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Adipose-derived stem cells promote tumor initiation and accelerate tumor growth by interleukin-6 production.

Wei HJ, Zeng R, Lu JH, Lai WF, Chen WH, Liu HY, Chang YT, Deng WP - Oncotarget (2015)

Bottom Line: Here, we show that ADSCs enhance sphere formation and in vivo tumor initiation of breast and colon cancer cells.ADSCs also accelerated tumor growth.We suggest that ADSCs may enhance tumor initiation and promotion.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Materials and Engineering, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.

ABSTRACT
Adipose-derived stem cells (ADSCs) are multipotent cells that have attracted much recent attention. Here, we show that ADSCs enhance sphere formation and in vivo tumor initiation of breast and colon cancer cells. In co-culture, ADSCs induced several stem cell markers in cancer cells. ADSCs also accelerated tumor growth. Interaction of ADSCs and cancer cells stimulated secretion of interlukin-6 in ADSCs, which in turn acted in a paracrine manner on cancer cells to enhance their malignant properties. Interleukin-6 regulated stem cell-related genes and activated JAK2/STAT3 in cancer cells. We suggest that ADSCs may enhance tumor initiation and promotion.

No MeSH data available.


Related in: MedlinePlus

Activation of IL-6-dependent pathway in breast and colon cancer cells upon co-culture with ADSCs(A) Protein level of phosphorylated JAK2 and STAT3 in 4T1 and CT26 cells upon co-culture with ADSCs and treated with IL-6 neutralizing antibody (Anti-IL-6), with actin as loading control. (B) A schematic showing that IL-6 mediates the tumor-promoting effects of ADSCs in tumor development.
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Figure 7: Activation of IL-6-dependent pathway in breast and colon cancer cells upon co-culture with ADSCs(A) Protein level of phosphorylated JAK2 and STAT3 in 4T1 and CT26 cells upon co-culture with ADSCs and treated with IL-6 neutralizing antibody (Anti-IL-6), with actin as loading control. (B) A schematic showing that IL-6 mediates the tumor-promoting effects of ADSCs in tumor development.

Mentions: Above results suggest that ADSC-derived IL-6 played a critical role in activating malignant characteristics of cancer cells in cancer/ADSC interaction. To identify what downstream signals in cancer cells respond to IL-6, we looked at JAK2/STAT3 pathway. JAK2/STAT3 have been reported to be the predominant pathway activated by IL-6 [27]. As shown in Figure 7A, the protein levels of phosphorylated JAK2 and STAT3 in cancer cells were increased upon co-culture with ADSCs. Nonetheless, ADSCs-induced phosphorylations of JAK2 and STAT3 were inhibited by treatment with IL-6–neutralized antibodies. Together these results demonstrate that upon co-culture with cancer cells ADSCs can activate the JAK2/STAT3 pathways in both breast and colon cancer cells via induced IL-6 expression.


Adipose-derived stem cells promote tumor initiation and accelerate tumor growth by interleukin-6 production.

Wei HJ, Zeng R, Lu JH, Lai WF, Chen WH, Liu HY, Chang YT, Deng WP - Oncotarget (2015)

Activation of IL-6-dependent pathway in breast and colon cancer cells upon co-culture with ADSCs(A) Protein level of phosphorylated JAK2 and STAT3 in 4T1 and CT26 cells upon co-culture with ADSCs and treated with IL-6 neutralizing antibody (Anti-IL-6), with actin as loading control. (B) A schematic showing that IL-6 mediates the tumor-promoting effects of ADSCs in tumor development.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4480711&req=5

Figure 7: Activation of IL-6-dependent pathway in breast and colon cancer cells upon co-culture with ADSCs(A) Protein level of phosphorylated JAK2 and STAT3 in 4T1 and CT26 cells upon co-culture with ADSCs and treated with IL-6 neutralizing antibody (Anti-IL-6), with actin as loading control. (B) A schematic showing that IL-6 mediates the tumor-promoting effects of ADSCs in tumor development.
Mentions: Above results suggest that ADSC-derived IL-6 played a critical role in activating malignant characteristics of cancer cells in cancer/ADSC interaction. To identify what downstream signals in cancer cells respond to IL-6, we looked at JAK2/STAT3 pathway. JAK2/STAT3 have been reported to be the predominant pathway activated by IL-6 [27]. As shown in Figure 7A, the protein levels of phosphorylated JAK2 and STAT3 in cancer cells were increased upon co-culture with ADSCs. Nonetheless, ADSCs-induced phosphorylations of JAK2 and STAT3 were inhibited by treatment with IL-6–neutralized antibodies. Together these results demonstrate that upon co-culture with cancer cells ADSCs can activate the JAK2/STAT3 pathways in both breast and colon cancer cells via induced IL-6 expression.

Bottom Line: Here, we show that ADSCs enhance sphere formation and in vivo tumor initiation of breast and colon cancer cells.ADSCs also accelerated tumor growth.We suggest that ADSCs may enhance tumor initiation and promotion.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biomedical Materials and Engineering, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.

ABSTRACT
Adipose-derived stem cells (ADSCs) are multipotent cells that have attracted much recent attention. Here, we show that ADSCs enhance sphere formation and in vivo tumor initiation of breast and colon cancer cells. In co-culture, ADSCs induced several stem cell markers in cancer cells. ADSCs also accelerated tumor growth. Interaction of ADSCs and cancer cells stimulated secretion of interlukin-6 in ADSCs, which in turn acted in a paracrine manner on cancer cells to enhance their malignant properties. Interleukin-6 regulated stem cell-related genes and activated JAK2/STAT3 in cancer cells. We suggest that ADSCs may enhance tumor initiation and promotion.

No MeSH data available.


Related in: MedlinePlus