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MicroRNA-200a suppresses metastatic potential of side population cells in human hepatocellular carcinoma by decreasing ZEB2.

Yang X, Wang J, Qu S, Zhang H, Ruan B, Gao Y, Ma B, Wang X, Wu N, Li X, Dou K, Li H - Oncotarget (2015)

Bottom Line: We found that miR-200a was downregulated in HCC/SP and this was associated metastasis.Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2.The associations between miR-200a, SP cells and ZEB2 were validated in HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military Medical Uiversity, Xi'an, China.

ABSTRACT
Although microRNA-200a (miR-200a) is frequently downregulated in cancer, its role in side population (SP) has not been investigated. In this study, 101 pairs of primary hepatocellular carcinoma (HCC) tissues and matched normal control tissues were analyzed for miR-200a expression and its clinicopathological value was determined. We found that miR-200a was downregulated in HCC/SP and this was associated metastasis. MiR-200a suppressed metastasis of SP cells. Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2. The associations between miR-200a, SP cells and ZEB2 were validated in HCC. These findings reveal that miR-200a suppresses metastasis of SP cells by downregulating ZEB2.

No MeSH data available.


Related in: MedlinePlus

MiR-200a is down-regulated in HCC(A) The expression of miR-200a in HCC tissue specimens and in corresponding non-tumor tissues was measured by qRT-PCR. (B) The expression status of miR-200a in 4 human HCC cell lines and one normal human hepatocyte was measured by qRT-PCR. (C) The relative expression level of miR-200a in primary tumor samples with or without clinically confirmed metastasis was measured by qRT-PCR. (D) Kaplan-Meier analysis of the correlation between miR-200a expression and the overall survival of 101 patients with HCC. Patients in the low miR-200a expression group had a significantly shorter overall survival (P < 0.01); * P < 0.05, t test.
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Figure 1: MiR-200a is down-regulated in HCC(A) The expression of miR-200a in HCC tissue specimens and in corresponding non-tumor tissues was measured by qRT-PCR. (B) The expression status of miR-200a in 4 human HCC cell lines and one normal human hepatocyte was measured by qRT-PCR. (C) The relative expression level of miR-200a in primary tumor samples with or without clinically confirmed metastasis was measured by qRT-PCR. (D) Kaplan-Meier analysis of the correlation between miR-200a expression and the overall survival of 101 patients with HCC. Patients in the low miR-200a expression group had a significantly shorter overall survival (P < 0.01); * P < 0.05, t test.

Mentions: MiR-200a expression was analyzed in primary tumor specimens from 101 patients with HCC. The gene expression level of miR-200a was 0.59±0.08 in the tumor specimens and 1.40±0.41 in the corresponding non-tumor tissues (Fig. 1A). The index of miR-200a expression in the HCC cell lines MHCC-97H, HepG2, Huh-7 and SMMC-7721 was significantly lower than in the normal human hepatocyte cell line HL-7702 (Fig. 1B). We compared the low (51 cases) and high (50 cases) miR-200a expression groups (Table 1) and found that low expression of miR-200a strongly correlated with metastasis (P=0.011). The level of miR-200a expression was significantly lower in 74 cases of primary tumors with clinically confirmed metastasis compared with the 27 cases without metastasis (Fig. 1C).


MicroRNA-200a suppresses metastatic potential of side population cells in human hepatocellular carcinoma by decreasing ZEB2.

Yang X, Wang J, Qu S, Zhang H, Ruan B, Gao Y, Ma B, Wang X, Wu N, Li X, Dou K, Li H - Oncotarget (2015)

MiR-200a is down-regulated in HCC(A) The expression of miR-200a in HCC tissue specimens and in corresponding non-tumor tissues was measured by qRT-PCR. (B) The expression status of miR-200a in 4 human HCC cell lines and one normal human hepatocyte was measured by qRT-PCR. (C) The relative expression level of miR-200a in primary tumor samples with or without clinically confirmed metastasis was measured by qRT-PCR. (D) Kaplan-Meier analysis of the correlation between miR-200a expression and the overall survival of 101 patients with HCC. Patients in the low miR-200a expression group had a significantly shorter overall survival (P < 0.01); * P < 0.05, t test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4480725&req=5

Figure 1: MiR-200a is down-regulated in HCC(A) The expression of miR-200a in HCC tissue specimens and in corresponding non-tumor tissues was measured by qRT-PCR. (B) The expression status of miR-200a in 4 human HCC cell lines and one normal human hepatocyte was measured by qRT-PCR. (C) The relative expression level of miR-200a in primary tumor samples with or without clinically confirmed metastasis was measured by qRT-PCR. (D) Kaplan-Meier analysis of the correlation between miR-200a expression and the overall survival of 101 patients with HCC. Patients in the low miR-200a expression group had a significantly shorter overall survival (P < 0.01); * P < 0.05, t test.
Mentions: MiR-200a expression was analyzed in primary tumor specimens from 101 patients with HCC. The gene expression level of miR-200a was 0.59±0.08 in the tumor specimens and 1.40±0.41 in the corresponding non-tumor tissues (Fig. 1A). The index of miR-200a expression in the HCC cell lines MHCC-97H, HepG2, Huh-7 and SMMC-7721 was significantly lower than in the normal human hepatocyte cell line HL-7702 (Fig. 1B). We compared the low (51 cases) and high (50 cases) miR-200a expression groups (Table 1) and found that low expression of miR-200a strongly correlated with metastasis (P=0.011). The level of miR-200a expression was significantly lower in 74 cases of primary tumors with clinically confirmed metastasis compared with the 27 cases without metastasis (Fig. 1C).

Bottom Line: We found that miR-200a was downregulated in HCC/SP and this was associated metastasis.Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2.The associations between miR-200a, SP cells and ZEB2 were validated in HCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military Medical Uiversity, Xi'an, China.

ABSTRACT
Although microRNA-200a (miR-200a) is frequently downregulated in cancer, its role in side population (SP) has not been investigated. In this study, 101 pairs of primary hepatocellular carcinoma (HCC) tissues and matched normal control tissues were analyzed for miR-200a expression and its clinicopathological value was determined. We found that miR-200a was downregulated in HCC/SP and this was associated metastasis. MiR-200a suppressed metastasis of SP cells. Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2. The associations between miR-200a, SP cells and ZEB2 were validated in HCC. These findings reveal that miR-200a suppresses metastasis of SP cells by downregulating ZEB2.

No MeSH data available.


Related in: MedlinePlus