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White matter tracts for the trafficking of neural progenitor cells characterized by cellular MRI and immunohistology: the role of CXCL12/CXCR4 signaling.

Chen CC, Hsu YH, Jayaseema DM, Chen JY, Hueng DY, Chang C - Brain Struct Funct (2014)

Bottom Line: To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs.It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm.The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts.

View Article: PubMed Central - PubMed

Affiliation: N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section 2, Academia Road, Nankang, Taipei, 11529, Taiwan.

ABSTRACT
White matter tracts are important for the trafficking of neural progenitor cells (NPCs) in both normal and pathological conditions, but the underlying mechanism is not clear. The directionality of white matter is advantageous for molecules or cells to distribute over a long distance, but this feature is unlikely solely responsible for efficient migration. The present study hypothesizes that the efficient migration of NPCs into white matter is under the influences of neurochemical attraction—CXCL12/CXCR4 signaling, a major mechanism underlying the targeted migration of NPCs. To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs. A living animal tracking platform based on MRI and a magnetic cell labeling technique was employed. The NPCs were magnetically labeled and then transplanted at the right end of the CC. CXCL12 was infused continuously at the left end. Migration of NPCs was monitored repeatedly over a 7-day course using 3D gradient echo T2*-weighted imaging. It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm. CXCL12 induced migration that was nine times as efficient in the speed. The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts. The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

No MeSH data available.


Related in: MedlinePlus

Corroboration of the progenitor identity by nestin immunohistology. a The diagram of the infusion tube, migratory path, and graft. The areas nearby the graft and the migratory path marked by squares were photomicrographed. b A closer view of the migrating NPCs taken from the CXCL12-treated group. The NPCs were observed with both PB and nestin immunoreactivity. c The NPCs of the graft treated with CXCL12 infusion were positive for both nestin and PB. d Little migration occurred in the vehicle-treated group. e The graft view from the vehicle-treated group. Neither PB staining nor nestin immunoreactivity was observed beyond the graft site
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Fig6: Corroboration of the progenitor identity by nestin immunohistology. a The diagram of the infusion tube, migratory path, and graft. The areas nearby the graft and the migratory path marked by squares were photomicrographed. b A closer view of the migrating NPCs taken from the CXCL12-treated group. The NPCs were observed with both PB and nestin immunoreactivity. c The NPCs of the graft treated with CXCL12 infusion were positive for both nestin and PB. d Little migration occurred in the vehicle-treated group. e The graft view from the vehicle-treated group. Neither PB staining nor nestin immunoreactivity was observed beyond the graft site

Mentions: The image data (photomicrographs in Figs. 1a, c, 5, 6; MR images in Fig. 3) are from single representative animals from each of the groups, while the group-averaged data are shown in Figs. 1b and 4.Fig. 1


White matter tracts for the trafficking of neural progenitor cells characterized by cellular MRI and immunohistology: the role of CXCL12/CXCR4 signaling.

Chen CC, Hsu YH, Jayaseema DM, Chen JY, Hueng DY, Chang C - Brain Struct Funct (2014)

Corroboration of the progenitor identity by nestin immunohistology. a The diagram of the infusion tube, migratory path, and graft. The areas nearby the graft and the migratory path marked by squares were photomicrographed. b A closer view of the migrating NPCs taken from the CXCL12-treated group. The NPCs were observed with both PB and nestin immunoreactivity. c The NPCs of the graft treated with CXCL12 infusion were positive for both nestin and PB. d Little migration occurred in the vehicle-treated group. e The graft view from the vehicle-treated group. Neither PB staining nor nestin immunoreactivity was observed beyond the graft site
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4481304&req=5

Fig6: Corroboration of the progenitor identity by nestin immunohistology. a The diagram of the infusion tube, migratory path, and graft. The areas nearby the graft and the migratory path marked by squares were photomicrographed. b A closer view of the migrating NPCs taken from the CXCL12-treated group. The NPCs were observed with both PB and nestin immunoreactivity. c The NPCs of the graft treated with CXCL12 infusion were positive for both nestin and PB. d Little migration occurred in the vehicle-treated group. e The graft view from the vehicle-treated group. Neither PB staining nor nestin immunoreactivity was observed beyond the graft site
Mentions: The image data (photomicrographs in Figs. 1a, c, 5, 6; MR images in Fig. 3) are from single representative animals from each of the groups, while the group-averaged data are shown in Figs. 1b and 4.Fig. 1

Bottom Line: To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs.It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm.The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts.

View Article: PubMed Central - PubMed

Affiliation: N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section 2, Academia Road, Nankang, Taipei, 11529, Taiwan.

ABSTRACT
White matter tracts are important for the trafficking of neural progenitor cells (NPCs) in both normal and pathological conditions, but the underlying mechanism is not clear. The directionality of white matter is advantageous for molecules or cells to distribute over a long distance, but this feature is unlikely solely responsible for efficient migration. The present study hypothesizes that the efficient migration of NPCs into white matter is under the influences of neurochemical attraction—CXCL12/CXCR4 signaling, a major mechanism underlying the targeted migration of NPCs. To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs. A living animal tracking platform based on MRI and a magnetic cell labeling technique was employed. The NPCs were magnetically labeled and then transplanted at the right end of the CC. CXCL12 was infused continuously at the left end. Migration of NPCs was monitored repeatedly over a 7-day course using 3D gradient echo T2*-weighted imaging. It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm. CXCL12 induced migration that was nine times as efficient in the speed. The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts. The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

No MeSH data available.


Related in: MedlinePlus