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Dietary Silicon Deficiency Does Not Exacerbate Diet-Induced Fatty Lesions in Female ApoE Knockout Mice.

Jugdaohsingh R, Kessler K, Messner B, Stoiber M, Pedro LD, Schima H, Laufer G, Powell JJ, Bernhard D - J. Nutr. (2015)

Bottom Line: The serum silicon concentration in the -Si group was significantly lower than in the +Si-feed (by up to 78%; P < 0.003) and the +Si-water (by up to 84%; P < 0.006) groups.The aorta silicon concentration was also lower in the -Si group than in the +Si-feed group (by 65%; P = 0.025), but not compared with the +Si-water group.These findings suggest that dietary silicon has no effect on atherosclerosis development and vascular health in the apoE mouse model of diet-induced atherosclerosis, contrary to the reported findings in the cholesterol-fed rabbit model.

View Article: PubMed Central - PubMed

Affiliation: Medical Research Council (MRC) Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, United Kingdom; ravin.jugdaohsingh@mrc-hnr.cam.ac.uk.

ABSTRACT

Background: Dietary silicon has been positively linked with vascular health and protection against atherosclerotic plaque formation, but the mechanism of action is unclear.

Objectives: We investigated the effect of dietary silicon on 1) serum and aorta silicon concentrations, 2) the development of aortic lesions and serum lipid concentrations, and 3) the structural and biomechanic properties of the aorta.

Methods: Two studies, of the same design, were conducted to address the above objectives. Female mice, lacking the apolipoprotein E (apoE) gene, and therefore susceptible to atherosclerosis, were separated into 3 groups of 10-15 mice, each exposed to a high-fat diet (21% wt milk fat and 1.5% wt cholesterol) but with differing concentrations of dietary silicon, namely: silicon-deprived (-Si; <3-μg silicon/g feed), silicon-replete in feed (+Si-feed; 100-μg silicon/g feed), and silicon-replete in drinking water (+Si-water; 115-μg silicon/mL) for 15-19 wk. Silicon supplementation was in the form of sodium metasilicate (feed) or monomethylsilanetriol (drinking water).

Results: The serum silicon concentration in the -Si group was significantly lower than in the +Si-feed (by up to 78%; P < 0.003) and the +Si-water (by up to 84%; P < 0.006) groups. The aorta silicon concentration was also lower in the -Si group than in the +Si-feed group (by 65%; P = 0.025), but not compared with the +Si-water group. There were no differences in serum and aorta silicon concentrations between the silicon-replete groups. Body weights, tissue wet weights at necropsy, and structural, biomechanic, and morphologic properties of the aorta were not affected by dietary silicon; nor were the development of fatty lesions and serum lipid concentrations.

Conclusions: These findings suggest that dietary silicon has no effect on atherosclerosis development and vascular health in the apoE mouse model of diet-induced atherosclerosis, contrary to the reported findings in the cholesterol-fed rabbit model.

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Related in: MedlinePlus

Total silicon concentration of the aorta of female apoE knockout mice in Study 1 fed a diet high in butter fat and depleted in silicon, compared with mice fed the 2 silicon-replete, high-fat diets: silicon was replete in the feed or the drinking water. Values (n = 6–10/group) are shown as box-plots where the horizontal lines indicate the 5th, 25th, 50th (i.e., median), 75th, and 95th percentiles, the open squares show the means, and the crosses show the minimum and maximum values. Mice were deprived of food overnight before collection of aorta. Within the graph, labeled groups without a common letter differ (P < 0.05; independent samples Kruskal-Wallis test with pairwise comparison). Si, silicon; −Si, silicon-deprived; +Si-feed, silicon-replete in feed; +Si-water, silicon-replete in drinking water.
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fig3: Total silicon concentration of the aorta of female apoE knockout mice in Study 1 fed a diet high in butter fat and depleted in silicon, compared with mice fed the 2 silicon-replete, high-fat diets: silicon was replete in the feed or the drinking water. Values (n = 6–10/group) are shown as box-plots where the horizontal lines indicate the 5th, 25th, 50th (i.e., median), 75th, and 95th percentiles, the open squares show the means, and the crosses show the minimum and maximum values. Mice were deprived of food overnight before collection of aorta. Within the graph, labeled groups without a common letter differ (P < 0.05; independent samples Kruskal-Wallis test with pairwise comparison). Si, silicon; −Si, silicon-deprived; +Si-feed, silicon-replete in feed; +Si-water, silicon-replete in drinking water.

Mentions: Silicon concentration of the whole aorta collected in Study 1 was 65% lower in the −Si group than the +Si-feed group (P = 0.025), but was not different compared to the +Si-water group (Figure 3). Again, there was no difference in (aortic) silicon concentration between the +Si groups.


Dietary Silicon Deficiency Does Not Exacerbate Diet-Induced Fatty Lesions in Female ApoE Knockout Mice.

Jugdaohsingh R, Kessler K, Messner B, Stoiber M, Pedro LD, Schima H, Laufer G, Powell JJ, Bernhard D - J. Nutr. (2015)

Total silicon concentration of the aorta of female apoE knockout mice in Study 1 fed a diet high in butter fat and depleted in silicon, compared with mice fed the 2 silicon-replete, high-fat diets: silicon was replete in the feed or the drinking water. Values (n = 6–10/group) are shown as box-plots where the horizontal lines indicate the 5th, 25th, 50th (i.e., median), 75th, and 95th percentiles, the open squares show the means, and the crosses show the minimum and maximum values. Mice were deprived of food overnight before collection of aorta. Within the graph, labeled groups without a common letter differ (P < 0.05; independent samples Kruskal-Wallis test with pairwise comparison). Si, silicon; −Si, silicon-deprived; +Si-feed, silicon-replete in feed; +Si-water, silicon-replete in drinking water.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4478943&req=5

fig3: Total silicon concentration of the aorta of female apoE knockout mice in Study 1 fed a diet high in butter fat and depleted in silicon, compared with mice fed the 2 silicon-replete, high-fat diets: silicon was replete in the feed or the drinking water. Values (n = 6–10/group) are shown as box-plots where the horizontal lines indicate the 5th, 25th, 50th (i.e., median), 75th, and 95th percentiles, the open squares show the means, and the crosses show the minimum and maximum values. Mice were deprived of food overnight before collection of aorta. Within the graph, labeled groups without a common letter differ (P < 0.05; independent samples Kruskal-Wallis test with pairwise comparison). Si, silicon; −Si, silicon-deprived; +Si-feed, silicon-replete in feed; +Si-water, silicon-replete in drinking water.
Mentions: Silicon concentration of the whole aorta collected in Study 1 was 65% lower in the −Si group than the +Si-feed group (P = 0.025), but was not different compared to the +Si-water group (Figure 3). Again, there was no difference in (aortic) silicon concentration between the +Si groups.

Bottom Line: The serum silicon concentration in the -Si group was significantly lower than in the +Si-feed (by up to 78%; P < 0.003) and the +Si-water (by up to 84%; P < 0.006) groups.The aorta silicon concentration was also lower in the -Si group than in the +Si-feed group (by 65%; P = 0.025), but not compared with the +Si-water group.These findings suggest that dietary silicon has no effect on atherosclerosis development and vascular health in the apoE mouse model of diet-induced atherosclerosis, contrary to the reported findings in the cholesterol-fed rabbit model.

View Article: PubMed Central - PubMed

Affiliation: Medical Research Council (MRC) Human Nutrition Research, Elsie Widdowson Laboratory, Cambridge, United Kingdom; ravin.jugdaohsingh@mrc-hnr.cam.ac.uk.

ABSTRACT

Background: Dietary silicon has been positively linked with vascular health and protection against atherosclerotic plaque formation, but the mechanism of action is unclear.

Objectives: We investigated the effect of dietary silicon on 1) serum and aorta silicon concentrations, 2) the development of aortic lesions and serum lipid concentrations, and 3) the structural and biomechanic properties of the aorta.

Methods: Two studies, of the same design, were conducted to address the above objectives. Female mice, lacking the apolipoprotein E (apoE) gene, and therefore susceptible to atherosclerosis, were separated into 3 groups of 10-15 mice, each exposed to a high-fat diet (21% wt milk fat and 1.5% wt cholesterol) but with differing concentrations of dietary silicon, namely: silicon-deprived (-Si; <3-μg silicon/g feed), silicon-replete in feed (+Si-feed; 100-μg silicon/g feed), and silicon-replete in drinking water (+Si-water; 115-μg silicon/mL) for 15-19 wk. Silicon supplementation was in the form of sodium metasilicate (feed) or monomethylsilanetriol (drinking water).

Results: The serum silicon concentration in the -Si group was significantly lower than in the +Si-feed (by up to 78%; P < 0.003) and the +Si-water (by up to 84%; P < 0.006) groups. The aorta silicon concentration was also lower in the -Si group than in the +Si-feed group (by 65%; P = 0.025), but not compared with the +Si-water group. There were no differences in serum and aorta silicon concentrations between the silicon-replete groups. Body weights, tissue wet weights at necropsy, and structural, biomechanic, and morphologic properties of the aorta were not affected by dietary silicon; nor were the development of fatty lesions and serum lipid concentrations.

Conclusions: These findings suggest that dietary silicon has no effect on atherosclerosis development and vascular health in the apoE mouse model of diet-induced atherosclerosis, contrary to the reported findings in the cholesterol-fed rabbit model.

Show MeSH
Related in: MedlinePlus