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Laser induced injury caused hyperglycemia-like effect in Drosophila larva: a possible insect model for posttraumatic diabetes.

Okabe F, Nakagiri Y, Yamada T, Kose H - J. Vet. Med. Sci. (2015)

Bottom Line: Diabetic patients need particular care in case of infection, digestive disorder or external injury, because external stress often exasperates the glucose metabolism, which is known as "sick day management".In addition, severe trauma can be a cause of hyperglycemia with insulin resistance.Here, we ablated body wall muscles in Drosophila larvae by laser beam and found that the level of trehalose, the principal sugar circulating in the hemolymph or in the tissues of most insects, was increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Division of Natural Sciences, International Christian University, Mitaka, Tokyo 181-8585, Japan.

ABSTRACT
Diabetic patients need particular care in case of infection, digestive disorder or external injury, because external stress often exasperates the glucose metabolism, which is known as "sick day management". In addition, severe trauma can be a cause of hyperglycemia with insulin resistance. In spite of critical component of the treatment, the precise mechanisms of how trauma develops posttraumatic diabetes remain unknown. Here, we ablated body wall muscles in Drosophila larvae by laser beam and found that the level of trehalose, the principal sugar circulating in the hemolymph or in the tissues of most insects, was increased. The model may provide a helpful tool to understand the relationship between trauma and sugar metabolism.

No MeSH data available.


Related in: MedlinePlus

Laser ablation of body wall results in increased trehalose levels. 10 muscle ablated(MA): Ten muscle fibers were lesioned using GFP signal as a guide to targetventrolateral body wall muscle 12 (VLM12). VLM12 is GFP labeled in5053A-Gal4>UAS-2XEGFP (A, allows). Ablation was confirmed by the lack of GFP signal(arrows) (B). 30MA: Thirty muscle fibers, mostly in 1 or 2 abdominal segments, wereablated in EP6310>UAS-2XEGFP, which expresses GFP in all body muscles. Area wheremuscles were ablated is shown in box. (C). After the ablation, no apparent damages werefound in the surrounding tissues: trachea (arrowheads), fat body (*), gut (#) (D). Bars:200 µm.
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fig_001: Laser ablation of body wall results in increased trehalose levels. 10 muscle ablated(MA): Ten muscle fibers were lesioned using GFP signal as a guide to targetventrolateral body wall muscle 12 (VLM12). VLM12 is GFP labeled in5053A-Gal4>UAS-2XEGFP (A, allows). Ablation was confirmed by the lack of GFP signal(arrows) (B). 30MA: Thirty muscle fibers, mostly in 1 or 2 abdominal segments, wereablated in EP6310>UAS-2XEGFP, which expresses GFP in all body muscles. Area wheremuscles were ablated is shown in box. (C). After the ablation, no apparent damages werefound in the surrounding tissues: trachea (arrowheads), fat body (*), gut (#) (D). Bars:200 µm.

Mentions: Drosophila larvae body wall muscles consisted of about 580 muscle fibers,each of which can be distinguished from its neighbors by its size, shape and attachment sites[1]. In order to target specific fibers, 5053A-Ga4strain was chosen to mark, among 30 muscle fibers in each of hemisegments of A2–A7, oneparticular fibers called ventrolateral body wall muscle 12 (VLM12) (Fig. 1AFig. 1.


Laser induced injury caused hyperglycemia-like effect in Drosophila larva: a possible insect model for posttraumatic diabetes.

Okabe F, Nakagiri Y, Yamada T, Kose H - J. Vet. Med. Sci. (2015)

Laser ablation of body wall results in increased trehalose levels. 10 muscle ablated(MA): Ten muscle fibers were lesioned using GFP signal as a guide to targetventrolateral body wall muscle 12 (VLM12). VLM12 is GFP labeled in5053A-Gal4>UAS-2XEGFP (A, allows). Ablation was confirmed by the lack of GFP signal(arrows) (B). 30MA: Thirty muscle fibers, mostly in 1 or 2 abdominal segments, wereablated in EP6310>UAS-2XEGFP, which expresses GFP in all body muscles. Area wheremuscles were ablated is shown in box. (C). After the ablation, no apparent damages werefound in the surrounding tissues: trachea (arrowheads), fat body (*), gut (#) (D). Bars:200 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4478742&req=5

fig_001: Laser ablation of body wall results in increased trehalose levels. 10 muscle ablated(MA): Ten muscle fibers were lesioned using GFP signal as a guide to targetventrolateral body wall muscle 12 (VLM12). VLM12 is GFP labeled in5053A-Gal4>UAS-2XEGFP (A, allows). Ablation was confirmed by the lack of GFP signal(arrows) (B). 30MA: Thirty muscle fibers, mostly in 1 or 2 abdominal segments, wereablated in EP6310>UAS-2XEGFP, which expresses GFP in all body muscles. Area wheremuscles were ablated is shown in box. (C). After the ablation, no apparent damages werefound in the surrounding tissues: trachea (arrowheads), fat body (*), gut (#) (D). Bars:200 µm.
Mentions: Drosophila larvae body wall muscles consisted of about 580 muscle fibers,each of which can be distinguished from its neighbors by its size, shape and attachment sites[1]. In order to target specific fibers, 5053A-Ga4strain was chosen to mark, among 30 muscle fibers in each of hemisegments of A2–A7, oneparticular fibers called ventrolateral body wall muscle 12 (VLM12) (Fig. 1AFig. 1.

Bottom Line: Diabetic patients need particular care in case of infection, digestive disorder or external injury, because external stress often exasperates the glucose metabolism, which is known as "sick day management".In addition, severe trauma can be a cause of hyperglycemia with insulin resistance.Here, we ablated body wall muscles in Drosophila larvae by laser beam and found that the level of trehalose, the principal sugar circulating in the hemolymph or in the tissues of most insects, was increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Division of Natural Sciences, International Christian University, Mitaka, Tokyo 181-8585, Japan.

ABSTRACT
Diabetic patients need particular care in case of infection, digestive disorder or external injury, because external stress often exasperates the glucose metabolism, which is known as "sick day management". In addition, severe trauma can be a cause of hyperglycemia with insulin resistance. In spite of critical component of the treatment, the precise mechanisms of how trauma develops posttraumatic diabetes remain unknown. Here, we ablated body wall muscles in Drosophila larvae by laser beam and found that the level of trehalose, the principal sugar circulating in the hemolymph or in the tissues of most insects, was increased. The model may provide a helpful tool to understand the relationship between trauma and sugar metabolism.

No MeSH data available.


Related in: MedlinePlus