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MicroRNA-200a suppresses metastatic potential of side population cells in human hepatocellular carcinoma by decreasing ZEB2.

Yang X, Wang J, Qu S, Zhang H, Ruan B, Gao Y, Ma B, Wang X, Wu N, Li X, Dou K, Li H - Oncotarget (2015)

Bottom Line: We found that miR-200a was downregulated in HCC/SP and this was associated metastasis.Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2.The associations between miR-200a, SP cells and ZEB2 were validated in HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military Medical Uiversity, Xi'an, China.

ABSTRACT
Although microRNA-200a (miR-200a) is frequently downregulated in cancer, its role in side population (SP) has not been investigated. In this study, 101 pairs of primary hepatocellular carcinoma (HCC) tissues and matched normal control tissues were analyzed for miR-200a expression and its clinicopathological value was determined. We found that miR-200a was downregulated in HCC/SP and this was associated metastasis. MiR-200a suppressed metastasis of SP cells. Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2. The associations between miR-200a, SP cells and ZEB2 were validated in HCC. These findings reveal that miR-200a suppresses metastasis of SP cells by downregulating ZEB2.

No MeSH data available.


Related in: MedlinePlus

The correlation between miR-200a expression and EMT(A) An immunohistochemical analysis of miR-200a, ZEB2, and E-cadherin expression in HCC tissues and in adjacent non-tumor tissues. (B) The association between the expression of miR-200a and either ZEB2 or E-cadherin in human patients with HCC.
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Figure 2: The correlation between miR-200a expression and EMT(A) An immunohistochemical analysis of miR-200a, ZEB2, and E-cadherin expression in HCC tissues and in adjacent non-tumor tissues. (B) The association between the expression of miR-200a and either ZEB2 or E-cadherin in human patients with HCC.

Mentions: In our previous studies, we found that miR-200a inhibits the EMT process via the activation of transcriptional factors such as ZEB2, which is required during EMT; this has been thoroughly demonstrated in rat hepatic oval cells [22]. We further investigated the possible relationship between miR-200a, ZEB2 and E-cadherin expression in human HCC tissues. Immunohistochemistry revealed that miR-200a expression was inversely correlated with ZEB2 expression but was positively correlated with E-cadherin expression (Fig. 2A,B).


MicroRNA-200a suppresses metastatic potential of side population cells in human hepatocellular carcinoma by decreasing ZEB2.

Yang X, Wang J, Qu S, Zhang H, Ruan B, Gao Y, Ma B, Wang X, Wu N, Li X, Dou K, Li H - Oncotarget (2015)

The correlation between miR-200a expression and EMT(A) An immunohistochemical analysis of miR-200a, ZEB2, and E-cadherin expression in HCC tissues and in adjacent non-tumor tissues. (B) The association between the expression of miR-200a and either ZEB2 or E-cadherin in human patients with HCC.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4480725&req=5

Figure 2: The correlation between miR-200a expression and EMT(A) An immunohistochemical analysis of miR-200a, ZEB2, and E-cadherin expression in HCC tissues and in adjacent non-tumor tissues. (B) The association between the expression of miR-200a and either ZEB2 or E-cadherin in human patients with HCC.
Mentions: In our previous studies, we found that miR-200a inhibits the EMT process via the activation of transcriptional factors such as ZEB2, which is required during EMT; this has been thoroughly demonstrated in rat hepatic oval cells [22]. We further investigated the possible relationship between miR-200a, ZEB2 and E-cadherin expression in human HCC tissues. Immunohistochemistry revealed that miR-200a expression was inversely correlated with ZEB2 expression but was positively correlated with E-cadherin expression (Fig. 2A,B).

Bottom Line: We found that miR-200a was downregulated in HCC/SP and this was associated metastasis.Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2.The associations between miR-200a, SP cells and ZEB2 were validated in HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military Medical Uiversity, Xi'an, China.

ABSTRACT
Although microRNA-200a (miR-200a) is frequently downregulated in cancer, its role in side population (SP) has not been investigated. In this study, 101 pairs of primary hepatocellular carcinoma (HCC) tissues and matched normal control tissues were analyzed for miR-200a expression and its clinicopathological value was determined. We found that miR-200a was downregulated in HCC/SP and this was associated metastasis. MiR-200a suppressed metastasis of SP cells. Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2. The associations between miR-200a, SP cells and ZEB2 were validated in HCC. These findings reveal that miR-200a suppresses metastasis of SP cells by downregulating ZEB2.

No MeSH data available.


Related in: MedlinePlus