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Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans, animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis.

Ido N, Iwabuchi K, Sato'o Y, Sato Y, Sugawara M, Yaegashi G, Konno M, Akiba M, Tanaka K, Omoe K, Uchida I - J. Vet. Med. Sci. (2015)

Bottom Line: Fifty-one Salmonella enterica serovar 4,[5],12:i:- (S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3 river water strains) which are assumed to be monophasic variants of S.These results suggested that S. 4,[5],12:i:- originated from multiple S.Typhimurium ancestors.

View Article: PubMed Central - PubMed

Affiliation: Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa, Japan.

ABSTRACT
Fifty-one Salmonella enterica serovar 4,[5],12:i:- (S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3 river water strains) which are assumed to be monophasic variants of S. Typhimurium were analyzed using pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA) in order to investigate their genetic diversities and relationships. PFGE, MLVA and combination of them identified 28, 27 and 34 profiles (Simpson's diversity indices [DI]=0.94, 0.96 and 0.97), respectively. No correlations were detected between MLVA clustering and PFGE clustering or phage typing. These results suggested that S. 4,[5],12:i:- originated from multiple S. Typhimurium ancestors. Two cattle and one pig isolates showing identical phage types as well as PFGE and MLVA profiles to human isolates S. 4,[5],12:i:- suggested the existence of the links between human infections and animal reservoirs.

No MeSH data available.


Related in: MedlinePlus

Results of genotyping of S. 4,[5],12:i:- isolated from humans andanimals in Japan. A dendrogram prepared from the PFGE profiles afterBlnI digestion is placed on the left with their PFGE profiles.Detailed assignment of the results of PFGE and MLVA typing, epidemiological informationand presence of fljAB operon [10] of the isolates are indicated in the table placed on the right. MLVAprofiles are composed of five numbers indicating the repeat unit for each locus, in thefollowing order: STTR9-STTR5-STTR6-STTR10pl-STTR3 [13]. aThe alphabets indicate the sources of isolates; C, cattle; H,human; S, swine; K, chickens; B, birds; M, pork; R, river water. bThe datawere cited from the previous study [10].
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fig_001: Results of genotyping of S. 4,[5],12:i:- isolated from humans andanimals in Japan. A dendrogram prepared from the PFGE profiles afterBlnI digestion is placed on the left with their PFGE profiles.Detailed assignment of the results of PFGE and MLVA typing, epidemiological informationand presence of fljAB operon [10] of the isolates are indicated in the table placed on the right. MLVAprofiles are composed of five numbers indicating the repeat unit for each locus, in thefollowing order: STTR9-STTR5-STTR6-STTR10pl-STTR3 [13]. aThe alphabets indicate the sources of isolates; C, cattle; H,human; S, swine; K, chickens; B, birds; M, pork; R, river water. bThe datawere cited from the previous study [10].

Mentions: Figure 1Fig. 1.


Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans, animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis.

Ido N, Iwabuchi K, Sato'o Y, Sato Y, Sugawara M, Yaegashi G, Konno M, Akiba M, Tanaka K, Omoe K, Uchida I - J. Vet. Med. Sci. (2015)

Results of genotyping of S. 4,[5],12:i:- isolated from humans andanimals in Japan. A dendrogram prepared from the PFGE profiles afterBlnI digestion is placed on the left with their PFGE profiles.Detailed assignment of the results of PFGE and MLVA typing, epidemiological informationand presence of fljAB operon [10] of the isolates are indicated in the table placed on the right. MLVAprofiles are composed of five numbers indicating the repeat unit for each locus, in thefollowing order: STTR9-STTR5-STTR6-STTR10pl-STTR3 [13]. aThe alphabets indicate the sources of isolates; C, cattle; H,human; S, swine; K, chickens; B, birds; M, pork; R, river water. bThe datawere cited from the previous study [10].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4478744&req=5

fig_001: Results of genotyping of S. 4,[5],12:i:- isolated from humans andanimals in Japan. A dendrogram prepared from the PFGE profiles afterBlnI digestion is placed on the left with their PFGE profiles.Detailed assignment of the results of PFGE and MLVA typing, epidemiological informationand presence of fljAB operon [10] of the isolates are indicated in the table placed on the right. MLVAprofiles are composed of five numbers indicating the repeat unit for each locus, in thefollowing order: STTR9-STTR5-STTR6-STTR10pl-STTR3 [13]. aThe alphabets indicate the sources of isolates; C, cattle; H,human; S, swine; K, chickens; B, birds; M, pork; R, river water. bThe datawere cited from the previous study [10].
Mentions: Figure 1Fig. 1.

Bottom Line: Fifty-one Salmonella enterica serovar 4,[5],12:i:- (S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3 river water strains) which are assumed to be monophasic variants of S.These results suggested that S. 4,[5],12:i:- originated from multiple S.Typhimurium ancestors.

View Article: PubMed Central - PubMed

Affiliation: Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa, Japan.

ABSTRACT
Fifty-one Salmonella enterica serovar 4,[5],12:i:- (S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3 river water strains) which are assumed to be monophasic variants of S. Typhimurium were analyzed using pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA) in order to investigate their genetic diversities and relationships. PFGE, MLVA and combination of them identified 28, 27 and 34 profiles (Simpson's diversity indices [DI]=0.94, 0.96 and 0.97), respectively. No correlations were detected between MLVA clustering and PFGE clustering or phage typing. These results suggested that S. 4,[5],12:i:- originated from multiple S. Typhimurium ancestors. Two cattle and one pig isolates showing identical phage types as well as PFGE and MLVA profiles to human isolates S. 4,[5],12:i:- suggested the existence of the links between human infections and animal reservoirs.

No MeSH data available.


Related in: MedlinePlus