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White matter tracts for the trafficking of neural progenitor cells characterized by cellular MRI and immunohistology: the role of CXCL12/CXCR4 signaling.

Chen CC, Hsu YH, Jayaseema DM, Chen JY, Hueng DY, Chang C - Brain Struct Funct (2014)

Bottom Line: It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm.The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts.The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

View Article: PubMed Central - PubMed

Affiliation: N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section 2, Academia Road, Nankang, Taipei, 11529, Taiwan.

ABSTRACT
White matter tracts are important for the trafficking of neural progenitor cells (NPCs) in both normal and pathological conditions, but the underlying mechanism is not clear. The directionality of white matter is advantageous for molecules or cells to distribute over a long distance, but this feature is unlikely solely responsible for efficient migration. The present study hypothesizes that the efficient migration of NPCs into white matter is under the influences of neurochemical attraction—CXCL12/CXCR4 signaling, a major mechanism underlying the targeted migration of NPCs. To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs. A living animal tracking platform based on MRI and a magnetic cell labeling technique was employed. The NPCs were magnetically labeled and then transplanted at the right end of the CC. CXCL12 was infused continuously at the left end. Migration of NPCs was monitored repeatedly over a 7-day course using 3D gradient echo T2*-weighted imaging. It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm. CXCL12 induced migration that was nine times as efficient in the speed. The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts. The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

No MeSH data available.


Related in: MedlinePlus

Measuring spontaneous and induced migration along the CC on cellular MRI. a In length, CXCL12 induced the NPCs to migrate with time over an extensive range from the grafted area. CXCL12-induced migration was nearly nine times as long as the vehicle-induced migration. b The averaged migration speeds were 269 ± 41 and 29 ± 45 μm/day for the CXCL12 and vehicle groups, respectively. c The migratory path increased in size with time. The size of CXCL12-induced migration was 28.7 times as large as vehicle-induced migration (i.e., spontaneous). d The average expansion rates from D0 to D7 were 115 ± 35 × 106 and 4 ± 4 × 106 μm3/day for the CXCL12 and vehicle groups, respectively
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Fig4: Measuring spontaneous and induced migration along the CC on cellular MRI. a In length, CXCL12 induced the NPCs to migrate with time over an extensive range from the grafted area. CXCL12-induced migration was nearly nine times as long as the vehicle-induced migration. b The averaged migration speeds were 269 ± 41 and 29 ± 45 μm/day for the CXCL12 and vehicle groups, respectively. c The migratory path increased in size with time. The size of CXCL12-induced migration was 28.7 times as large as vehicle-induced migration (i.e., spontaneous). d The average expansion rates from D0 to D7 were 115 ± 35 × 106 and 4 ± 4 × 106 μm3/day for the CXCL12 and vehicle groups, respectively

Mentions: The image data (photomicrographs in Figs. 1a, c, 5, 6; MR images in Fig. 3) are from single representative animals from each of the groups, while the group-averaged data are shown in Figs. 1b and 4.Fig. 1


White matter tracts for the trafficking of neural progenitor cells characterized by cellular MRI and immunohistology: the role of CXCL12/CXCR4 signaling.

Chen CC, Hsu YH, Jayaseema DM, Chen JY, Hueng DY, Chang C - Brain Struct Funct (2014)

Measuring spontaneous and induced migration along the CC on cellular MRI. a In length, CXCL12 induced the NPCs to migrate with time over an extensive range from the grafted area. CXCL12-induced migration was nearly nine times as long as the vehicle-induced migration. b The averaged migration speeds were 269 ± 41 and 29 ± 45 μm/day for the CXCL12 and vehicle groups, respectively. c The migratory path increased in size with time. The size of CXCL12-induced migration was 28.7 times as large as vehicle-induced migration (i.e., spontaneous). d The average expansion rates from D0 to D7 were 115 ± 35 × 106 and 4 ± 4 × 106 μm3/day for the CXCL12 and vehicle groups, respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4481304&req=5

Fig4: Measuring spontaneous and induced migration along the CC on cellular MRI. a In length, CXCL12 induced the NPCs to migrate with time over an extensive range from the grafted area. CXCL12-induced migration was nearly nine times as long as the vehicle-induced migration. b The averaged migration speeds were 269 ± 41 and 29 ± 45 μm/day for the CXCL12 and vehicle groups, respectively. c The migratory path increased in size with time. The size of CXCL12-induced migration was 28.7 times as large as vehicle-induced migration (i.e., spontaneous). d The average expansion rates from D0 to D7 were 115 ± 35 × 106 and 4 ± 4 × 106 μm3/day for the CXCL12 and vehicle groups, respectively
Mentions: The image data (photomicrographs in Figs. 1a, c, 5, 6; MR images in Fig. 3) are from single representative animals from each of the groups, while the group-averaged data are shown in Figs. 1b and 4.Fig. 1

Bottom Line: It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm.The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts.The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

View Article: PubMed Central - PubMed

Affiliation: N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section 2, Academia Road, Nankang, Taipei, 11529, Taiwan.

ABSTRACT
White matter tracts are important for the trafficking of neural progenitor cells (NPCs) in both normal and pathological conditions, but the underlying mechanism is not clear. The directionality of white matter is advantageous for molecules or cells to distribute over a long distance, but this feature is unlikely solely responsible for efficient migration. The present study hypothesizes that the efficient migration of NPCs into white matter is under the influences of neurochemical attraction—CXCL12/CXCR4 signaling, a major mechanism underlying the targeted migration of NPCs. To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs. A living animal tracking platform based on MRI and a magnetic cell labeling technique was employed. The NPCs were magnetically labeled and then transplanted at the right end of the CC. CXCL12 was infused continuously at the left end. Migration of NPCs was monitored repeatedly over a 7-day course using 3D gradient echo T2*-weighted imaging. It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm. CXCL12 induced migration that was nine times as efficient in the speed. The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts. The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

No MeSH data available.


Related in: MedlinePlus