Limits...
Vasohibin-1 suppresses colon cancer.

Liu S, Han B, Zhang Q, Dou J, Wang F, Lin W, Sun Y, Peng G - Oncotarget (2015)

Bottom Line: Here we showed that stromal VASH1 levels were negatively correlated with tumor size, advanced clinical stage and distant metastases in colon cancer patients.Overexpression of VASH1 in colon cancer cells induced apoptosis and senescence, inhibiting cancer cell growth and colony formation in vitro and tumor growth in vivo.In addition, knockdown of VASH1 in cancer cells promoted cell growth, adhesion and migration in vitro, and enhanced tumorigenesis and metastasis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Jinan Central Hospital, Affiliated to Shandong University, Jinan, P. R. China.

ABSTRACT
Vasohibin-1 (VASH1) is an endogenous angiogenesis inhibitor.However, the clinical relevance of VASH1 in colon cancer and its regulations on cancer angiogenesis and cancer cell biological characteristics are still unknown. Here we showed that stromal VASH1 levels were negatively correlated with tumor size, advanced clinical stage and distant metastases in colon cancer patients. Overexpression of VASH1 in colon cancer cells induced apoptosis and senescence, inhibiting cancer cell growth and colony formation in vitro and tumor growth in vivo. In addition, knockdown of VASH1 in cancer cells promoted cell growth, adhesion and migration in vitro, and enhanced tumorigenesis and metastasis in vivo.

No MeSH data available.


Related in: MedlinePlus

Expression of VASH1 in cancer stroma of colon cancer patients(A) & (B) Significantly increased VASH1 expression density in endothelial cells of blood vessels was detected in colon cancer stroma, compared with that expressed in paracancerous normal tissues. Numbers of VASH1+ vessels in 75 colon cancer tissues and 59 paracancerous normal tissues were detected and summarized using the immunohistochemical staining. (C) & (D) Expression levels of angiogenic molecules CD34 and VEGF-A, as well as lymphoangiogenenic molecules D2-40 and VEGF-C in colon cancer tissues (n=75) and paracancerous normal tissues (n=59) were determined using the immunohistochemical staining. Expression level of each dot shown in (B) and (D) is the average numbers (VASH1, CD34 and D2-40) or scores (VEGF-A and VEGF-C) per high field (400 x) in each tissue sample. The mean number of each molecule in each group is shown as a horizontal line. Significance was determined by unpaired (cancer tissue vs paracancerous tissue) T test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4480723&req=5

Figure 1: Expression of VASH1 in cancer stroma of colon cancer patients(A) & (B) Significantly increased VASH1 expression density in endothelial cells of blood vessels was detected in colon cancer stroma, compared with that expressed in paracancerous normal tissues. Numbers of VASH1+ vessels in 75 colon cancer tissues and 59 paracancerous normal tissues were detected and summarized using the immunohistochemical staining. (C) & (D) Expression levels of angiogenic molecules CD34 and VEGF-A, as well as lymphoangiogenenic molecules D2-40 and VEGF-C in colon cancer tissues (n=75) and paracancerous normal tissues (n=59) were determined using the immunohistochemical staining. Expression level of each dot shown in (B) and (D) is the average numbers (VASH1, CD34 and D2-40) or scores (VEGF-A and VEGF-C) per high field (400 x) in each tissue sample. The mean number of each molecule in each group is shown as a horizontal line. Significance was determined by unpaired (cancer tissue vs paracancerous tissue) T test.

Mentions: Recent studies suggest that VASH1 is a novel angiogenic molecule that is critical for cancer angiogenesis and prognosis [19-25]. These novel findings prompted us to investigate the functional role of VASH1 in the pathogenesis of human colon cancer. We first performed immunohistochemical staining to detect VASH1 expression in 75 colon cancer tissues and 59 paracancerous normal tissues from cancer patients (Figure 1A & 1B). We found the prevalent expression of VASH1 in endothelial cells in both cancer stroma and paracancerous normal tissues (Figure 1A). However, in the paracancerous normal tissues, the numbers of VASH1+ vessels are very low (mean numbers of 3.1), whereas significantly increased numbers of VASH1 expression in vascular endothelial cells were detected in colon cancer stroma (mean numbers of 4.7) (Figure 1B). The result strongly suggested the activated angiogenesis in colon cancer patients. In addition, we investigated the expression levels of the other well-known angiogenic molecules CD34 and VEGF-A, as well as lymphoangiogenenic molecules D2-40 and VEGF-C in colon cancer tissues and paracancerous normal tissues (Figure 1C & 1D). CD34 expression was mainly localized in the cytoplasm and membrane of the blood endothelial cells, while D2-40 expression was observed in the cytoplasm and cellular membrane of lymph endothelial cells (Figure 1C). Furthermore, VEGF-A and VEGF-C were found expression in the cytoplasm both in cancer cells and in paracancerous normal tissues (Figure 1C). In addition, expression levels of CD34, D2-40, VEGF-A and VEGF-C in colon cancer tissues were significantly higher than those in paracancerous normal tissues (Figure 1D). Our results collectively suggest that both active angiogenesis and lymphoangiogenesis exist in colon cancer patients, and that VASH1 is prevalent in the cancer stroma of cancer tissues.


Vasohibin-1 suppresses colon cancer.

Liu S, Han B, Zhang Q, Dou J, Wang F, Lin W, Sun Y, Peng G - Oncotarget (2015)

Expression of VASH1 in cancer stroma of colon cancer patients(A) & (B) Significantly increased VASH1 expression density in endothelial cells of blood vessels was detected in colon cancer stroma, compared with that expressed in paracancerous normal tissues. Numbers of VASH1+ vessels in 75 colon cancer tissues and 59 paracancerous normal tissues were detected and summarized using the immunohistochemical staining. (C) & (D) Expression levels of angiogenic molecules CD34 and VEGF-A, as well as lymphoangiogenenic molecules D2-40 and VEGF-C in colon cancer tissues (n=75) and paracancerous normal tissues (n=59) were determined using the immunohistochemical staining. Expression level of each dot shown in (B) and (D) is the average numbers (VASH1, CD34 and D2-40) or scores (VEGF-A and VEGF-C) per high field (400 x) in each tissue sample. The mean number of each molecule in each group is shown as a horizontal line. Significance was determined by unpaired (cancer tissue vs paracancerous tissue) T test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4480723&req=5

Figure 1: Expression of VASH1 in cancer stroma of colon cancer patients(A) & (B) Significantly increased VASH1 expression density in endothelial cells of blood vessels was detected in colon cancer stroma, compared with that expressed in paracancerous normal tissues. Numbers of VASH1+ vessels in 75 colon cancer tissues and 59 paracancerous normal tissues were detected and summarized using the immunohistochemical staining. (C) & (D) Expression levels of angiogenic molecules CD34 and VEGF-A, as well as lymphoangiogenenic molecules D2-40 and VEGF-C in colon cancer tissues (n=75) and paracancerous normal tissues (n=59) were determined using the immunohistochemical staining. Expression level of each dot shown in (B) and (D) is the average numbers (VASH1, CD34 and D2-40) or scores (VEGF-A and VEGF-C) per high field (400 x) in each tissue sample. The mean number of each molecule in each group is shown as a horizontal line. Significance was determined by unpaired (cancer tissue vs paracancerous tissue) T test.
Mentions: Recent studies suggest that VASH1 is a novel angiogenic molecule that is critical for cancer angiogenesis and prognosis [19-25]. These novel findings prompted us to investigate the functional role of VASH1 in the pathogenesis of human colon cancer. We first performed immunohistochemical staining to detect VASH1 expression in 75 colon cancer tissues and 59 paracancerous normal tissues from cancer patients (Figure 1A & 1B). We found the prevalent expression of VASH1 in endothelial cells in both cancer stroma and paracancerous normal tissues (Figure 1A). However, in the paracancerous normal tissues, the numbers of VASH1+ vessels are very low (mean numbers of 3.1), whereas significantly increased numbers of VASH1 expression in vascular endothelial cells were detected in colon cancer stroma (mean numbers of 4.7) (Figure 1B). The result strongly suggested the activated angiogenesis in colon cancer patients. In addition, we investigated the expression levels of the other well-known angiogenic molecules CD34 and VEGF-A, as well as lymphoangiogenenic molecules D2-40 and VEGF-C in colon cancer tissues and paracancerous normal tissues (Figure 1C & 1D). CD34 expression was mainly localized in the cytoplasm and membrane of the blood endothelial cells, while D2-40 expression was observed in the cytoplasm and cellular membrane of lymph endothelial cells (Figure 1C). Furthermore, VEGF-A and VEGF-C were found expression in the cytoplasm both in cancer cells and in paracancerous normal tissues (Figure 1C). In addition, expression levels of CD34, D2-40, VEGF-A and VEGF-C in colon cancer tissues were significantly higher than those in paracancerous normal tissues (Figure 1D). Our results collectively suggest that both active angiogenesis and lymphoangiogenesis exist in colon cancer patients, and that VASH1 is prevalent in the cancer stroma of cancer tissues.

Bottom Line: Here we showed that stromal VASH1 levels were negatively correlated with tumor size, advanced clinical stage and distant metastases in colon cancer patients.Overexpression of VASH1 in colon cancer cells induced apoptosis and senescence, inhibiting cancer cell growth and colony formation in vitro and tumor growth in vivo.In addition, knockdown of VASH1 in cancer cells promoted cell growth, adhesion and migration in vitro, and enhanced tumorigenesis and metastasis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Jinan Central Hospital, Affiliated to Shandong University, Jinan, P. R. China.

ABSTRACT
Vasohibin-1 (VASH1) is an endogenous angiogenesis inhibitor.However, the clinical relevance of VASH1 in colon cancer and its regulations on cancer angiogenesis and cancer cell biological characteristics are still unknown. Here we showed that stromal VASH1 levels were negatively correlated with tumor size, advanced clinical stage and distant metastases in colon cancer patients. Overexpression of VASH1 in colon cancer cells induced apoptosis and senescence, inhibiting cancer cell growth and colony formation in vitro and tumor growth in vivo. In addition, knockdown of VASH1 in cancer cells promoted cell growth, adhesion and migration in vitro, and enhanced tumorigenesis and metastasis in vivo.

No MeSH data available.


Related in: MedlinePlus