positive PB staining was observed in the unlabeled NPCs while numerous PB-stained particles were found in the cytoplasm of the MPIO-labeled NPCs. b MTT assays indicated a similar proliferation capacity between the labeled and unlabeled NPCs. c Differentiation of the unlabeled NPCs into astrocytes, oligodendrocytes, and neurons. d The labeled NPCs preserved the differentiation capacity. e The iron content in the labeled NPCs was stained by diaminobenzidine-enhanced PB. The results indicated that NPCs labeled with MPIO retained the ability to proliferate and differentiate
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4481304&req=5

Fig7: NPCs labeled with MPIO retained the ability to proliferate and differentiate. a No positive PB staining was observed in the unlabeled NPCs while numerous PB-stained particles were found in the cytoplasm of the MPIO-labeled NPCs. b MTT assays indicated a similar proliferation capacity between the labeled and unlabeled NPCs. c Differentiation of the unlabeled NPCs into astrocytes, oligodendrocytes, and neurons. d The labeled NPCs preserved the differentiation capacity. e The iron content in the labeled NPCs was stained by diaminobenzidine-enhanced PB. The results indicated that NPCs labeled with MPIO retained the ability to proliferate and differentiate
Mentions: As shown in Fig. 7a, numerous PB-stained particles were found in the cytoplasm in the labeled NPCs. In Fig. 7b, MTT assays indicated no significant difference in cell proliferation between the unlabeled and labeled NPCs. Differentiation was also examined. Both the unlabeled and labeled NPCs exhibited similar capacities for cellular differentiation as shown in Fig. 7c and d, respectively. The iron content of the labeled NPCs was stained by DAB-enhanced PB as shown in Fig. 7e. Note that MPIO labeling tended to obstruct the fluorescence shown in Fig. 7d, leading to a misimpression of weaker immunoreactivity in the labeled NPCs. The results indicate that NPCs labeled with MPIO retained the ability to proliferate and differentiate.Fig. 7

Bottom Line: To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs.It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm.The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts.

View Article: PubMed Central - PubMed

Affiliation: N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section 2, Academia Road, Nankang, Taipei, 11529, Taiwan.

ABSTRACT
White matter tracts are important for the trafficking of neural progenitor cells (NPCs) in both normal and pathological conditions, but the underlying mechanism is not clear. The directionality of white matter is advantageous for molecules or cells to distribute over a long distance, but this feature is unlikely solely responsible for efficient migration. The present study hypothesizes that the efficient migration of NPCs into white matter is under the influences of neurochemical attraction—CXCL12/CXCR4 signaling, a major mechanism underlying the targeted migration of NPCs. To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs. A living animal tracking platform based on MRI and a magnetic cell labeling technique was employed. The NPCs were magnetically labeled and then transplanted at the right end of the CC. CXCL12 was infused continuously at the left end. Migration of NPCs was monitored repeatedly over a 7-day course using 3D gradient echo T2*-weighted imaging. It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm. CXCL12 induced migration that was nine times as efficient in the speed. The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts. The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

No MeSH data available.


Related in: MedlinePlus