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CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer.

Nomura A, Banerjee S, Chugh R, Dudeja V, Yamamoto M, Vickers SM, Saluja AK - Oncotarget (2015)

Bottom Line: Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion.EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects.This study showed that CD133 expression contributes to pancreatic cancer "stemness," tumorigenicity, EMT induction, invasion, and metastasis.

View Article: PubMed Central - PubMed

Affiliation: Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis, MN, USA.

ABSTRACT
CD133 has been implicated as a cancer stem cell (CSC) surface marker in several malignancies including pancreatic cancer. However, the functional role of this surface glycoprotein in the cancer stem cell remains elusive. In this study, we determined that CD133 overexpression induced "stemness" properties in MIA-PaCa2 cells along with increased tumorigenicity, tumor progression, and metastasis in vivo. Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion. EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects. This study showed that CD133 expression contributes to pancreatic cancer "stemness," tumorigenicity, EMT induction, invasion, and metastasis.

No MeSH data available.


Related in: MedlinePlus

CD133 expression increases tumor formation(A) Representative images of tumor formation in MIA, EV-MIA, and CD133hi-MIA groups. (B) Tumor growth expressed in volume of tumors derived from EV-MIA and CD133hi-MIA groups.
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Figure 2: CD133 expression increases tumor formation(A) Representative images of tumor formation in MIA, EV-MIA, and CD133hi-MIA groups. (B) Tumor growth expressed in volume of tumors derived from EV-MIA and CD133hi-MIA groups.

Mentions: To determine whether CD133 surface expression determined the ability of a cancer cell to initiate tumors, we overexpressed CD133 (CD133hi-MIA) and the empty vector plasmid (EV-MIA) in MIA-PaCa2 cells. Different numbers of these cells were injected subcutaneously into athymic mice. Only ten CD133hi-MIA cells were needed to form tumors in 7/16 mice or 1,000 cells in 10/16 mice within 53 days, as compared with the same number in MIA PaCa-2 (0/16) or EV-MIA (2/16) controls (Table 1 and Figure 2A).


CD133 initiates tumors, induces epithelial-mesenchymal transition and increases metastasis in pancreatic cancer.

Nomura A, Banerjee S, Chugh R, Dudeja V, Yamamoto M, Vickers SM, Saluja AK - Oncotarget (2015)

CD133 expression increases tumor formation(A) Representative images of tumor formation in MIA, EV-MIA, and CD133hi-MIA groups. (B) Tumor growth expressed in volume of tumors derived from EV-MIA and CD133hi-MIA groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4480754&req=5

Figure 2: CD133 expression increases tumor formation(A) Representative images of tumor formation in MIA, EV-MIA, and CD133hi-MIA groups. (B) Tumor growth expressed in volume of tumors derived from EV-MIA and CD133hi-MIA groups.
Mentions: To determine whether CD133 surface expression determined the ability of a cancer cell to initiate tumors, we overexpressed CD133 (CD133hi-MIA) and the empty vector plasmid (EV-MIA) in MIA-PaCa2 cells. Different numbers of these cells were injected subcutaneously into athymic mice. Only ten CD133hi-MIA cells were needed to form tumors in 7/16 mice or 1,000 cells in 10/16 mice within 53 days, as compared with the same number in MIA PaCa-2 (0/16) or EV-MIA (2/16) controls (Table 1 and Figure 2A).

Bottom Line: Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion.EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects.This study showed that CD133 expression contributes to pancreatic cancer "stemness," tumorigenicity, EMT induction, invasion, and metastasis.

View Article: PubMed Central - PubMed

Affiliation: Division of Basic and Translational Research, Department of Surgery, University of Minnesota, Minneapolis, MN, USA.

ABSTRACT
CD133 has been implicated as a cancer stem cell (CSC) surface marker in several malignancies including pancreatic cancer. However, the functional role of this surface glycoprotein in the cancer stem cell remains elusive. In this study, we determined that CD133 overexpression induced "stemness" properties in MIA-PaCa2 cells along with increased tumorigenicity, tumor progression, and metastasis in vivo. Additionally, CD133 expression induced epithelial-mesenchymal transition (EMT) and increased in vitro invasion. EMT induction and increased invasiveness were mediated by NF-κB activation, as inhibition of NF-κB mitigated these effects. This study showed that CD133 expression contributes to pancreatic cancer "stemness," tumorigenicity, EMT induction, invasion, and metastasis.

No MeSH data available.


Related in: MedlinePlus