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White matter tracts for the trafficking of neural progenitor cells characterized by cellular MRI and immunohistology: the role of CXCL12/CXCR4 signaling.

Chen CC, Hsu YH, Jayaseema DM, Chen JY, Hueng DY, Chang C - Brain Struct Funct (2014)

Bottom Line: To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs.It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm.The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts.

View Article: PubMed Central - PubMed

Affiliation: N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section 2, Academia Road, Nankang, Taipei, 11529, Taiwan.

ABSTRACT
White matter tracts are important for the trafficking of neural progenitor cells (NPCs) in both normal and pathological conditions, but the underlying mechanism is not clear. The directionality of white matter is advantageous for molecules or cells to distribute over a long distance, but this feature is unlikely solely responsible for efficient migration. The present study hypothesizes that the efficient migration of NPCs into white matter is under the influences of neurochemical attraction—CXCL12/CXCR4 signaling, a major mechanism underlying the targeted migration of NPCs. To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs. A living animal tracking platform based on MRI and a magnetic cell labeling technique was employed. The NPCs were magnetically labeled and then transplanted at the right end of the CC. CXCL12 was infused continuously at the left end. Migration of NPCs was monitored repeatedly over a 7-day course using 3D gradient echo T2*-weighted imaging. It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm. CXCL12 induced migration that was nine times as efficient in the speed. The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts. The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

No MeSH data available.


Related in: MedlinePlus

CXCR4 expression in NPCs verified by flow cytometry. a Gating for positive or non-fluorescence was done on unstained NPCs. The levels of the fluorescence were minimal. b The majority of the NPCs stained with the CXCR4 antibody and 7AAD fell within Q4 (CXCR4+/7AAD−). c More than 95 % of the NPCs were viable and CXCR4 positive. The values are expressed as mean ± standard deviation
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Fig2: CXCR4 expression in NPCs verified by flow cytometry. a Gating for positive or non-fluorescence was done on unstained NPCs. The levels of the fluorescence were minimal. b The majority of the NPCs stained with the CXCR4 antibody and 7AAD fell within Q4 (CXCR4+/7AAD−). c More than 95 % of the NPCs were viable and CXCR4 positive. The values are expressed as mean ± standard deviation

Mentions: The NPCs were stained by the antibody against CXCR4 and 7-AAD as the viability marker. Gating was done on unstained NPCs as shown in Fig. 2a. This generated four divisions as denoted. Figure 2b shows that the majority of the stained NPCs fell within Q4 (CXCR4+/7AAD−). The viable, CXCR4-positive population was more than 95 % as shown in Fig. 2c.Fig. 2


White matter tracts for the trafficking of neural progenitor cells characterized by cellular MRI and immunohistology: the role of CXCL12/CXCR4 signaling.

Chen CC, Hsu YH, Jayaseema DM, Chen JY, Hueng DY, Chang C - Brain Struct Funct (2014)

CXCR4 expression in NPCs verified by flow cytometry. a Gating for positive or non-fluorescence was done on unstained NPCs. The levels of the fluorescence were minimal. b The majority of the NPCs stained with the CXCR4 antibody and 7AAD fell within Q4 (CXCR4+/7AAD−). c More than 95 % of the NPCs were viable and CXCR4 positive. The values are expressed as mean ± standard deviation
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4481304&req=5

Fig2: CXCR4 expression in NPCs verified by flow cytometry. a Gating for positive or non-fluorescence was done on unstained NPCs. The levels of the fluorescence were minimal. b The majority of the NPCs stained with the CXCR4 antibody and 7AAD fell within Q4 (CXCR4+/7AAD−). c More than 95 % of the NPCs were viable and CXCR4 positive. The values are expressed as mean ± standard deviation
Mentions: The NPCs were stained by the antibody against CXCR4 and 7-AAD as the viability marker. Gating was done on unstained NPCs as shown in Fig. 2a. This generated four divisions as denoted. Figure 2b shows that the majority of the stained NPCs fell within Q4 (CXCR4+/7AAD−). The viable, CXCR4-positive population was more than 95 % as shown in Fig. 2c.Fig. 2

Bottom Line: To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs.It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm.The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts.

View Article: PubMed Central - PubMed

Affiliation: N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section 2, Academia Road, Nankang, Taipei, 11529, Taiwan.

ABSTRACT
White matter tracts are important for the trafficking of neural progenitor cells (NPCs) in both normal and pathological conditions, but the underlying mechanism is not clear. The directionality of white matter is advantageous for molecules or cells to distribute over a long distance, but this feature is unlikely solely responsible for efficient migration. The present study hypothesizes that the efficient migration of NPCs into white matter is under the influences of neurochemical attraction—CXCL12/CXCR4 signaling, a major mechanism underlying the targeted migration of NPCs. To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs. A living animal tracking platform based on MRI and a magnetic cell labeling technique was employed. The NPCs were magnetically labeled and then transplanted at the right end of the CC. CXCL12 was infused continuously at the left end. Migration of NPCs was monitored repeatedly over a 7-day course using 3D gradient echo T2*-weighted imaging. It was found that, CXCL12 induced NPCs to migrate up to 1,881 μm from the graft whereas the spontaneous migration was mere 200 μm. CXCL12 induced migration that was nine times as efficient in the speed. The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts. The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders.

No MeSH data available.


Related in: MedlinePlus