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Programmable wireless light-emitting diode stimulator for chronic stimulation of optogenetic molecules in freely moving mice.

Hashimoto M, Hata A, Miyata T, Hirase H - Neurophotonics (2014)

Bottom Line: Individual LED photopulse patterns are assigned to different codes of the IR signals (up to 256 codes).The photopulse patterns can be programmed in the on-board microcontroller by specifying the parameters of duration ([Formula: see text]), frequency ([Formula: see text]), and pulse width ([Formula: see text]).IR transmitter and LED stimulator will be particularly useful in experiments where free movement or patterned concurrent stimulation is desired, such as testing social communication of rodents.

View Article: PubMed Central - PubMed

Affiliation: Nagoya University Graduate School of Medicine , Department of Anatomy and Cell Biology, 65 Tsurumai-cho, Showa-ku, Nagoya-shi, Aichi 466-8550, Japan.

ABSTRACT
We produced a miniaturized, multicode, multiband, and programmable light-emitting diode (LED) stimulator for wireless control of optogenetic experiments. The LED stimulator is capable of driving three independent LEDs upon reception of an infrared (IR) signal generated by a custom-made IR transmitter. Individual LED photopulse patterns are assigned to different codes of the IR signals (up to 256 codes). The photopulse patterns can be programmed in the on-board microcontroller by specifying the parameters of duration ([Formula: see text]), frequency ([Formula: see text]), and pulse width ([Formula: see text]). The IR signals were modulated at multiple carrier frequencies to establish multiband IR transmission. Using these devices, we could remotely control the moving direction of a Thy1-ChR2-YFP transgenic mouse by transcranially illuminating the corresponding hemisphere of the primary motor cortex. IR transmitter and LED stimulator will be particularly useful in experiments where free movement or patterned concurrent stimulation is desired, such as testing social communication of rodents.

No MeSH data available.


Related in: MedlinePlus

Use of multiband LED stimulators in the same field. The IR transmitters and LED stimulators were adapted to 30-kHz (labeled 30 kHz) and 56-kHz IR photopulses (labeled 56 kHz). A shell-type LED placed on the IR stimulator flashed simultaneously with a TTL trigger signal input into the IR stimulator. (QuickTime, 8.7 MB) [URL: http://dx.doi.org/10.1117/1.NPh.1.1.011002.3].Click here for additional data file.
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f10: Use of multiband LED stimulators in the same field. The IR transmitters and LED stimulators were adapted to 30-kHz (labeled 30 kHz) and 56-kHz IR photopulses (labeled 56 kHz). A shell-type LED placed on the IR stimulator flashed simultaneously with a TTL trigger signal input into the IR stimulator. (QuickTime, 8.7 MB) [URL: http://dx.doi.org/10.1117/1.NPh.1.1.011002.3].Click here for additional data file.

Mentions: We used 8-bit binary codes to discriminate among channels (e.g., Ch.3, 00001111) in the wireless LED-stimulating system (Fig. 2), allowing up to 256 codes. For multidevice applications, individual LED stimulators can be programmed to recognize unique 8-bit binary codes. For instance, we modified one LED stimulator to recognize the 8-bit binary codes of Ch.5 to Ch.8 (Ch.5, 00011101, LED5; Ch.6, 00011110, LED6; Ch.7, 00011111, LED7; Ch.8, 00000100, LED5 and LED6; LED-stimulator No. 2, Video 2) and used it with another LED stimulator that recognized Ch.1 to Ch.4 (Ch.1, 00001101, LED1; Ch.2, 00001110, LED2; Ch.3, 00001111, LED3; Ch.4, 00011100, LED1 and LED2; LED stimulator No. 1, Video 2) in the same field. Each channel of the two LED stimulators could individually be controlled by the IR photopulses of Ch.1 to Ch.8 without crosstalk. Furthermore, to establish IR transmission at multiple frequency bands, we adapted the IR transmitter and LED stimulator for carrier frequencies of 30, 38, and 56 kHz. The modified LED stimulators discriminated between 30- and 56-kHz IR photopulses, but 38-kHz transmission was received by both 30- and 56-kHz devices. Therefore, carrier frequencies of 30 and 56 kHz can be used for multiband IR transmission (Video 3). Consequently, we could use multiple LED stimulators for photostimulation of animals in the same experimental arena at the same time.


Programmable wireless light-emitting diode stimulator for chronic stimulation of optogenetic molecules in freely moving mice.

Hashimoto M, Hata A, Miyata T, Hirase H - Neurophotonics (2014)

Use of multiband LED stimulators in the same field. The IR transmitters and LED stimulators were adapted to 30-kHz (labeled 30 kHz) and 56-kHz IR photopulses (labeled 56 kHz). A shell-type LED placed on the IR stimulator flashed simultaneously with a TTL trigger signal input into the IR stimulator. (QuickTime, 8.7 MB) [URL: http://dx.doi.org/10.1117/1.NPh.1.1.011002.3].Click here for additional data file.
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Related In: Results  -  Collection

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f10: Use of multiband LED stimulators in the same field. The IR transmitters and LED stimulators were adapted to 30-kHz (labeled 30 kHz) and 56-kHz IR photopulses (labeled 56 kHz). A shell-type LED placed on the IR stimulator flashed simultaneously with a TTL trigger signal input into the IR stimulator. (QuickTime, 8.7 MB) [URL: http://dx.doi.org/10.1117/1.NPh.1.1.011002.3].Click here for additional data file.
Mentions: We used 8-bit binary codes to discriminate among channels (e.g., Ch.3, 00001111) in the wireless LED-stimulating system (Fig. 2), allowing up to 256 codes. For multidevice applications, individual LED stimulators can be programmed to recognize unique 8-bit binary codes. For instance, we modified one LED stimulator to recognize the 8-bit binary codes of Ch.5 to Ch.8 (Ch.5, 00011101, LED5; Ch.6, 00011110, LED6; Ch.7, 00011111, LED7; Ch.8, 00000100, LED5 and LED6; LED-stimulator No. 2, Video 2) and used it with another LED stimulator that recognized Ch.1 to Ch.4 (Ch.1, 00001101, LED1; Ch.2, 00001110, LED2; Ch.3, 00001111, LED3; Ch.4, 00011100, LED1 and LED2; LED stimulator No. 1, Video 2) in the same field. Each channel of the two LED stimulators could individually be controlled by the IR photopulses of Ch.1 to Ch.8 without crosstalk. Furthermore, to establish IR transmission at multiple frequency bands, we adapted the IR transmitter and LED stimulator for carrier frequencies of 30, 38, and 56 kHz. The modified LED stimulators discriminated between 30- and 56-kHz IR photopulses, but 38-kHz transmission was received by both 30- and 56-kHz devices. Therefore, carrier frequencies of 30 and 56 kHz can be used for multiband IR transmission (Video 3). Consequently, we could use multiple LED stimulators for photostimulation of animals in the same experimental arena at the same time.

Bottom Line: Individual LED photopulse patterns are assigned to different codes of the IR signals (up to 256 codes).The photopulse patterns can be programmed in the on-board microcontroller by specifying the parameters of duration ([Formula: see text]), frequency ([Formula: see text]), and pulse width ([Formula: see text]).IR transmitter and LED stimulator will be particularly useful in experiments where free movement or patterned concurrent stimulation is desired, such as testing social communication of rodents.

View Article: PubMed Central - PubMed

Affiliation: Nagoya University Graduate School of Medicine , Department of Anatomy and Cell Biology, 65 Tsurumai-cho, Showa-ku, Nagoya-shi, Aichi 466-8550, Japan.

ABSTRACT
We produced a miniaturized, multicode, multiband, and programmable light-emitting diode (LED) stimulator for wireless control of optogenetic experiments. The LED stimulator is capable of driving three independent LEDs upon reception of an infrared (IR) signal generated by a custom-made IR transmitter. Individual LED photopulse patterns are assigned to different codes of the IR signals (up to 256 codes). The photopulse patterns can be programmed in the on-board microcontroller by specifying the parameters of duration ([Formula: see text]), frequency ([Formula: see text]), and pulse width ([Formula: see text]). The IR signals were modulated at multiple carrier frequencies to establish multiband IR transmission. Using these devices, we could remotely control the moving direction of a Thy1-ChR2-YFP transgenic mouse by transcranially illuminating the corresponding hemisphere of the primary motor cortex. IR transmitter and LED stimulator will be particularly useful in experiments where free movement or patterned concurrent stimulation is desired, such as testing social communication of rodents.

No MeSH data available.


Related in: MedlinePlus